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Characterization Of Bacteriophage D6.
Published 1981 · Biology, Medicine
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Abstract Bacteriophage D6, described by Mise and Suzuki [J. Virol.6, 253–255 (1970)], is a temperate coliphage capable of generalized transduction. D6 resembles phage P1 morphologically and is slightly sensitive to anti-P1 serum. We have found that like P1, the D6 prophage is a plasmid, approximately 90 kb in size. The D6 prophage is compatible with both P1 and the closely related heteroimmune prophage P7, both of which are in incompatibility group Y. D6 DNA is restricted by the P1 and P7 prophages, but in the absence of restriction, D6 is able to grow on P1 and P7 lysogens. Neither P1 nor P7 is able to grow on a D6 lysogen. This is not due to DNA restriction or to the elimination of phage receptor sites on the host's surface. Therefore a nonreciprocal immunity relationship appears to exist between P1–P7 and D6. Mutants of P1 and P7 which are not subject to repression by the P1–P7 c 1+ gene product are able to grow on a D6 lysogen, implying that D6 has a repressor activity like that of P1c 1. There are two regions of strong hybridization between P1 and D6 DNA; one includes the invertible C loop of Pl. Attempts to demonstrate complementation or recombination between P1 or P7 and D6 were not successful, even for markers in the regions of homology.