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Strong Ionic Interactions Between Mucins And Two Basic Proteins, Mucus Proteinase Inhibitor And Lysozyme, In Human Bronchial Secretions.

I. Van-Seuningen, N. Houdret, A. Hayem, M. Davril
Published 1992 · Chemistry, Medicine

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1. Mucins were isolated from sputum from a patient with chronic bronchitis and subjected to two different preparation procedures. 2. In the first procedure, CsBr density-gradient centrifugation gave rise to two well-separated fractions. Mucins recovered in the high-density fraction still contained mucus proteinase inhibitor (MPI) and lysozyme (LSZ). 3. Mucins were purified after a second step of CsBr density-gradient centrifugation or after gel-filtration chromatography with a buffer of high ionic strength, containing 0.5 M NaCl. 4. In the second procedure, trichloroacetic acid treatment of whole sputum followed by cation-exchange chromatography allowed the obtention of a non-retained fraction composed of mucins. 5. Gel-filtration in buffer containing 0.5 M NaCl, allowed the release of MPI and LSZ from mucins, thus confirming that interactions still occurred between those components. 6. The chemical compositions of the mucins isolated by the two above procedures were quite similar. 7. These data support the hypothesis of the existence of ionic interactions between basic amino acid residues of MPI and LSZ and acid residues of the carbohydrate chains of mucins in the secretions of the large airways. 8. These interactions could play a role in the protection of mucins against proteolysis and consequently in the maintenance of rheological properties of the mucus gel in disease.
This paper references
Lysozyme and lactoferrin in sputum from patients with chronic obstructive lung disease.
O. Harbitz (1984)
10.1016/0020-711X(82)90072-6
Binding of basic proteins to glycoproteins in human bronchial secretions.
C. E. Snyder (1982)
10.1042/BJ1670557
The separation and characterization of bronchial glycoproteins by density-gradient methods.
J. M. Creeth (1977)
The origin of DNA associated with mucus glycoproteins in cystic fibrosis sputum.
M. I. Lethem (1990)
Heterogeneity of the carbohydrate chains of sulfated bronchial glycoproteins isolated from a patient suffering from cystic fibrosis.
P. Roussel (1975)
10.1136/thx.36.2.108
Ultrastructural localisation of lactoferrin and glycoprotein in human bronchial glands.
D. Bowes (1981)
10.1016/0304-4165(86)90119-4
The high lipid content of respiratory mucins in cystic fibrosis is related to infection.
N. Houdret (1986)
10.1021/BI00533A017
Isolation, purification, and properties of respiratory mucus glycoproteins.
H. Woodward (1982)
10.1016/0003-2697(69)90383-2
Glycoprotein staining following electrophoresis on acrylamide gels.
R. M. Zacharius (1969)
10.1515/BCHM2.1977.358.1.583
Isolation and partial characterization of a low molecular weight acid stable protease inhibitor from human bronchial secretion.
K. Ohlsson (1977)
Antileukoprotease, its role in the human lung.
J. Kramps (1988)
10.1016/0009-8981(78)90342-X
Etude biochimique comparative des glycosaminoglycannes peptides du cartilage articulaire de la tete femorale humaine normale et arthrosique
M. Suzuki (1978)
10.1016/S0076-6879(72)25021-2
[19] Gas-liquid chromatographic analysis of constituent carbohydrates in glycoproteins.
Vernon N. Reinhold (1972)
10.1038/227680A0
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
10.1042/BJ1810717
An interaction between lysozyme and mucus glycoproteins. Implications for density-gradient separations.
J. M. Creeth (1979)
10.3109/01902148909062857
Physical properties of purified human respiratory mucus glycoproteins: effects of sodium chloride concentration on the aggregation properties and shape.
K. Chace (1989)
10.1172/JCI114171
Increased sulfation of glycoconjugates by cultured nasal epithelial cells from patients with cystic fibrosis.
P. W. Cheng (1989)
10.1016/0300-9084(88)90284-2
The complexity of mucins.
P. Roussel (1988)
10.1177/37.4.2926127
Tissue distribution of antileukoprotease and lysozyme in humans.
C. Franken (1989)
10.1111/J.1432-1033.1984.TB08273.X
Complex structure of human bronchial mucus glycoprotein.
H. Slayter (1984)
10.1093/NAR/14.20.7883
Isolation and sequence of a human gene encoding a potent inhibitor of leukocyte proteases.
G. Stetler (1986)
10.1515/BCHM3.1989.370.2.749
Evidence for the tight binding of human mucus proteinase inhibitor to highly glycosylated macromolecules in sputum.
I. Van-Seuningen (1989)
10.1128/IAI.56.3.703-704.1988
Kinetics of proteolysis of hog gastric mucin by human neutrophil elastase and by Pseudomonas aeruginosa elastase.
L. Poncz (1988)
10.1042/BJ0810384
Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.
D. Aminoff (1961)
10.1042/BJ0750435
The ultramicro determination of inorganic sulphte.
B. Spencer (1960)



This paper is referenced by
The isolation, purification, tissue localization and identification of a glycoprotein found in the crude mucus gel of patients with carcinoma of the stomach
Chirwa Nthato (2008)
10.1165/AJRCMB.13.2.7626283
Effect of dexamethasone on antigen-induced high molecular weight glycoconjugate secretion in allergic guinea pigs.
C. Savoie (1995)
10.3109/13813459209000712
Structure et fonctions sécrétoires de l'épithélium respiratoire
J. Jacquot (1992)
10.1177/00220345000790020601
Molecular Mapping of Statherin- and Histatin-binding Domains in Human Salivary Mucin MG1 (MUC5B) by the Yeast Two-hybrid System
I. lontcheva (2000)
10.1111/J.1432-1033.1993.TB17575.X
Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis.
A. Klein (1993)
10.1164/AJRCCM.164.6.2012058
Differential properties of mucous glycoproteins in rat nasal epithelium. A comparison between allergic inflammation and lipopolysaccharide stimulation.
T. Shimizu (2001)
10.1177/10454411950060020501
Biochemical composition of human saliva in relation to other mucosal fluids.
L. Schenkels (1995)
10.1042/BJ3510421
Macromolecular organization of saliva: identification of 'insoluble' MUC5B assemblies and non-mucin proteins in the gel phase.
C. Wickström (2000)
Exhaled Breath Condensate in Obstructive Lung Diseases : A Methodological study
A. Davidsson (2009)
10.1074/jbc.273.22.13563
Interaction of Secretory Leukocyte Protease Inhibitor with Heparin Inhibits Proteases Involved in Asthma*
M. Fath (1998)
10.1146/ANNUREV.PHYSIOL.70.113006.100702
Structure and function of the polymeric mucins in airways mucus.
D. Thornton (2008)
10.1177/00220345970760020301
Concise Review: The Anti-HIV-1 Activity Associated with Saliva
N. Shine (1997)
10.1007/978-3-642-37250-6_1
Mucus, Goblet Cell, Submucosal Gland
Takeshi Shimizu (2013)
10.2500/ajr.2008.22.3185
Glycoconjugate Expression of Sinus Mucosa in Chronic Rhinosinusitis: A Lectin Histochemical Study
G. Berger (2008)
Otitis media with effusion : key factors.
A. Tasker (2003)
10.1034/J.1600-0722.2002.11175.X
Inhibition of HIV-1 IIIB and clinical isolates by human parotid, submandibular, sublingual and palatine saliva.
J. Bolscher (2002)
10.1023/A:1020867221861
Human airway mucin glycosylation: A combinatory of carbohydrate determinants which vary in cystic fibrosis
G. Lamblin (2004)
10.1111/j.1399-3038.1993.tb00087.x
Biochemical properties of aeroallergens: contributory factors in allergic sensitization?
G. Stewart (1993)
10.1016/S0928-0987(99)00032-9
Chitosans as absorption enhancers of poorly absorbable drugs. 3: Influence of mucus on absorption enhancement.
N. G. Schipper (1999)
10.1080/02713689808951215
Identification, origins and the diurnal role of the principal serine protease inhibitors in human tear fluid.
S. Sathe (1998)
10.1016/j.actbio.2018.08.023
Metal-organic frameworks, NH2-MIL-88(Fe), as carriers for ophthalmic delivery of brimonidine.
Se-na Kim (2018)
Mucus Hypersecretion, MUC genes and Mucins in Inflammatory Nasosinusal Diseases. Regulation by Proinflammatory and Antiinflammatory Agents
M. Antón (2008)
10.1177/194589240501900117
Glycoconjugate Expression in Normal Human Inferior Turbinate Mucosa: A Lectin Histochemical Study
G. Berger (2005)
A Novel Electrophoretic Variant of Human Tear Lysozyme with Altered Kinetic and Thermodynamic Properties
I. Ibrahimi (2010)
10.1128/AEM.01374-10
Integrity of Proteins in Human Saliva after Sterilization by Gamma Irradiation
S. Ruhl (2010)
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