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The Axonal Transport Of Slowly Migrating (3H)leucine Labelled Proteins And The Regeneration Rate In Regenerating Hypoglossal And Vagus Nerves Of The Rabbit.
M. Frizell, J. Sjöstrand
Published 1974 · Chemistry, Medicine
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Abstract The axonal transport of slowly migrating [3H]leucine labelled proteins was studied in regenerating hypoglossal and vagus nerves of the rabbit 1–4 weeks after a nerve crush. In normal nerves, as well as in the nerves contralateral to nerve crush, a transport rate of 4–5 mm/day for the hypoglossal nerve and 20–25 mm/day for the vagus nerve was calculated. The axonal transport of slowly migrating labelled proteins was increased in the regenerating hypoglossal nerve as compared to the contralateral 1 week after a nerve crush; this was mainly due to an increased transport rate. In contrast, the axonal transport of slowly migrating labelled proteins was decreased in the regenerating vagus nerve as compared to the contralateral nerve 1 week after a nerve crush. The results indicate a decreased transport rate and a decreased amount of material transported on the regenerating side. Changes in axonal transport in both hypoglossal and vagus nerves were less pronounced after 4 weeks of regeneration. Rapidly migrating [3H]leucine labelled proteins were transported into regenerating hypoglossal and vagus nerves distal to the crush zone, indicating axonal transport in the growing axons. The regeneration rate was estimated to be about 3 mm/day in the vagus nerve after 2–4 weeks of regeneration and about 4 mm/day in the hypoglossal nerve after 1 week of regeneration. The levels of acetyl-CoA: choline O-acetyltransferase (ChAc, E.C. 220.127.116.11) and acetylcholinesterase (AChE, E.C. 18.104.22.168) activity were decreased in regenerating vagus nerve the first week after a nerve crush.
This paper references
ULTRASTRUCTURE AND FUNCTION OF GROWTH CONES AND AXONS OF CULTURED NERVE CELLS
K. Yamada (1971)
Rate of regeneration of peripheral nerves in man
H. Seddon (1943)
TRANSPORT, TURNOVER AND DISTRIBUTION OF CHOLINE ACETYLTRANSFERASE AND ACETYLCHOLIN‐ESTERASE IN THE VAGUS AND HYPOGLOSSAL NERVES OF THE RABBIT
F. Fonnum (1973)
Effects of vagotomy on the cholinesterase content of the preganglionic innervation of the rat heart.
V. Navaratnam (1968)
Cholinesterases in degenerating and regenerating peripheral nerves.
C. Sawyer (1946)
A light and electron microscopic study of the cellular response to axonal injury in the superior cervical ganglion of the rat
M. R. Matthews (1972)
DER CHOLINESTERASEGEHALT MOTORISCHER NERVENZELLEN WÄHREND DER AXONALEN REAKTION
H. G. Schwarzacher (1958)
Distribution-Free Statistical Tests
J. V. Bradley (1968)
Probability tables for individual comparisons by ranking methods.
F. Wilcoxon (1947)
The axonal transport of (3H)fucose labelled glycoproteins in normal and regenerating peripheral nerves.
M. Frizell (1974)
Changes in the rate of axoplasmic transport during postnatal development of the rabbit's optic nerve and tract.
A. Hendrickson (1971)
Transport of s-100 protein in mammalian nerve fibers and transneuronal signals.
N. Miani (1971)
THE CHEMICAL CHANGES IN REGENERATING NEURONS
S.‐O. Brattgårrd (1957)
FAST AND SLOW PHASES OF AXOPLASMIC FLOW IN VENTRAL ROOT NERVE FIBRES 1
S. Ochs (1969)
Accumulation and loss of noradrenaline central to a constriction on adrenergic nerves.
F. C. Boyle (1970)
AXOPLASMIC STREAMING IN REGENERATING AND IN NORMAL NERVE FIBRES.
L. Lubińska (1964)
Regeneration of non-medullated nerve fibres.
D. H. Evans (1954)
Transport of protein in goldfish optic nerve during regeneration.
B. Grafstein (1969)
FINE STRUCTURE OF NERVE FIBERS AND GROWTH CONES OF ISOLATED SYMPATHETIC NEURONS IN CULTURE
M. Bunge (1973)
The rate of regeneration of the vagus nerve of the cat.
L. Guth (1966)
SYNTHESIS, MIGRATION AND TURNOVER OF PROTEIN IN RETINAL GANGLION CELLS
J. O. Karlsson (1971)
THE RESPONSE OF VENTRAL HORN NEURONS TO AXONAL TRANSECTION
D. Price (1972)
Experiments on the mechanism of nerve growth.
P. Weiss (1948)
Radiochemical micro assays for the determination of choline acetyltransferase and acetylcholinesterase activities.
F. Fonnum (1969)
Effect of cut, ligation or crush on the synthesis and transport of amine storage granules in rat sciatic nerve.
L. Karlström (1970)
Choline acetylase in antero‐ and retro‐grade degeneration of a cholinergic nerve
C. Hebb (1956)
Proximo-Distal Movement along the Axon of Protein synthesized in the Perikaryon of Regenerating Neurons
N. Miani (1960)
ANALYSIS OF THE SOMATO‐AXONAL MOVEMENT OF PHOSPHOLIPIDS IN THE VAGUS AND HYPOGLOSSAL NERVES
N. Miani (1963)
Model for Membrane Movements in the Neural Growth Cone
D. Bray (1973)
Axonal transport in growing and mature retinal ganglion cells.
J. Sjöstrand (1973)
Effects of axonal injury on norepinephrine, tyrosine hydroxylase and monoamine oxidase levels in sympathetic ganglia.
T. B. Cheah (1973)
Changes in axonal transport during regeneration of feline ventral roots.
C. Carlsson (1971)
Respiratory enzyme activities in neurons and glial cells of the hypoglossal nucleus during nerve regeneration.
A. Hamberger (1966)
Observations on the nucleolar and total cell body nucleic acid of injured nerve cells
W. E. Watson (1968)
THE RATE OF REGENERATION OF NERVE
E. Gutmann (1942)
TRANSPORT OF PROTEINS, GLYCOPROTEINS AND CHOLINERGIC ENZYMES IN REGENERATING HYPOGLOSSAL NEURONS 1
M. Frizell (1974)
Fast and slow components of axoplasmic transport in the hypoglossal and vagus nerves of the rabbit.
J. Sjöstrand (1970)
Chromatolysis in the hypoglossal nucleus of the rat: An electron microscopic analysis
J. Kirkpatrick (1968)
Role of slow axonal transport in nerve regeneration.
B. Grafstein (1971)
Changes in axonal flow during regeneration of mammalian motor nerves.
G. Kreutzberg (1971)
DISTRIBUTION OF LEUCINE-3H DURING AXOPLASMIC TRANSPORT WITHIN REGENERATING NEURONS AS DETERMINED BY ELECTRON-MICROSCOPE RADIOAUTOGRAPHY
T. Lentz (1972)
Changes in acetylcholinesterase, acid phosphatase and beta glucuronidase proximal to a nerve crush.
J. Johnson (1970)
This paper is referenced by
Nerve repair and axonal transport: Outgrowth delay and regeneration rate after transection and repair of rabbit hypoglossal nerve
N. Danielsen (1986)
Elimination of microglia in mouse spinal cord alters the retrograde CNS plasticity observed following peripheral axon injury
Jessika M. Hutchinson (2019)
The effect of a conditioning lesion on the regeneration of motor axons
I. Mcquarrie (1978)
Regeneration of motor axons in the rat sciatic nerve studied by labeling with axonally transported radioactive proteins
D. Forman (1978)
Early structural changes in the axoplasmic cytoskeleton after axotomy studied by cryofixation
K. Meller (2004)
Axonal transport of actin and regeneration rate in non-myelinated sensory nerve fibres
W. Mclean (1985)
Axoplasmic flow in myelinated and unmyelinated nerves
M. Somerville (1977)
Anterograde Components of Axonal Transport in Motor and Sensory Nerves in Experimental 2,5‐Hexanedione Neuropathy
H. Brændgaard (1986)
Fast axonal transport of labeled protein in sensory axons during regeneration
M. Bisby (1978)
Slow flow in regenerating C-fibers.
P. Cancalon (1983)
Slow axonal transport of proteins: Blockade by interruption of contact between cell body and axon
M. Frizell (1975)
RETROGRADE AXONAL TRANSPORT OF RAPIDLY MIGRATING LABELLED PROTEINS AND GLYCOPROTEINS IN REGENERATING PERIPHERAL NERVES
M. Frizell (1976)
FINE STRUCTURAL CHANGES IN NERVE CELL BODIES OF THE ADULT RABBIT DORSAL MOTOR VAGAL NUCLEUS DURING AXON REACTION
H. Aldskogius (1978)
Incorporation of axonally transported glycoproteins into axolemma during nerve regeneration
J. Griffin (1981)
Axonal growth during regeneration: a quantitative autoradiographic study
A. Tessler (1980)
The effect of ligation combined with section on anterograde axonal transport in rabbit hypoglossal nerve
M. Frizell (1982)
Alkaline ribonuclease activity is increased in rat sympathetic ganglia after nerve injury
D. J. Bates (2004)
Changes in the mitochondria in the initial part of the axon during regeneration
R. Dimova (2004)
Cryo-electron microscopy of nervous tissue. A review.
K. Meller (1992)
The role of adrenergic mechanisms in thermoregulatory control of blood flow through capillaries and arteriovenous anastomoses in the sheep hind limb
J. Haleš (2004)
General Properties of Axoplasmic Transport
D. Weiss (1982)
Fast axonal transport in motor nerve regeneration.
J. Griffin (1976)
Rapid axonal transport of labeled proteins in regenerating sensory and motor fibers of rabbit vagus nerve
W. Mclean (1976)
Influence of temperature on various mechanisms associated with neuronal growth and nerve regeneration
P. Cancalon (1985)
RNA METABOLISM IN THE GOLDFISH RETINA DURING OPTIC NERVE REGENERATION 1, 2
H. Burrell (1978)
Changes in Rapid Transport of Phospholipids in the Rat Sciatic Nerve During Axonal Regeneration
M. Alberghina (1983)
The formation of axonal sprouts in organ culture and their relationship to sprouting in vivo.
I. Duce (1980)
Descending optic nerve degeneration in primates.
H. Quigley (1977)
Retrograde Axonal Transport
M. Bisby (1980)
Impaired axonal regeneration in acrylamide intoxication.
J. Griffin (1977)
Radioautographic study of the axonal transport of proteins into the sensory nerve endings of avian mechanoreceptors
C. Chouchkov (1983)See more