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Analytical Applications Of Immobilised Proteins And Cells

M. Coughlan, M. Kierstan, P. Border, A. Turner
Published 1988 · Chemistry

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I n t roduc t i on . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 I m m o b i l i s a t i o n of prote ins and cells: the concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Genera l app l i ca t ions of immobi l i s ed prote ins and cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Ana ly t i ca l uses of immobi l i s ed prote ins and cells: an in t roduc t ion . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 C lass i f i ca t ion of ana ly t ica l devices i nco rpora t ing immobi l i s ed b i o c o m p o n e n t s . . . . . . . . . . . . . . . . . 3 (i) Misce l laneous ana ly t ica l devices based on immobi l i s ed b i o c o m p o n e n t s . . . . . . . . . . . . . . . . 3 (ii) Immob i l i s ed enzyme reactors in a u t o m a t e d analys is . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 (iii) Biosensors: an i n t roduc t i on . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Procedures used to immobi l i s e the b io log ica l c o m p o n e n t of b iosensors . . . . . . . . . . . . . . . . . . . . . . . 9 E n t r a p m e n t and encapsu la t ion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Cova len t i m m o b i l i s a t i o n . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 Direct versus indi rec t immob i l i s a t i on . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 The role of t r ansducers in b iosensor cons t ruc t ion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 E lec t rochemica l t ransducers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 Po ten t iomet r i c e lect rodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 A m p e r o m e t r i c e lectrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 C o n d u c t i m e t r i c e lectrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 Opt ica l t ransducers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 Ca lo r ime t r i c t r ansducers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Piezoelect r ic and o ther t ransducers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 App l i ca t i ons of b iosensors : versatil i ty, advan tages and d i sadvan tages . . . . . . . . . . . . . . . . . . . . . . . . . 23 Genera l aspects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Advan tages and d i sadvan tages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 S ter i l i sa t ion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 Stabi l i ty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 Medico-c l in ica l app l i ca t ions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 App l i ca t i ons in the food, d r ink and dai ry indus t r ies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Indus t r i a l b ioreac tor and fe rmen ta t ion cont ro l . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 E n v i r o n m e n t a l m o n i t o r i n g . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
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