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DNA Synthesis Initiated At OriC: In Vitro Replication Reactions.

E. Crooke
Published 1995 · Biology, Medicine

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Publisher Summary In vitro replication initiated at the Escherichia coli chromosomal origin (oriC) can occur in a precisely prepared cell extract or it can be reconstituted with purified proteins. The development of these systems was possible following the identification of oriC and the construction of oriC-containing plasmids. In vivo , these plasmids act as minichromosomes in that their replication is initiated synchronously with that of the cell chromosome and with similar requirements. Behaving as such, these plasmids can serve as templates in vitro to study replication at oriC. Another major advance in the development of systems to replicate oriC in vitro is the preparation of an extract containing all of the required factors and lacking inhibitors. Combined with knowledge gained from studying replication in other systems such as bacteriophage φX174, replication in the crude extract permitted the identification, isolation, and characterization of the numerous components necessary to reconstitute oriC DNA replication. Replication in cell extracts and in systems of defined components has many physiological features. It requires the addition of template DNA, which contains the oriC sequence, and replication is initiated at or near oriC. It is dependent on certain replication proteins that are identified as important for chromosomal replication in vivo . Included in these is DnaA, a protein that is implicated genetically as playing a central role in initiating replication at oriC.
This paper references
Experiments in molecular genetics
J. Miller (1972)
10.1016/0092-8674(88)90014-1
Transcriptional activation of initiation of replication from the E. coli chromosomal origin: An RNA-DNA hybrid near oriC
T. A. Baker (1988)
10.1016/0167-4781(88)90155-8
Macromolecular crowding extends the range of conditions under which DNA polymerase is functional.
S. Zimmerman (1988)
10.1016/0092-8674(84)90539-7
Replication initiated at the origin (oriC) of the E. coli chromosome reconstituted with purified enzymes
J. Kaguni (1984)
10.1016/0092-8674(88)90412-6
Duplex opening by dnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome
D. Bramhill (1988)
Complete enzymatic replication of plasmids containing the origin of the Escherichia coli chromosome.
B. Funnell (1986)
10.1002/j.1460-2075.1985.tb03779.x
Importance of state of methylation of oriC GATC sites in initiation of DNA replication in Escherichia coli.
D. W. Smith (1985)
10.1016/0092-8674(86)90537-4
Extensive unwinding of the plasmid template during staged enzymatic initiation of DNA replication from the origin of the Escherichia coli chromosome
T. A. Baker (1986)
Fate of the DnaA initiator protein in replication at the origin of the Escherichia coli chromosome in vitro.
B. Y. Yung (1990)
Sequential early stages in the in vitro initiation of replication at the origin of the Escherichia coli chromosome.
K. Sekimizu (1988)
10.1002/j.1460-2075.1990.tb07406.x
Strand separation required for initiation of replication at the chromosomal origin of E.coli is facilitated by a distant RNA–DNA hybrid.
K. Skarstad (1990)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)



This paper is referenced by
10.1074/jbc.M111.223495
Remodeling of Nucleoprotein Complexes Is Independent of the Nucleotide State of a Mutant AAA+ Protein*
Rahul Saxena (2011)
10.1093/emboj/17.14.4158
Effects of purified SeqA protein on oriC‐dependent DNA replication in vitro
S. Wold (1998)
10.1006/JMBI.2001.4494
Structural biochemistry of a type 2 RNase H: RNA primer recognition and removal during DNA replication.
B. Chapados (2001)
10.1016/j.fob.2013.03.001
Different effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro☆
M. Maciąg-Dorszyńska (2013)
10.1038/sj.emboj.7600990
A novel regulatory mechanism couples deoxyribonucleotide synthesis and DNA replication in Escherichia coli
S. Gon (2006)
10.18388/abp.2001_3860
Cloning of the Haemophilus influenzae Dam methyltransferase and analysis of its relationship to the Dam methyltransferase encoded by the HP1 phage.
J. Bujnicki (2001)
10.1074/JBC.M308516200
Reconstitution of R6K DNA Replication in Vitro Using 22 Purified Proteins*
M. Abhyankar (2003)
10.1093/emboj/20.15.4253
Hda, a novel DnaA‐related protein, regulates the replication cycle in Escherichia coli
J. Kato (2001)
10.1046/j.1365-2958.2001.02345.x
CspD, a novel DNA replication inhibitor induced during the stationary phase in Escherichia coli
K. Yamanaka (2001)
10.1016/S0300-9084(00)01224-4
Escherichia coli DnaA protein--phospholipid interactions: in vitro and in vivo.
E. Crooke (2001)
10.1016/S0092-8674(00)81222-2
The Initiator Function of DnaA Protein Is Negatively Regulated by the Sliding Clamp of the E. coli Chromosomal Replicase
T. Katayama (1998)
10.1006/BBRC.1997.6244
Conformational transition of DnaA protein by ATP: structural analysis of DnaA protein, the initiator of Escherichia coli chromosome replication.
T. Kubota (1997)
10.1128/JB.185.18.5563-5572.2003
A dual binding site for integration host factor and the response regulator CtrA inside the Caulobacter crescentus replication origin.
R. Siam (2003)
10.1006/BBRC.2001.5898
DnaA protein Lys-415 is close to the ATP-binding site: ATP-pyridoxal affinity labeling.
T. Kubota (2001)
10.1074/JBC.M400021200
Reconstitution of F Factor DNA Replication in Vitro with Purified Proteins*
S. Zzaman (2004)
10.1006/PREP.1999.1094
Functional analysis of affinity-purified polyhistidine-tagged DnaA protein.
Z. Li (1999)
10.1074/jbc.M413923200
Restoration of Growth to Acidic Phospholipid-deficient Cells by DnaA(L366K) Is Independent of Its Capacity for Nucleotide Binding and Exchange and Requires DnaA*
Z. Li (2005)
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