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Structural Analysis Of Molecular Species Of Nocardomycolic Acids FromNocardia Erythropolis By The Combined System Of Gas Chromatography And Mass Spectrometry
Published 1972 · Biology, Medicine
Mycolic acids are high molecular fl-hydroxy fatty acids with long-chain branches in the o-position, which occur abundantly in certain groups of bacteria such as Mycobacteria [ 1, 2,3], Corynebacteria [4,5] and Nocardia [6,7,8] . The common structures of these acids have been well investigated. However, owing to the instability of mycolic acids at high temperature and the complexity of their homologues, gas chromatographic analysis of the individual acids has not yet been established. Recently, we have succeeded in separating the individual molecular species of the mycolic acids from Nocardia by the combination of gas chromatography and mass spectrometry as their trimethylsilyl derivatives. The present paper describes that the homologues of nocardomycolic acids from Nocardia erythropolis grown on a high glucose medium [9, lo] can be separated into 15 major acids according to their carbon numbers, which comprise saturated acids ran.l:;Ig from C,, to Ca6 with a smaller amounts of mon c 2s. In al! cases, the branches at the o-position are C, to C,,.