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Partial Resistance Of Nascent Polypeptide Chains To Proteolytic Digestion Due To Ribosomal Shielding.

L. Malkin, A. Rich
Published 1967 · Biology, Medicine

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Abstract Reticulocyte polyribosomes with labeled nascent chains were subjected to low-temperature proteolytic digestion by four different enzymes. Approximately 50% of the radioactive material in the nascent chain was digested by each of these enzymes, but the hydrodynamic integrity of the ribosome was maintained. Disruption of the ribosome, however, led to the complete proteolysis of the nascent chain. Pulse experiments showed that the most recently synthesized part of the chain was resistant to proteolysis, presumably because it is shielded by the ribosome. The resistant fractions were characterized by Sephadex column fractionation and were found to contain approximately 30 to 35 amino acid residues. These experiments suggest that the site for adding amino acids may be in the interior of the ribosome.
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