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Induction Of Ornithine Decarboxylase In Adult Rat Hippocampal Slices.

M. Baudry, K. Shahi, C. Gall
Published 1988 · Biology, Medicine

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Several factors involved in the regulation of ornithine decarboxylase (ODC) activity in adult rat brain tissue have been identified by using the in vitro hippocampal slice preparation. The same amino acids that have previously been reported to induce ODC in tissue culture, i.e., asparagine and glutamine, were found to produce a concentration- and time-dependent increase in ODC activity that reached a 100 fold the control value after 6 h of incubation. The effect of asparagine was totally blocked by inhibition of either protein or RNA synthesis, suggesting that the inducing amino acids increase ODC activity by stimulating the transcription of genes directly or indirectly regulating ODC activity. The effect of the inducing amino acids was potentiated by a variety of factors which by themselves did not modify ODC activity. In particular, opioid peptides markedly potentiated the effect of asparagine. Although the opiate antagonists naloxone and naltrexone totally blocked the effects of the opioid peptides on ODC induction, they also produced an inhibition of the asparagine-mediated increase in ODC activity. Other factors like dibutyryl cyclic AMP and insulin also potentiated the effects of asparagine on ODC activity. These results provide the first description of ODC induction in an in vitro preparation of adult brain tissue and indicate that the hippocampal slice preparation could be used to study the molecular mechanisms which regulate the expression and activity of ODC in the adult central nervous system. Moreover the data suggest possible mechanisms which may be involved in the induction of ODC in hippocampus by seizure activity.
This paper references
10.1016/0014-2999(83)90233-9
Regulation by calcium ions of glutamate receptor binding in hippocampal slices.
M. Baudry (1983)
10.1016/0024-3205(83)90862-7
Stimulation of ornithine decarboxylase by histamine or norepinephrine in brain regions of the developing rat: evidence for biogenic amines as trophic agents in neonatal brain development.
G. Morris (1983)
10.1016/0092-8674(87)90557-5
Growth signal transduction: Rapid activation of covalently bound ornithine decarboxylase during phosphatidylinositol breakdown
T. Mustelin (1987)
10.1523/JNEUROSCI.06-12-03430.1986
Induction of ornithine decarboxylase as a possible mediator of seizure- elicited changes in genomic expression in rat hippocampus
M. Baudry (1986)
10.1007/BF01141022
Epidermal growth factor
S. Cohen (1987)
10.1016/0006-8993(80)90180-8
Release of amino acids from chronic epileptic and sub-epileptic foci in vivo
P. Dodd (1980)
10.1038/315059A0
Co-localization of corticotropin releasing factor and vasopressin mRNA in neurones after adrenalectomy
B. Wolfson (1985)
N alpha-acetyl-beta-endorphin stimulates ornithine decarboxylase activity in preweanling rat pups: opioid- and non-opioid-mediated mechanisms.
J. Bartolome (1987)
10.1111/j.1471-4159.1984.tb09689.x
Glutamate: A Neurotransmitter in Mammalian Brain
F. Fonnum (1984)
10.1016/0024-3205(86)90643-0
Effects of beta-endorphin on ornithine decarboxylase in tissues of developing rats: a potential role for this endogenous neuropeptide in the modulation of tissue growth.
J. Bartolome (1986)
10.1038/276191A0
Induction of ornithine decarboxylase by nerve growth factor dissociated from effects on survival and neurite outgrowth
L. Greene (1978)
10.1073/PNAS.74.9.3791
Enzyme regulation in neuroblastoma cells in a salts/glucose medium: induction of ornithine decarboxylase by asparagine and glutamine.
K. Chen (1977)
10.1126/SCIENCE.6612331
Increased brain size and cellular content in infant rats treated with an opiate antagonist.
I. Zagon (1983)
10.1073/PNAS.82.13.4365
Induction of ornithine decarboxylase activity by insulin and growth factors is mediated by amino acids.
C. Rinehart (1985)
10.1073/PNAS.75.2.1021
Nerve growth factor increases activity of ornithine decarboxylase in rat brain.
M. Lewis (1978)
10.1016/s0021-9258(18)47866-0
Molecular mechanisms of the synergistic induction of ornithine decarboxylase by asparagine and glucagon in primary cultured hepatocytes.
R. Kanamoto (1987)
10.1016/0024-3205(76)90427-6
Proposed model of major sequential biochemical events of a trophic response.
D. H. Russell (1976)
10.1523/JNEUROSCI.06-10-03094.1986
Kainic acid alters the metabolism of Met5-enkephalin and the level of dynorphin A in the rat hippocampus
T. Kanamatsu (1986)
10.1002/JCP.1041230321
The effect of transport system A and N amino acids and of nerve and epidermal growth factors on the induction of ornithine decarboxylase activity
C. Rinehart (1985)
10.1037/e496622006-002
Localization and seizure-induced alterations of opioid peptides and CCK in the hippocampus.
C. Gall (1988)
10.1111/J.1432-0436.1981.TB01123.X
Role of polyamines in the control of cell proliferation and differentiation.
O. Heby (1981)
10.1016/0196-9781(88)90228-8
Focal hippocampal lesions induce seizures and long-lasting changes in mossy fiber enkephalin and CCK immunoreactivity
C. Gall (1988)
10.1002/JCP.1041070206
Cellular control of ornithine decarboxylase activity by its antizyme
J. Heller (1981)
10.1126/SCIENCE.6147894
Neurotransmitter plasticity at the molecular level.
I. Black (1984)
10.1111/j.1471-4159.1984.tb12710.x
Induction of Brain Ornithine Decarboxylase During Recovery from Metabolic, Mechanical, Thermal, or Chemical Injury
G. Dienel (1984)
10.1146/ANNUREV.NE.03.030180.000245
The use of in vitro brain slices for multidisciplinary studies of synaptic function.
G. Lynch (1980)
10.1038/285231A0
Changes of hippocampal Met-enkephalin content after recurrent motor seizures
J. Hong (1980)
10.1523/JNEUROSCI.07-03-00753.1987
Enkephalin biosynthesis and enkephalin gene expression are increased in hippocampal mossy fibers following a unilateral lesion of the hilus
J. White (1987)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1016/0006-8993(86)90738-9
Changes in dynorphin, enkephalin and cholecystokinin content of hippocampus and substantia nigra after amygdala kindling
M. Iadarola (1986)
10.1111/j.1471-4159.1985.tb07184.x
Ornithine Decarboxylase Activity in Brain Regulated by a Specific Macromolecul, the Antizyme
Päivi H. Laitinen (1985)



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