Online citations, reference lists, and bibliographies.

Formalin Fixation Effects On Vertebral Bone Density And Failure Mechanics: An In-vitro Study Of Human And Sheep Vertebrae.

S. Edmondston, K. Singer, R. Day, P. Breidahl, R. Price
Published 1994 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Vertebral bone density of two human vertebral specimens was evaluated using dual-anergy X-ray absorptiometry and single-energy quantitative computed tomography immediately before and after 4 weeks of fixation. The repeated QCT assessment of these segments produced a mean rate of change of 1.17 HU/month, while for DXA the mean rate of change was -0.002 g/month for BMC and -0.0004 g/cm(2)/month for BMD. Ten fresh and 10 fixed sheep lumbar spines underwent absorptiometry before being sectioned into units comprising a central vertebra and adjacent intervertebral discs, plus 1 cm of the flanking vertebral end-plate for embedding into bone cement. The fixed sheep spines underwent a repeat scan after 4 weeks in 10% formalin. Mechanical testing produced a significantly lower average failure load for unfixed specimens (9.3 kN) than for the fixed material (10.8 kN). Failure strain was not significantly different between groups. Linear regression showed a high correlation of BMC values before and after 4 weeks of formalin fixation, while the slopes of the regression for BMD and failure load of both fresh and fixed groups were not significantly different. While formalin fixation may result in a slight increase in compressive strength this does not appear to be associated with a systematic change in mineral density.



This paper is referenced by
10.1016/J.BONE.2004.04.008
Determinants and heterogeneity of mechanical competence throughout the thoracolumbar spine of elderly women and men.
F. Eckstein (2004)
10.1016/j.jmbbm.2013.09.008
Ethanol and formaldehyde fixation irreversibly alter bones' organic matrix.
N. Hammer (2014)
10.1016/j.spinee.2016.06.015
First-time systematic postoperative clinical assessment of a minimally invasive approach for lumbar ventrolateral vertebroplasty in the large animal model sheep.
Matthias Bungartz (2016)
10.3233/THC-2003-11303
Subject-specific compressive tolerance estimates.
K. Davis (2003)
10.1016/j.jocd.2010.01.120
Novel assessment of subregional bone mineral density using DXA and pQCT and subregional microarchitecture using micro-CT in whole human vertebrae: applications, methods, and correspondence between technologies.
Andrew M Briggs (2010)
10.1016/j.jbiomech.2008.05.017
An accurate estimation of bone density improves the accuracy of subject-specific finite element models.
E. Schileo (2008)
10.1016/j.clinbiomech.2008.07.007
The effects of embalming using a 4% formalin solution on the compressive mechanical properties of human cortical bone.
C. Ohman (2008)
10.1385/JCD:7:2:169
Multislice computed tomography of the distal radius metaphysis: relationship of cortical bone structure with gender, age, osteoporotic status, and mechanical competence.
H. Bonél (2004)
10.1007/s002230001192
Effect of Fixation, Soft-Tissues, and Scan Projection on Bone Mineral Measurements with Dual Energy X-ray Absorptiometry (DXA)
E. Lochmüller (2001)
10.1007/s002560050576
Precision and intersite correlation of bone densitometry at the radius, tibia and femur with peripheral quantitative CT
O. Groll (1999)
10.1016/J.SPINEE.2005.08.015
Biomechanical evaluation of pedicle screws versus pedicle and laminar hooks in the thoracic spine.
A. Cordista (2006)
10.1515/cdbme-2015-0083
Influence of short-term fixation with mixed formalin or ethanol solution on the mechanical properties of human cortical bone
Enrico Mick (2015)
Vergleich von Vertebroplastie und Kyphoplastie hinsichtlich Versagenslast, Versagensspannung und Lokalisation von Anschlussfrakturen - Eine biomechanische Ex-situ-Untersuchung an Wirbelsäulensegmenten des thorakolumbalen Übergangs mit verminderter Knochendichte
Ludwig-Maximilians-Universitaet Muenchen (2013)
10.1007/s002239900681
Determination of Calcaneal Ultrasound Properties Ex Situ: Reproducibility, Effects of Storage, Formalin Fixation, Maceration, and Changes in Anatomic Measurement Site
G. Pöpperl (1999)
10.1016/j.bone.2012.03.002
Failure strength of human vertebrae: prediction using bone mineral density measured by DXA and bone volume by micro-CT.
E. Perilli (2012)
10.1016/j.jbiomech.2010.08.032
Volume to density relation in adult human bone tissue.
Simone Tassani (2011)
10.5625/lar.2013.29.3.138
Morphometrical dimensions of the sheep thoracolumbar vertebrae as seen on digitised CT images
Mahmoud Mageed (2013)
10.1007/s001980050104
Correlation of Femoral and Lumbar DXA and Calcaneal Ultrasound, Measured In Situ with Intact Soft Tissues, with the In Vitro Failure Loads of the Proximal Femur
E. Lochmüller (1998)
10.1016/S8756-3282(01)00621-4
Mechanical strength of the proximal femur as predicted from geometric and densitometric bone properties at the lower limb versus the distal radius.
E. Lochmüller (2002)
10.1002/JBMR.5650110921
Prediction of fracture load at different skeletal sites by geometric properties of the cortical shell
P. Augat (1996)
10.1007/8415_2011_102
Subject-Specific Models of the Spine for the Analysis of Vertebroplasty
Alison C. Jones (2011)
10.4081/or.2013.e16
Mechanical torque measurement in the proximal femur correlates to failure load and bone mineral density ex vivo
Stefan Grote (2013)
10.1016/j.clinbiomech.2018.09.013
High correlation between mechanical properties and bone mineral parameters in embalmed femurs after long‐term storage
Bryan Wright (2018)
10.1016/j.spinee.2019.11.015
Effect of the intervertebral disc on vertebral bone strength prediction: a Finite-Element Study.
D. Anitha (2019)
10.1016/j.medengphy.2013.04.008
DXA predictions of human femoral mechanical properties depend on the load configuration.
E. Dallara (2013)
10.1016/j.jmbbm.2011.05.032
Study of the behavior of the trabecular bone under cyclic compression with stepwise increasing amplitude.
Tomasz Topoliński (2011)
10.1007/s00774-011-0307-3
Measurement of subregional vertebral bone mineral density in vitro using lateral projection dual-energy X-ray absorptiometry: validation with peripheral quantitative computed tomography
Andrew M Briggs (2011)
10.1007/S007760170023
Mechanical augmentation of the vertebral body by calcium phosphate cement injection
M. Ikeuchi (2001)
10.1016/S8756-3282(98)00127-6
Prediction of vertebral failure loads from spinal and femoral dual-energy X-ray absorptiometry, and calcaneal ultrasound: an in situ analysis with intact soft tissues.
E. Lochmüller (1998)
Einfluss von Konservierung auf die biomechanische Kompetenz von Knochen im Mausmodell
Birthe Anderssohn (2011)
10.1097/BSD.0b013e318030d2d6
Comparative Biomechanical Analysis of an Improved Novel Pedicle Screw With Sheath and Bone Cement
T. Takigawa (2007)
10.5277/ABB-00967-2017-03
The effect of trabecular bone storage method on its elastic properties.
A. Mazurkiewicz (2018)
See more
Semantic Scholar Logo Some data provided by SemanticScholar