Online citations, reference lists, and bibliographies.
← Back to Search

A Rapid Boiling Method For The Preparation Of Bacterial Plasmids.

D. Holmes, M. Quigley
Published 1981 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Abstract A simple and rapid method for preparing plasmids for restriction enzyme analysis has been developed. Bacteria are boiled for 15–40 s and an insoluble clot of genomic DNA and debris is removed by low-speed centrifugation. Plasmids are recovered from the supernatant by isopropanol precipitation and can be resuspended in buffer and immediately restricted. A 5-ml bacterial culture yields enough plasmids for many restriction enzyme digestions, but a single colony on a petri dish or a 0.5-ml miniculture will suffice for a few experiments. In addition, the procedure can be readily adapted for the preparation of plasmids from liter cultures with quantitative yields.
This paper references



This paper is referenced by
Zur Struktur und Funktion regulatorischer Elemente des cbb-Regulons in Ralstonia eutropha
T. Jeffke (2001)
10.1007/BF02100207
Variation in salmonid mitochondrial DNA: Evolutioinary constraints and mechanisms of substitution
W. Thomas (2005)
10.1111/j.1751-1097.1990.tb01793.x
SEQUENCE‐SPECIFICITY OF THE ALKALI‐SENSITIVE LESIONS INDUCED IN DNA BY HIGH‐INTENSITY ULTRAVIOLET LASER RADIATION
O. Kovalsky (1990)
10.1128/AEM.58.6.1952-1961.1992
Molecular analyses of the lactococcin A gene cluster from Lactococcus lactis subsp. lactis biovar diacetylactis WM4.
G. W. Stoddard (1992)
10.1016/0300-9084(90)90084-T
Efficient electrotransformation of Enterococcus hirae with a new Enterococcus-Escherichia coli shuttle vector.
M. Solioz (1990)
10.1128/JB.167.3.870-880.1986
Gene for an immunoglobulin-binding protein from a group G streptococcus.
S. Fahnestock (1986)
10.1007/978-3-642-74095-4_16
The Molecular Epidemiology of Antimicrobial Resistance
D. Shlaes (1989)
10.1007/BF00270973
Species-specific repetitive DNA used to identify interspecific somatic hybrids
M. W. Saul (2004)
10.1007/978-3-0348-9361-9_7
Nif mutants of azospirillum brasilense: evidence for a nif of a type regulation
F. Pedrosa (1983)
10.1016/B978-0-12-418687-3.00010-0
Isolation of plasmid DNA from bacteria.
L. Andreou (2013)
10.1186/1756-0500-3-150
Development and validation of a Xanthomonas axonopodis pv. citri DNA microarray platform (XACarray) generated from the shotgun libraries previously used in the sequencing of this bacterial genome
L. Moreira (2010)
Protein Import into Chloroplasts
Lea Vojta (2006)
10.1128/JB.178.19.5762-5767.1996
MobB protein stimulates nicking at the R1162 origin of transfer by increasing the proportion of complexed plasmid DNA.
T. Perwez (1996)
10.1007/BF01570757
Transfer of genes fromPseudomonas saccharophila to construct xylose-utilizing strains ofAlcaligenes eutrophus
B. Buchholz (2005)
10.1111/J.1432-1033.1994.00541.X
Molecular characterization of Escherichia coli malate synthase G. Differentiation with the malate synthase A isoenzyme.
I. Molina (1994)
10.1002/YEA.320090711
Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine‐specific permease
H. Sychrová (1993)
10.1007/BF00352435
Complementation of Saccharomyces cerevisiae acid phosphatase mutation by a genomic sequence from the yeast Yarrowia lipolytica identifies a new phosphatase
B. Tréton (2004)
10.1016/0147-619X(90)90020-D
Cloning and sequence of IS1000, a putative insertion sequence fromThermus thermophilus HB8
M. K. Ashby (1990)
10.1128/AEM.58.3.1050-1053.1992
Genetic Analysis of Rhizobium leguminosarum bv. Phaseoli Mutants Defective in Nodulation and Nodulation Suppression.
M. George (1992)
10.1007/BF00216384
Cloning of the β2-microglobulin gene in the zebrafish
H. Ono (2004)
10.3109/10425179209030967
DNA sequence characterization of the G gene region of bacteriophage Mu.
C. A. Baxa (1992)
10.1016/0378-1119(88)90392-7
Effects of dominant-negative lac repressor mutations on operator specificity and protein stability.
J. L. Betz (1988)
10.1007/BF00331315
Integrative suppression of dnaA46 by broad host-range plasmid R1162
M. Brasch (2004)
10.1016/0003-2697(87)90211-9
Determination of plasmid copy number by the "boiling" method.
I. Ivanov (1987)
10.1016/0092-8674(87)90761-6
The functions and relationships of Ty-VLP proteins in yeast reflect those of mammalian retroviral proteins
S. Adams (1987)
10.1016/0923-2508(91)90078-O
Nucleotide sequence of the genes coding for minor fimbrial subunits of the F1C fimbriae of Escherichia coli.
I. van Die (1991)
10.1007/BF00021329
Conservation of IS66 homologue of octopine Ti plasmid DNA in Rhizobium fredii plasmid DNA
N. Ramakrishnan (2004)
10.1007/BF00269855
Close linkage in Pseudomonas stutzeri of the structural genes for respiratory nitrite reductase and nitrous oxide reductase, and other essential genes for denitrification
A. Jüngst (2004)
10.1007/BF00224200
Assessment of the degree and the type of restriction fragment length polymorphism in barley (Hordeum vulgare)
A. Graner (2004)
10.1021/BI00475A014
Cloning and expression of the luxY gene from Vibrio fischeri strain Y-1 in Escherichia coli and complete amino acid sequence of the yellow fluorescent protein.
T. Baldwin (1990)
10.1016/B978-0-12-274162-3.50028-1
INSECTICIDAL ACTIVITY OF BACILLUS THURINGIENSIS CHIMERIC PROTOXINS
K. C. Raymond (1990)
10.1016/0167-4781(89)90146-2
Developmentally regulated late mRNAs in the encystment of Physarum polycephalum plasmodia.
L. Savard (1989)
See more
Semantic Scholar Logo Some data provided by SemanticScholar