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The Localization Of Replication Origins On ARS Plasmids In S. Cerevisiae

B. Brewer, W. L. Fangman
Published 1987 · Biology, Medicine, Geology

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Replication intermediates from the yeast 2 microns plasmid and a recombinant plasmid containing the yeast autonomous replication sequence ARS1 have been analyzed by two-dimensional agarose gel electrophoresis. Plasmid replication proceeds through theta-shaped (Cairns) intermediates, terminating in multiply interlocked catenanes that are resolved during S phase to monomer plasmids. Restriction fragments derived from the Cairns forms contain replication forks and bubbles that behave differently from one another when subjected to high voltage and agarose concentrations. The two-dimensional gel patterns observed for different restriction fragments from these two plasmids indicate that in each plasmid there is a single, specific origin of replication that maps, within the limits of our resolution, to the ARS element. Our results strongly support the long-standing assumption that in Saccharomyces cerevisiae an ARS is an origin of replication.
This paper references
10.1016/0092-8674(87)90643-X
The in vivo replication origin of the yeast 2μm plasmid
J. Huberman (1987)
10.1016/0092-8674(79)90332-5
Replication of each copy of the yeast 2 micron DNA plasmid occurs during the S phase
V. Zakian (1979)
10.1093/NAR/9.24.6805
Separation of closed circular DNA from linear DNA by electrophoresis in two dimensions in agarose gels.
A. Oppenheim (1981)
10.1083/JCB.85.1.108
Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae
C. Rivin (1980)
Replication origins in the eu - caryotic chromosome
R. A.. Laskey (1981)
10.1002/YEA.320010102
The yeast ARS element, six years on: A progress report
D. H. Williamson (1985)
10.1016/0092-8674(83)90069-7
ARS replication during the yeast S phase
W. L. Fangman (1983)
10.1016/0092-8674(81)90316-0
Replication origins in the eucaryotic chromosome
R. Laskey (1981)
10.1016/0092-8674(83)90182-4
Yeast plasmid requires a cis-acting locus and two plasmid proteins for its stable maintenance
Y. Kikuchi (1983)
10.1128/MCB.5.1.85
Method of mapping DNA replication origins.
L. Spotila (1985)
10.1016/0092-8674(75)90172-5
Replicating circular DNA molecules in yeast
T. Petes (1975)
10.1073/PNAS.81.9.2616
DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication.
S. Dinardo (1984)
10.1016/B978-0-12-583580-0.50038-8
REPLICATION ORIGINS USED in vivo IN YEAST
C. Newlon (1981)
10.1177/001088046600700305
The
H. Peretz (1966)
10.1016/0092-8674(83)90139-3
The yeast plasmid 2μ circle encodes components required for its high copy propagation
M. Jayaram (1983)
10.1093/NAR/13.8.2943
DNA synthesis in yeast cell-free extracts dependent on recombinant DNA plasmids purified from Escherichia coli.
A. Jong (1985)
10.1016/0092-8674(80)90118-X
Terminal stages of SV40 DNA replication proceed via multiply intertwined catenated dimers
O. Sundin (1980)
Localization and sequence analysis
J. R. Broach (1983)
10.1016/0378-1119(86)90293-3
A rapid, efficient method for isolating DNA from yeast.
C. Holm (1986)
10.1002/BIES.950040405
Replication origins in yeast chromosomes
S. Kearsey (1986)
10.1016/S0092-8674(85)80028-3
DNA topoisomerase II is required at the time of mitosis in yeast
C. Holm (1985)
10.1073/PNAS.75.5.2170
Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.
D. Shortle (1978)
10.1146/ANNUREV.CB.01.110185.001445
Chromosome segregation in mitosis and meiosis.
A. Murray (1985)
ARS element , six years on : a prog ress report
V. A. Zakian (1979)
10.1101/SQB.1983.047.01.132
Localization and sequence analysis of yeast origins of DNA replication.
J. Broach (1983)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
10.1038/286860A0
Nucleotide sequence of the yeast plasmid
J. Hartley (1980)
10.1016/0003-2697(83)90628-0
Separation of branched from linear DNA by two-dimensional gel electrophoresis.
L. Bell (1983)
10.1073/PNAS.76.7.3445
Occurrence of crossed strand-exchange forms in yeast DNA during meiosis.
L. Bell (1979)
10.1016/0092-8674(85)90153-9
Genetic analysis of the mitotic transmission of minichromosomes
D. Koshland (1985)
10.1016/0092-8674(82)90420-2
Yeast DNA replication in vitro: Initiation and elongation events mimic in vivo processes
S. Celniker (1982)
ARS replication during the yeast
W. L. Fangman (1983)
DNA topoisomerase II is required at the time of mitosis
C. Holm (1985)



This paper is referenced by
Extrachromosomal elements cause a reduced division potential in nib 1 strains of Saccharomyces cerevisiae.
R. Sweeney (1989)
10.1016/0076-6879(90)85024-I
[22] Propagation and expression of cloned genes in yeast: 2-μm circle-based vectors
A. Rose (1990)
10.1016/0092-8674(89)90012-3
The replication terminator protein of E. coli is a DNA sequence-specific contra-helicase
G. S. Khatri (1989)
10.1016/0092-8674(90)90291-L
Reduced DNA polytenization of a minichromosome region undergoing position-effect variegation in Drosophila
Cary H. Karpen (1990)
10.1128/MCB.12.9.4056
Replication forks pause at yeast centromeres.
S. Greenfeder (1992)
DNA Replication in Chloroplasts
S. Heinhorst (1993)
10.1016/0092-8674(94)90173-2
Meiosis-specific formation of joint DNA molecules containing sequences from homologous chromosomes
I. Collins (1994)
10.1016/0968-0004(88)90086-2
Eukaryotic DNA replication: yeast bares its ARSs.
J. Campbell (1988)
10.1128/MCB.17.4.2090
Stability of a CTG/CAG trinucleotide repeat in yeast is dependent on its orientation in the genome.
C. Freudenreich (1997)
10.1128/mBio.00152-19
Interaction of the Human Papillomavirus E1 Helicase with UAF1-USP1 Promotes Unidirectional Theta Replication of Viral Genomes
Marit Orav (2019)
10.1038/embor.2009.67
Chromatin assembly controls replication fork stability
Marta Clemente-Ruiz (2009)
10.1038/sj.emboj.7601155
Tracking of controlled Escherichia coli replication fork stalling and restart at repressor‐bound DNA in vivo
C. Possoz (2006)
10.1128/MCB.19.2.1226
Cyclin B-Cdk1 Kinase Stimulates ORC- and Cdc6-Independent Steps of Semiconservative Plasmid Replication in Yeast Nuclear Extracts
B. Duncker (1999)
10.1016/S0169-4758(98)01222-8
The ribosomal DNA plasmids of entamoeba.
S. Bhattacharya (1998)
10.1007/s002849900414
The Autonomously Replicating Sequence (ARS) of the Yeast Saccharomyces exiguus Yp74L-3
T. Hisatomi (1999)
10.1007/s002940050526
A circular mitochondrial plasmid incites hypovirulence in some strains of Cryphonectria parasitica
C. B. Monteiro-Vitorello (2000)
10.1016/S0968-0004(03)00132-4
The mitochondrial DNA replication bubble has not burst.
D. Bogenhagen (2003)
10.1016/J.MITO.2006.11.023
The role of Pif1p, a DNA helicase in Saccharomyces cerevisiae, in maintaining mitochondrial DNA.
Xin Cheng (2007)
10.1007/978-1-59745-527-5_15
Using Schizosaccharomyces pombe meiosis to analyze DNA recombination intermediates.
Randy W. Hyppa (2009)
10.1074/jbc.273.29.18300
An Initiation Zone of Chromosomal DNA Replication at the Chicken Lysozyme Gene Locus*
L. Phi-Van (1998)
10.5772/20383
Eukaryotic Replication Barriers: How, Why and Where Forks Stall
J. Dalgaard (2011)
The smc5/6 complex : linking DNA replication with chromosome segregation
Kristian Jeppsson (2015)
10.1371/journal.pgen.1001328
Identification of a Novel Type of Spacer Element Required for Imprinting in Fission Yeast
Suha Sayrac (2011)
10.1016/j.febslet.2004.12.011
Origin recognition and the chromosome cycle
B. Stillman (2005)
Characterization of the DNA Damage Resistance Gene RTT107
T. Roberts (2008)
Cartographie pangénomique à haut débit et en molécule unique de la réplication de l'ADN
Nikita Menezes braganca (2019)
10.1534/genetics.119.302351
Phenotypic and Genotypic Consequences of CRISPR/Cas9 Editing of the Replication Origins in the rDNA of Saccharomyces cerevisiae
J. C. Sanchez (2019)
10.1007/978-3-642-76988-7_11
Mutations Affecting Replication Origin Function in Yeast
S. Kearsey (1992)
10.1016/0006-291X(89)91571-4
Unusual DNA structures at the integration site of an HIV provirus.
M. A. Gama Sosa (1989)
10.1016/0167-4781(91)90051-M
Cell-type-specific transcription in yeast.
Joseph W. Dolan (1991)
10.1007/BF00356030
The replication behavior of Saccharomyces cerevisiae DNA in human cells
Chi T. Tran (2004)
10.1016/0959-437X(93)90110-B
Mammalian chromosome structure.
C. Tyler-Smith (1993)
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