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Anti-poly(L).poly(C) Antibody Bound To Cellulose And Its Use In The Specific Separation Of Double-stranded RNAs.

Y. Kitagawa, E. Okuhara
Published 1981 · Chemistry, Medicine

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Abstract Antibodies specific to double-stranded RNAs were purified from complexes of poly(A)·poly(U) and anti-poly(I)·poly(C) antibodies by a method of dissociation with 5% dimethylsulfoxide-alkali solution and DEAE-cellulose column chromatography. The purified antibodies were coupled to CNBr-activated cellulose. Synthetic and naturally occurring double-stranded RNAs were bound specifically by the antibody-linked cellulose and were eluted with alkali solution (pH 10.5) or 10% dimethylsulfoxide. The antibody-linked cellulose column appeared to be of practical value for the separation of rice dwarf virus ribonucleic acids from rice plant nucleic acids.
This paper references
10.1038/206779A0
Chromatography of Nucleic Acids on Hydroxyapatite
G. Bernardi (1965)
10.1016/0042-6822(72)90245-0
Double-stranded RNA in hamster, chick, and mosquito cells infected with Sindbis virus.
V. Stollar (1972)
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.
P. Cuatrecasas (1970)
10.1126/SCIENCE.622553
Double-stranded RNA is present in extracts of tobacco plants infected with tobacco mosaic virus.
K. Derrick (1978)
10.1016/s0021-9258(19)43202-x
Detection of inosine-containing transfer ribonucleic acid species by affinity chromatography on columns of anti-inosine antibodies.
H. Inouye (1973)
10.1016/0003-2697(74)90139-0
A simplified method for cyanogen bromide activation of agarose for affinity chromatography.
S. March (1974)
10.1016/0042-6822(72)90137-7
Immunochemical detection of double-stranded ribonucleic acid in leaves of sugar cane infected with Fiji disease virus.
R. I. Francki (1972)
10.1016/0042-6822(70)90270-9
Immunofluorescent demonstration of double-stranded RNA in the cytoplasm of Sindbis virus-infected cells.
B. D. Stollar (1970)
10.1093/OXFORDJOURNALS.JBCHEM.A133136
Demonstrations of the production of specific antibodies to poly(I).poly(C) in rabbits.
Y. Kitagawa (1980)
10.1021/BI00689A018
Detection and purification of isoaccepting tRNAPhe species containing Y base by affinity chromatography on columns of anti-Y antibodies
R. Salomon (1975)
10.1016/0005-2787(69)90275-5
Chromatography of nucleic acids on hydroxyapatite. 3. Chromatography of RNA and polyribonucleotides.
G. Bernardi (1969)
10.1016/0005-2787(74)90219-6
Rat liver double-stranded RNA from nuclei and whole cells: Isolation, purification and characterization
R.Parry Monckton (1974)
10.1620/TJEM.122.337
Immunogenicity of rice dwarf virus-ribonucleic acid.
Y. Kitagawa (1977)
10.1021/BI00584A025
Antigody-nucleic acid complexes. Inhibition of translation of silkmoth chorion messenger ribonucleic acid with antibodies specific for 7-methylguanosine.
T. Munns (1979)
10.1016/0042-6822(73)90421-2
Detection of mycoviruses using antiserum specific for dsRNA.
E. Moffitt (1973)
10.1126/SCIENCE.26972
Immunofluorescent detection of nuclear double-stranded RNA in situ in Vero and mosquito cells.
B. D. Stollar (1978)
10.1016/0022-2836(70)90140-3
Segments of genome of viruses containing double-stranded ribonucleic acid.
I. Fujii-Kawata (1970)
10.1016/0022-1759(76)90166-6
A method for producing anti-DNA antibodies in ascitic fluid of mice.
Y. Kitagawa (1976)



This paper is referenced by
10.1007/BF01411403
Absence of apoptosis in somatotropinomas treated with octreotide
Y. Saitoh (2005)
10.1007/BF01808885
Loss and apoptosis of smooth muscle cells in intracranial aneurysms studies with in situ DNA end labeling and antibody against single-stranded DNA
T. Sakaki (2005)
10.1016/0165-3806(94)90022-1
Apoptotic degeneration in the arhinencephalic brain of the mouse mutant Pdn/Pdn.
H. Keino (1994)
10.1007/BF00315834
Antibody against single-stranded DNA detects both programmed cell death and drug-induced apoptosis
I. Naruse (2004)
10.1111/j.1365-2249.1991.tb05683.x
Antigen DNA isolated from immune complexes in plasma of patients with systemic lupus erythematosus hybridizes with the Escherichia coli lac Z gene
K. Terada (1991)
10.1016/0022-1759(87)90360-7
Specific purification of monoclonal anti-DNA antibodies from culture medium using a DNA-coupled Sepharose 4B affinity column.
Y. Kitagawa (1987)
10.1016/0006-291X(91)90523-A
Demonstration of extrinsic DNA from immune complexes in plasma of a patient with systemic lupus erythematosus.
K. Terada (1991)
10.1002/JCP.1041240103
The fate of DNA originally existing in the zygote nucleus during achromosomal cleavage of fertilized echinoderm eggs in the presence of aphidicolin: Microscopic studies with anti‐DNA antibody
H. Yamada (1985)
10.1016/0022-1759(88)90093-2
Recovery of DNA fragments specifically bound by monoclonal anti-DNA antibody.
M. Fukada (1988)
10.1016/0022-1759(93)90269-D
Production of a monoclonal antibody reactive with poly(A) and its application in the isolation of poly(A)+ RNA.
Y. Kitagawa (1993)
10.1038/294417A0
Antibodies to left-handed Z-DNA bind to interband regions of Drosophila polytene chromosomes
A. Nordheim (1981)
10.1016/j.ab.2018.10.021
Development of a homogeneous time-resolved fluorescence assay for detection of viral double-stranded RNA
Motomichi Fujita (2019)
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