Online citations, reference lists, and bibliographies.
← Back to Search

Identification Of A Polyadenylated Transcript Correlated With Development In The Sexual Cycle Of Schizophyllum

C. Raper, W. Timberlake
Published 1985 · Biology

Save to my Library
Download PDF
Analyze on Scholarcy
Abstract A polyadenylated transcript of about 580 nucleotides has been determined to occur preferentially in the dikaryon of Schizophyllum commune. It was apparent in blots of RNA from the vegetative dikaryon (sexually differentiated cells which are normally capable of developing into fruiting bodies) and not apparent in blots of RNA from the two monokaryotic homokaryons (sexually nondifferentiated cells) that were mated to obtain the dikaryon. It was detected by probing gel blots of poly(A)+ RNA from the dikaryon and from each of the two component unmated monokaryons simultaneously with a subset of cDNAs specific to the dikaryon. The “dikaryon-specific” cDNAs were obtained by isolating poly(A)+ RNA from the dikaryon prior to fruiting, preparing 32P-labeled cDNA from this RNA, and selecting those cDNA sequences that did not hybridize to the poly(A)+ RNA of the two component monokaryons. A probe consisting of dikaryotic cDNA that did hybridize to the poly(A)+ RNA of the monokaryons served as the control. A relatively small degree of gene regulation at the transcriptional level appears to be involved in differentiation of the dikaryon from the component monokaryons. A series of successive hybridizations (“cascade hybridization”) of 32P-labeled cDNA from the dikaryon with poly(A)+ RNA from the monokaryons revealed only 2% of the starting material as noncomplementary to the RNA of the monokaryons. This is in marked contrast to the high degree of transcriptional regulation observed for developmental processes in other fungi such as Aspergillus nidulans.
This paper references
A Class of Genes Affecting B Factor-Regulated Development in SCHIZOPHYLLUM COMMUNE.
C. Dubovoy (1976)
Molecular cloning of a gene abundantly expressed during fruiting body initiation in Schizophyllum commune.
J. Dons (1984)
Sequence analysis of a split gene involved in fruiting from the fungus Schizophyllum commune
J. Dons (1984)
Regulation of the pattern of protein synthesis in Schizophyllum commune by the incompatibility genes.
O. D. de Vries (1980)
Analysis of repeating DNA sequences by reassociation.
R. Britten (1974)
Relationship between nuclear and polysomal RNA populations of Achlya: a simple eucaryotic system
W. Timberlake (1977)
Diversity and abundance of polyadenylated RNA from Achlya ambisexualis.
C. Rozek (1978)
Alterations in RNA and protein synthesis associated with steroid hormone-induced sexual morphogenesis in the water mold Achlya.
W. Timberlake (1976)
Gene expression during development of Blastocladiella emersonii
S. Johnson (1984)
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
J. Bailey (1976)
Developmental gene regulation in Aspergillus nidulans.
W. Timberlake (1980)
Absence of evidence for changes in messenger RNA populations during steroid hormone-induced cell differentiation inAchlya
C. Rozek (1980)
Changes in polyadenylated RNA sequences associated with fruiting body morphogenesis in Coprinus cinereus
B. Yashar (1985)
The Genetics of Schizophyllum Commune.
J. Raper (1958)
Jhc Hoge (1982)
Frequency and diversity of RNA sequences in different cell types of the fungus Schizophyllum commune.
B. Zantinge (1981)
Methods in Enzymology , Vol
S. Colowick (1966)
Mutations modifying sexual morphogenesis in schizophyllum.
C. Raper (1966)
RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
H. Lehrach (1977)
Relationships between mitochondria and longevity determinants in the ascomycetePodospora anserina
A. Raynal (1980)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar