Online citations, reference lists, and bibliographies.
← Back to Search

Degradation Of Fully Water-soluble, Partially N-acetylated Chitosans With Lysozyme

Ragnhild J. Nordtveit, K. M. Vårum, O. Smidsrød
Published 1994 · Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Chitosans, prepared by homogeneous N-deacetylation of chitin, with degrees of N-acetylation ranging from 4 to 60% (FA = 0·04 to 0·60) exhibiting full water solubility and known random distribution of acetyl groups, were degraded with lysozyme. Initial degradation rates (r) were determined from plots of the viscosity decrease (Δ1/[η]) against time of degradation. The time course of degradation of chitosans with lysozyme were non-linear, while the time course of degradation of chitosans with an oxidative-reductive depolymerization reaction (using H2O2) showed the expected linear relationship for a first-order, random depolymerization reaction, independent of the chemical composition of the chitosan. The effect of lysozyme concentration and substrate concentration on the initial degradation rates were determined, showing that this lysozyme-chitosan system obeys Michaelis-Menten kinetics. The initial degradation rates of chitosan with lysozyme increased strongly with increasing fraction of acetylated units (FA). From a Michaelis-Menten analysis of the degradation data that assumes different catalytic activities of lysozyme for the different hexameric substrates in the polysaccharide chain, it is concluded that the hexameric substrates that contain three-four or more acetylated units contribute mostly to the initial degradation rate when lysozyme degrades partially N-acetylated chitosans. A chitosan with a very low fraction of acetylated units (FA = 0·010) was studied as an enzyme inhibitor. Initial degradation rates of chitosan (with different FA values) decreased as the inhibitor concentration increased, while the relative rates stayed constant, indicating that the ratio between initial reaction rates for productive sites (hexamers containing three-four or more N-acetylated units) are unaffected by non-productive sites, as deduced from the theory of competing substrates.
This paper references
Studies on chitin, 2. Effect of deacetylation on solubility
T. Sannan (1976)
Lysozyme susceptibility of partially deacetylated chitin.
H. Sashiwa (1990)
The action of lysozyme on partially deacetylated chitin.
Ken-ichi Amano (1978)
On the enzymatic hydrolysis of carboxymethylchitin by lysozyme.
A. Marzotto (1969)
13C-n.m.r. studies of the acetylation sequences in partially N-deacetylated chitins (chitosans).
K. M. Vårum (1991)
Studies on chitin: 7. I.r. spectroscopic determination of degree of deacetylation
T. Sannan (1978)
Kinetic Studies on the Degradation of Alginic Acid by Hydrogen Peroxide in the Presence of Iron Salts.
O. Smidsrød (1965)
Determination of the Mark-Houwink equation for chitosans with different degrees of deacetylation.
W. Wang (1991)
Solution properties of chitosans: conformation and chain stiffness of chitosans with different degrees of N-acetylation
M. Anthonsen (1993)
Physical Chemistry of Macromolecules
C. Tanford (1961)
N-acetylation in chitosan and the rate of its enzymic hydrolysis.
S. Hirano (1989)
1H-NMR study on the chitotrisaccharide binding to hen egg white lysozyme.
T. Fukamizo (1992)
Enzyme structure and mechanism
A. Fersht (1977)
Determination of the degree of N-acetylation and the distribution of N-acetyl groups in partially N-deacetylated chitins (chitosans) by high-field n.m.r. spectroscopy.
K. M. Vårum (1991)
Oxidative-reductive depolymerization: a note on the comparison of degradation rates of different polymers by viscosity measurements
O. Smidsrød (1967)
Chitosan cross-linked with Mo(VI) polyoxyanions: a new gelling system.
K. Draget (1992)

This paper is referenced by
Chitosan Nanoparticles as New Ocular Drug Delivery Systems: in Vitro Stability, in Vivo Fate, and Cellular Toxicity
A. M. de Campos (2004)
Comparison of self-aggregated chitosan particles prepared with and without ultrasonication pretreatment as Pickering emulsifier
Kiang Wei Ho (2016)
Chitosan nanoparticles as a new delivery system for the chemotherapy agent tegafur
J. L. Arias (2010)
2.13 Chitosan
M. Barbosa (2017)
Chitosans as absorption enhancers of poorly absorbable drugs. 3: Influence of mucus on absorption enhancement.
N. G. Schipper (1999)
Biomedical Applications of Chitin and Chitosan Derivatives
Sougata Jana (2013)
Fe3O4/chitosan nanocomposite for magnetic drug targeting to cancer
J. L. Arias (2012)
Progress in antimicrobial activities of chitin, chitosan and its oligosaccharides: a systematic study needs for food applications
J. Dutta (2012)
The potential of chitosan in ocular drug delivery
M. Alonso (2003)
Process characteristics of hydrolysis of chitosan in a continuous enzymatic membrane reactor
C. Kuo (2006)
Biomedical Application of Nanofiber
Jirapun Paraboon (2010)
Production and Characterisation of Self-Crosslinked Chitosan-Carrageenan Polyelectrolyte Complexes
Nawar Al-Zebari (2017)
Influence of glucosamine on oligochitosan solubility and antibacterial activity.
I. Blagodatskikh (2013)
14 – Biopolymers for wood preservation
S. Patachia (2016)
Competitive binding of highly de-N-acetylated chitosans and N,N'-diacetylchitobiose to lysozyme from chicken egg white studied by 1H NMR spectroscopy.
A. Kristiansen (1996)
In vitro degradation of chitosan by bacterial enzymes from rat cecal and colonic contents.
H. Zhang (2002)
N-carboxyethyl chitosan fibers prepared as potential use in tissue engineering.
Shuoshuo Yang (2016)
Recent advances in the synthesis of chitooligosaccharides and congeners
Y. Yang (2014)
Mode of action of chitin deacetylase from Mucor rouxii on partially N-acetylated chitosans
A. Martinou (1998)
Development of alpha-lipoic acid encapsulated chitosan monodispersed particles using an electrospray system: synthesis, characterisations and anti-inflammatory evaluations
Meng-Yi Bai (2014)
In Vitro Enzymatic Digestibility of Glutaraldehyde-Crosslinked Chitosan Nanoparticles in Lysozyme Solution and Their Applicability in Pulmonary Drug Delivery
N. Islam (2019)
Preparation of Chito-Oligomers by Hydrolysis of Chitosan in the Presence of Zeolite as Adsorbent
K. Ibrahim (2016)
Rat sciatic nerve regeneration across a 10-mm defect bridged by a chitin/CM-chitosan artificial nerve graft.
Z. Jiang (2019)
The enzymatic degradation and swelling properties of chitosan matrices with different degrees of N-acetylation.
Dongwen Ren (2005)
Chitosan/poly(vinyl alcohol) hydrogels for amoxicillin release
Aylin Altınışık (2013)
Evaluación del uso de quitina y rhBMP-2 para la regeneración ósea: una aproximación desde la Ingeniería de Tejidos
C. D. López (2017)
Immunological responses to chitosan for biomedical applications
C. D. Hoemann (2017)
Polysaccharides Route: A New Green Strategy for Metal Oxides Synthesis
Diana Visinescu (2012)
Controlling cell adhesion and degradation of chitosan films by N-acetylation.
T. Freier (2005)
Diet supplement based on radiation-modified chitosan and radiation-synthesized polyvinylpyrrolidone microgels: Influence on the liver weight in rats fed a fat- and cholesterol-rich diet†
Renata Czechowska-Biskup (2007)
Binding of a Highly De-IV-acetylated Lysozyme as Measured by iH-NMRChitosan to SpectroscopyJapanese Pheasan
T. Fukamizo (2018)
Degradation properties and metabolic activity of alginate and chitosan polyelectrolytes for drug delivery and tissue engineering applications
V. Guarino (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar