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Monitoring Sulfate-reducing Bacteria: Comparison Of Enumeration Media

R. S. Tanner
Published 1989 · Biology

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Abstract The standard medium (API Recommended Practice RP-38) for the enumeration of sulfate-reducing bacteria (SRB) in oil-field waters was modified by the addition of an ammonium salt, a Ca salt, vitamins, trace metals and an additional reductant, cysteine. The modified medium was compared to API RP-38 medium, Postgate's medium B a modified Baar's medium. While the enumeration of Desulfovibrio desulfuricans and Desulfovibrio vulgaris was not influenced by the medium used, the enumeration of SRB from environmental samples did depend on the choice of medium. Higher counts of SRB were obtained from environmental samples in a shorter time with the modified compared to the standard API RP-38 medium. Enumeration of SRB was also generally more rapid with the modified medium compared to Postgate's medium B and the modified Baar's medium
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