Online citations, reference lists, and bibliographies.
← Back to Search

The Influence Of Membrane Composition On The Solubilizing Effects Of Triton X-100.

M. Urbaneja, J. Nieva, F. Goñi, A. Alonso
Published 1987 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Multilamellar liposomes containing pure phosphatidylcholine (PC) or mixtures of PC with cholesterol, cholesteryl palmitate, beta-carotene, cardiolipin, phosphatidylethanolamine or gramicidin A have been treated with the detergent Triton X-100. Solubilization has been monitored as a decrease in turbidity of the liposome suspension, and also by determination of bilayer components in the solubilized fraction. The same solubilization pattern is found for unsaturated (egg yolk) or saturated (dimyristoyl) PC. Similar results are also found when dimyristoyl PC is solubilized above or below its gel-to-fluid transition temperature. Cholesterol solubilizes in parallel with PC; gramicidin A is solubilized preferentially to this phospholipid and the non-polar lipids cholesteryl palmitate or beta-carotene remain insoluble at detergent concentrations producing complete PC solubilization. Addition of cardiolipin or phosphatidylethanolamine does not seem to alter the general pattern of PC solubilization. Phosphatidylethanolamine is less soluble than PC, while cardiolipin solubilizes at the same detergent concentrations than PC. These results are considered in relation to previous studies with natural membranes.
This paper references
10.1016/0005-2736(74)90292-2
Purification and characterization of (Na+ plus K+ )-ATPase. 3. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate.
P. Jørgensen (1974)
10.1016/0304-4157(75)90016-7
Solubilization of membranes by detergents.
A. Helenius (1975)
10.1007/BF00745012
Triton X-100 solubilization of mitochondrial inner and outer membranes
J. Gurtubay (1980)
10.1016/0014-5793(85)80541-X
The effect of bilayer order and fluidity on detergent‐induced liposome fusion
R. Sáez (1985)
10.1016/0005-2736(86)90333-0
Interaction of nonionic detergents with phospholipids in hepatic microsomes at subsolubilizing concentrations as studied by 31P-NMR.
T. Bayerl (1986)
10.1021/AC60178A040
A Stable Reagent for the Liebermann-Burchard Reaction. Application to Rapid Serum Cholesterol Determination
T. Huang (1961)
10.1016/0005-2736(82)90510-7
Gramicidin induces the formation of non-bilayer structures in phosphatidylcholine dispersions in a fatty acid chain length dependent way
C.J.A. van Echteld (1982)
10.1016/0005-2760(69)90241-0
Partial delipidation of human serum β-lipoprotein by sodium decyl sulfate
A. Gotto (1969)
Studies on cytochrome oxidase. 8. Preparation and some properties of cardiac cytochrome oxidase.
M. Kuboyama (1972)
10.1016/0005-2736(87)90301-4
Kinetic studies on the interaction of phosphatidylcholine liposomes with Triton X-100.
A. Alonso (1987)
10.1016/0005-2736(83)90102-5
Membrane-surfactant interactions. The effect of Triton X-100 on sarcoplasmic reticulum vesicles.
A. Prado (1983)
10.1021/BI00789A019
Removal of lipids from human plasma low-density lipoprotein by detergents.
A. Helenius (1971)
10.1021/BI00510A006
Interactions between sarcoplasmic reticulum calcium adenosintriphosphatase and nonionic detergents.
W. L. Dean (1981)
10.2307/3226118
The molecular biology of cell membranes
P. Quinn (1976)
10.1016/0014-5793(81)80287-6
Lysis and reassembly of sonicated lecithin vesicles in the presence of triton X‐100
A. Alonso (1981)
10.1016/s0021-9258(18)70226-3
Phosphorus assay in column chromatography.
G. R. Bartlett (1959)
10.1021/BI00595A003
Effect of alterations in the amphipathic microenvironment on the conformational stability of bovine opsin. 1. Mechanism of solubilization of disk membranes by the nonionic detergent, octyl glucoside.
G. W. Stubbs (1978)
10.1016/s0021-9258(19)63820-2
Purification and properties of an adenosine triphosphatase from sarcoplasmic reticulum.
D. Maclennan (1970)
10.1016/0005-2736(74)90254-5
Differential solubilization of proteins, phospholipids, and cholesterol of erythrocyte membranes by detergents.
F. H. Kirkpatrick (1974)
10.1002/JSS.400010308
Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents.
J. Yu (1973)
10.1016/0009-3084(80)90002-X
Distearoylphosphatidyl choline/ammoniohexanoate surfactant interactions
Y.-C. Fu (1980)
10.1016/0165-022X(85)90026-0
Colorimetric assay of gramicidin A in the presence of surfactants and phospholipids.
M. Urbaneja (1985)
10.1016/0304-4157(83)90004-7
Solubilization of phospholipids by detergents. Structural and kinetic aspects.
D. Lichtenberg (1983)
10.1111/J.1432-1033.1986.TB10088.X
The interaction of phosphatidylcholine bilayers with Triton X-100.
F. Goñi (1986)
10.1021/BI00550A029
Action of detergents on membranes: differences between lipid extracted from red cell ghosts and from red cell lipid vesicles by Triton X-100.
R. MacDonald (1980)
10.1021/BI00673A025
The binding of deoxycholate, Triton X-100, sodium dodecyl sulfate, and phosphatidylcholine vesicles to cytochrome b5.
N. Robinson (1975)
10.1021/BI00791A025
Disruption of low- and high-density human plasma lipoproteins and phospholipid dispersions by 1-anilinonaphthalene-8-sulfonate.
R. Muesing (1971)
10.1016/s0021-9258(19)43628-4
Isolation and characterization of a lysolecithin-adenosine triphosphatase complex from lobster muscle microsomes.
D. Deamer (1973)
10.1016/s0021-9258(18)50811-5
Detergent properties of water-soluble choline phosphatides. Selective solubilization of acyl-CoA:lysolecithin acyltransferase from thymocyte plasma membranes.
H. U. Weltzien (1979)



This paper is referenced by
10.1016/S0005-2736(98)00095-9
Detergent solubilisation of phospholipid bilayers in the gel state: the role of polar and hydrophobic forces.
S. Patra (1998)
10.1134/S0022093017040068
The role of interspecies differences in the ratio of choline-containing phospholipid fractions of rodent erythrocytes in response of these cells to the effect of membranotropic compounds
O. Shevchenko (2017)
10.1023/A:1016043431778
Interaction of Polyethyleneglycol-Phospholipid Conjugates with Cholesterol-Phosphatidylcholine Mixtures: Sterically Stabilized Liposome Formulations
R. K. Bedu-Addo (2004)
10.1016/0014-5793(92)80424-F
Effective detergent/lipid ratios in the solubilization of phosphatidylcholine vesicles by Triton X‐100
M. A. Partearroyo (1992)
10.1093/JXB/50.341.1715
Proteoliposomes and plant transport proteins
G. Hanke (1999)
10.1016/j.chemphyslip.2015.08.011
Lipids that determine detergent resistance of MDCK cell membrane fractions.
Marco M. Manni (2015)
10.1016/S0378-5173(98)00345-7
Surfactants in membrane solubilisation.
M. Jones (1999)
10.1021/BI00471A012
States of aggregation and phase transformations in mixtures of phosphatidylcholine and octyl glucoside.
S. Almog (1990)
10.1016/0021-9797(92)90425-L
Systematic study on the solubilization of phospholipid vesicles by various surfactants
T. Inoue (1992)
10.1016/j.febslet.2009.07.046
Cholesterol reverts Triton X‐100 preferential solubilization of sphingomyelin over phosphatidylcholine: A 31P‐NMR study
Hasna Ahyayauch (2009)
10.1016/j.bbalip.2008.09.002
Phase diagrams of lipid mixtures relevant to the study of membrane rafts.
F. Goñi (2008)
10.1111/J.1432-1033.1990.TB15449.X
The interaction of Triton X-100 with purple membranes. Detergent binding, spectral changes and membrane solubilization.
J. M. González-Mañas (1990)
10.1016/S0005-2736(97)00233-2
Solubilization of planar bilayers with detergent.
G. Csucs (1998)
10.1023/A:1015924124180
Nebulization of Liposomes. I. Effects of Lipid Composition
R. Niven (2004)
10.1016/0006-2952(90)90399-6
Surfactant-induced cell toxicity and cell lysis. A study using B16 melanoma cells.
M. Aránzazu Partearroyo (1990)
10.1016/0005-2736(94)90197-X
Solubilization by Triton X-100 makes possible complete recovery of lipids from liposomes in enzymatic assay.
A. Nagayasu (1994)
10.1074/jbc.273.2.1150
Cholesterol and Sphingolipid Enhance the Triton X-100 Insolubility of Glycosylphosphatidylinositol-anchored Proteins by Promoting the Formation of Detergent-insoluble Ordered Membrane Domains*
R. Schroeder (1998)
10.1016/0005-2736(88)90234-9
Surfactant-induced release of liposomal contents. A survey of methods and results.
J. Ruíz (1988)
10.1529/BIOPHYSJ.105.067710
Detergent-resistant, ceramide-enriched domains in sphingomyelin/ceramide bilayers.
J. Sot (2006)
10.1016/S0005-2736(97)00265-4
The aminosterol antibiotic squalamine permeabilizes large unilamellar phospholipid vesicles.
B. Selinsky (1998)
10.1016/S0304-4157(00)00007-1
Insolubility of lipids in triton X-100: physical origin and relationship to sphingolipid/cholesterol membrane domains (rafts).
E. London (2000)
10.1016/S0005-2736(00)00309-6
Mixed micelles and other structures in the solubilization of bilayer lipid membranes by surfactants.
M. Almgren (2000)
Semantic Scholar Logo Some data provided by SemanticScholar