Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Arachidonoyl Ethanolamide-[1,2-14C] As A Substrate For Anandamide Amidase.
R. Omeir, S. Chin, Y. Hong, D. G. Ahern, D. Deutsch
Published 1995 · Chemistry, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Arachidonoyl ethanolamide-[1,2-14C] was prepared and evaluated as a substrate for anandamide amidase in a radioenzymatic assay that does not require a thin layer chromatography separation step. Using this substrate the release of ethanolamine-[1,2-14C] is linear for approximately thirty minutes. Anandamide amidase exhibits maximal activity between pH 8 and pH 9 with a steep decline in activity at pH values below 6 and above 10. Arachidonoyl ethanolamide-[1,2-14C] was used for the assay of anandamide amidase from 10 micrograms to 100 micrograms protein, from cow brain homogenate, in a 0.2 ml incubation mixture. When plotted as a rectangular hyperbola of the steady-state Michaelis-Menten equation, an approximate Km of 30 +/- 7 microM and a Vmax of 198 +/- 13 nmoles ethanolamine formed per hour per mg protein homogenate was obtained.
This paper references
Isolation and structure of a brain constituent that binds to the cannabinoid receptor.
W. Devane (1992)
The cannabinoid receptor: biochemical, anatomical and behavioral characterization
A. Howlett (1990)
Effects of anandamide on cannabinoid receptors in rat brain membranes.
S. Childers (1994)
Two new unsaturated fatty acid ethanolamides in brain that bind to the cannabinoid receptor.
L. Hanuš (1993)
Inhibitors of arachidonoyl ethanolamide hydrolysis.
B. Koutek (1994)
Formation and inactivation of endogenous cannabinoid anandamide in central neurons
V. Marzo (1994)
New dawn of cannabinoid pharmacology.
W. Devane (1994)
Anandamide, an endogenous cannabinoid, inhibits calcium currents as a partial agonist in N18 neuroblastoma cells.
K. Mackie (1993)
Enzymatic synthesis and degradation of anandamide, a cannabinoid receptor agonist.
D. Deutsch (1993)
Catabolism of N‐Acylethanolamine Phospholipids by Dog Brain Preparations
V. Natarajan (1984)
Structure of a cannabinoid receptor and functional expression of the cloned cDNA
L. Matsuda (1990)
Properties of rat liver N-acylethanolamine amidohydrolase.
P. Schmid (1985)
Microsomal synthesis of fatty acid amides.
N. Bachur (1966)
(R)-methanandamide: a chiral novel anandamide possessing higher potency and metabolic stability.
V. Abadji (1994)
This paper is referenced by
Inhaled anandamide reduces leukotriene D4-induced airway obstruction in guinea pigs.
P. Stengel (2007)
N-Morpholino- and N-diethyl-analogues of palmitoylethanolamide increase the sensitivity of transfected human vanilloid receptors to activation by anandamide without affecting fatty acid amidohydrolase activity.
S. Vandevoorde (2003)
The endocannabinoid system as a novel target in the pathophysiology and treatment of depressive illness
M. Hill (2008)
Occurrence, Biosynthesis, and Metabolism of Endocannabinoids
K. Waku (2005)
Inhibition of fatty acid amide hydrolase and monoacylglycerol lipase by the anandamide uptake inhibitor VDM11: evidence that VDM11 acts as an FAAH substrate
S. Vandevoorde (2005)
Suppression of Amygdalar Endocannabinoid Signaling by Stress Contributes to Activation of the Hypothalamic-Pituitary-Adrenal Axis
M. Hill (2009)
Changes in brain levels of N‐acylethanolamines and 2‐arachidonoylglycerol in focal cerebral ischemia in mice
M. Degn (2007)
A high-throughput-compatible assay for determining the activity of fatty acid amide hydrolase.
S. Wilson (2003)
Inhibitors of anandamide breakdown.
D. Deutsch (1997)
Cannabinoid Properties of Methylfluorophosphonate Analogs 1
B. Martin (2000)
‘Entourage’ effects of N‐acyl ethanolamines at human vanilloid receptors. Comparison of effects upon anandamide‐induced vanilloid receptor activation and upon anandamide metabolism
D. Smart (2002)
Fatty acid amide hydrolase: biochemistry, pharmacology, and therapeutic possibilities for an enzyme hydrolyzing anandamide, 2-arachidonoylglycerol, palmitoylethanolamide, and oleamide.
C. Fowler (2001)
Trifluoromethyl ketone inhibitors of fatty acid amide hydrolase: a probe of structural and conformational features contributing to inhibition.
D. Boger (1999)
[The role of the endocannabinoid system in the regulation of endocrine function and in the control of energy balance in humans].
J. Komorowski (2007)
Novel inhibitors of fatty acid amide hydrolase.
S. Sit (2007)
Microglia produce and hydrolyze palmitoylethanolamide
G. Muccioli (2008)
Pharmacological properties of rat brain fatty acid amidohydrolase in different subcellular fractions using palmitoylethanolamide as substrate.
G. Tiger (2000)
Substituted 2-thioxoimidazolidin-4-ones and imidazolidine-2,4-diones as fatty acid amide hydrolase inhibitors templates.
G. Muccioli (2006)
High-Throughput Screening for the Discovery of Inhibitors of Fatty Acid Amide Hydrolase Using a Microsome-Based Fluorescent Assay
Y. Wang (2006)
A Bio-Psycho-Socio-Molecular Approach to Pain and Stress Management
T. Esch (2007)
The cloned rat hydrolytic enzyme responsible for the breakdown of anandamide also catalyzes its formation via the condensation of arachidonic acid and ethanolamine
G. Arreaza (1997)
Deletion of a proline‐rich region and a transmembrane domain in fatty acid amide hydrolase 1
G. Arreaza (1999)
The Cellular Uptake of Anandamide Is Coupled to Its Breakdown by Fatty-acid Amide Hydrolase*
D. Deutsch (2001)
Acidic Nonsteroidal Anti-inflammatory Drugs Inhibit Rat Brain Fatty Acid Amide Hydrolase in a pH-dependent Manner
C. Fowler (2003)
Chronic, noninvasive glucocorticoid administration suppresses limbic endocannabinoid signaling in mice
N. P. Bowles (2012)
A Double Whammy: Targeting Both Fatty Acid Amide Hydrolase (FAAH) and Cyclooxygenase (COX) To Treat Pain and Inflammation
R. Scarpelli (2016)
Identification of two serine residues involved in catalysis by fatty acid amide hydrolase.
R. Omeir (1999)
The fatty acid amide hydrolase (FAAH).
N. Ueda (2000)
Differences in the pharmacological properties of rat and chicken brain fatty acid amidohydrolase
C. Fowler (2000)
Endocannabinoids and N-acylethanolamines in translational pain research : from monoacylglycerol lipase to muscle pain
Nazdar Ghafouri (2013)
Cannabinoid properties of methylfluorophosphonate analogs.
B. Martin (2000)
Temporal changes in N‐acylethanolamine content and metabolism throughout the peri‐adolescent period
T. T. Lee (2013)See more