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On The Mechanism Of Pancreatic Lipolysis Of Glycerides
Published 1954 · Chemistry
Abstract During hydrolysis of triglycerides of long chain fatty acids by pancreatic juice from rat an exchange occurs between glyceride fatty acids and liberated free fatty acids. In thi] exchange only fatty acids in the 1- and 3-position of the glyceride take part and these fatty acids rapidly come into equilibrium with the free fatty acids. The exchange between the glyceride fatty acids and the free fatty acids is at least partly due to a resynthesis of glyceride ester bonds occuring simultaneously with the hydrolysis. This resynthesis has been evidenced for the step 1.2-diglyceride to triglyceride. Under the conditions of the experiments, resembling those prevailing during digestion in the lumen of the small intestine, no resynthesis takes place from glycerol and fatty acids by the action of pancreatic juice and the overall hydrolysis of glycerides accordingly proceeds to completion in vitro . The results of this investigation indicate that the hydrolysis of glycerides by pancreatic juice enzyme proceeds via the 1.2-diglyceride to both 1- and 2- monoglycerides, the hydrolysis to the 2-monoglyceride prevailing. The pH/activity curves for the hydrolysis and resynthesis of glyceride ester bonds by pancreatic juice enzyme with olive oil as substrate in the absence of bile acids are parallel and show a maximum at pH about 8. Addition of Ca ions accelerates the rate of hydrolysis of triglycerides by pancreatic juice both in acid and alkaline media. The increased rate of hydrolysis brought about by Ca ions is parallel to a decreased rate of resynthesis of glyceride ester bonds. Different fatty acids are built into glycerides during hydrolysis with pancreatic enzyme at different rates.