Online citations, reference lists, and bibliographies.

A Simple, Rapid, And Sensitive DNA Assay Procedure.

Cesar Labarca, Kenneth Paigen
Published 1980 · Chemistry, Medicine
Cite This
Download PDF
Analyze on Scholarcy
Share
Abstract A simple and rapid assay for quantitative determinations of DNA in crude homogenates is described. The method is based on the enhancement of fluorescence seen when bisbenzimidazole (Hoechst 33258) binds to DNA. Crude homogenates in which chromatin has been dissociated with high salt buffer can be assayed directly and reliably in a few minutes. The dissociation of chromatin is critical to accurate determinations of DNA in biological materials using this method. The assay can detect as little as 10 ng of DNA with rather unsophisticated instrumentation.



This paper is referenced by
Growth Regulation by All-trans-retinoic Acid and 27
R-Y Shyu (2003)
10.1152/ajpregu.00085.2001
Dexamethasone decreases serum and liver IGF-I and maintains liver IGF-I mRNA in parenterally fed rats.
Karen R. Kritsch (2002)
10.1523/JNEUROSCI.12-01-00142.1992
Nuclear and cytoplasmic localization of basic fibroblast growth factor in astrocytes and CA2 hippocampal neurons
William R. Woodward (1992)
10.1007/s10529-006-9128-1
Microplate fluorescence assay for the quantification of double stranded DNA using SYBR Green I dye
Johanna Leggate (2006)
Reduction of islet pyruvate carbox ylase activity might be related to the development of type 2 diabetes mellitus in Agouti-K mice
Jaeseok Han (2010)
10.1210/endo.138.7.5222
Glutathione Peroxidase Degrades Intracellular Hydrogen Peroxide and Thereby Inhibits Intracellular Protein Iodination in Thyroid Epithelium.
Ragnar Ekholm (1997)
10.1095/biolreprod62.2.277
Expression of Carbohydrate Antigens in the Goat Uterus During Early Pregnancy and on Steroid-Treated Polarized Uterine Epithelial Cells In Vitro1
Jenny Powell (2000)
10.1159/000029908
Comparative Caustic and Biological Activity of Trichloroacetic and Glycolic Acids on Keratinocytes and Fibroblasts in vitro
Ljubiša Rakić (2000)
Arginine-vasopressin induces differentiation of skeletal myogenic cells and up-regulation of myogenin and Myf-5.
Clara Nervi (1995)
10.2144/98244rr03
Retroviral gene transfer in chondrogenic limb bud micromass cultures.
Ngaire Susan Stott (1998)
N-Methyl-D-Aspartate Increases Acetyicholine Release from Rat Striatum and Cortex : Its Effect is Augmented by Choline ’ MA ! L 1-1
(2006)
10.1002/0471143030.cba03ds07
Quantification of DNA and RNA with absorption and fluorescence spectroscopy.
Sean Robert Gallagher (2000)
10.1007/BF02209011
Isoproterenol-induced gastric mucosal protection from bile acid
David W. Mercer (1995)
10.1152/japplphysiol.00317.2003
Atrophy responses to muscle inactivity. I. Cellular markers of protein deficits.
Fadia Haddad (2003)
10.1007/s003359900259
Megencephaly: a new mouse mutation on chromosome 6 that causes hypertrophy of the brain
Leah Rae Donahue (2009)
10.1038/sj.cdd.4400477
Adenovirus-mediated expression of Fas ligand induces apoptosis of human prostate cancer cells
Tammy E. Hedlund (1999)
10.4081/836
Proliferation characteristics and polyploidization of cultured myofibroblasts from a patient with fibroblastic rheumatism.
Cristiano Lanni (2003)
10.1186/1477-7827-1-16
Synchronous onset of oestradiol-17β secretion by Meishan conceptuses
A. R. Pickard (2003)
10.1016/j.actbio.2008.09.004
Copper nanoparticle cues for biomimetic cellular assembly of crosslinked elastin fibers.
Chandrasekhar R Kothapalli (2009)
10.1007/BF00492587
Accurate determination of DNA content in single cell nuclei stained with Hoechst 33258 fluorochrome at high salt concentration
Tetsuya Araki (1987)
10.1007/BF02464897
Vitamin B12 transport inEscherichia coli K12 does not require thebtuE gene of thebtuCED operon
Clément R. Rioux (2006)
10.1007/BF00020128
The nucleosomal repeat length of pea (Pisum sativum) chromatin changes during germination
M. Angeles Ull (1986)
Consuming a Diet Enriched with Choline , UMP , and DHA Improves Memory in Rodents when these Compounds Increase Phospholipids
Sarah Holguin (2008)
10.1186/1471-2121-6-4
Extracellular degradation of lipoprotein lipase in rat adipose tissue
Gengshu Wu (2004)
10.2144/98243st03
Bacterial growth medium that significantly increases the yield of recombinant plasmid.
Harry M. Duttweiler (1998)
10.1007/BF00226207
Stable transformation of Sorghum bicolor protoplasts with chimeric neomycin phosphotransferase II and β-glucuronidase genes
M. Battraw (2004)
10.1016/j.cbi.2020.109059
Ghrelin regulates adipose tissue metabolism: Role in hepatic steatosis.
Karuna Rasineni (2020)
10.1152/ajpcell.00164.2005
Temporal changes of multiple redox couples from proliferation to growth arrest in IEC-6 intestinal epithelial cells.
Matias S. Attene-Ramos (2005)
10.1159/000107724
Intestinal Toxicity Induced by 5-Fluorouracil in Pigs: A New Preclinical Model
Manuel Manzano (2007)
10.1177/1934578X0600100807
Induction Effects of Apigenin, Luteolin and Vinpocetin on Neutral Endopeptidase (NEP) and Angiotensin-Converting Enzyme Activity (ACE) of SK-N-SH Cells
Shereen Ayoub (2006)
10.1007/BF02814391
Genetic and biochemical analysis of photolyase mutants ofChlamydomonas reinhardtii
D. B. Munce (2008)
10.1023/A:1007170622699
Glucocorticoids and TGF-β1 Synergize in Augmenting Fibroblast Mediated Contraction of Collagen Gels
Fu-Qiang Wen (2004)
See more
Semantic Scholar Logo Some data provided by SemanticScholar