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Rapid And Sensitive Detection Of Point Mutations And DNA Polymorphisms Using The Polymerase Chain Reaction.
M. Orita, Y. Suzuki, T. Sekiya, K. Hayashi
Published 1989 · Biology, Medicine
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We report a rapid and sensitive method for the detection of base changes in given sequences of genomic DNA. This technique is based on the facts that specific regions of genomic sequences can be efficently labeled and amplified simultaneously by using labeled substrates in the polymerase chain reaction and that in nondenaturing polyacrylamide gels, the electrophoretic mobility of single-stranded nucleic acid depends not only on its size but also on its sequence. The process does not involve restriction enzyme digestion, blotting, or hybridization to probes. We found that most single base changes in up to 200-base fragments could be detected as mobility shifts. RAS oncogene activation was detected by this technique. We also show that the interspersed repetitive sequences of human, Alu repeats are highly polymorphic.
This paper references
The use of thin acrylamide gels for DNA sequencing
F. Sanger (1978)
Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.
B. J. Conner (1983)
Detection of single base substitutions in total genomic DNA
R. Myers (1985)
Use of labeled primers in polymerase chain reaction (LP-PCR) for a rapid detection of the product.
K. Hayashi (1989)
Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutations
Daniel J. Capon (1983)
Construction of a genetic linkage map in man using restriction fragment length polymorphisms.
D. Botstein (1980)
Base sequence studies of 300 nucleotide renatured repeated human DNA clones.
P. Deininger (1981)
Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
U. Gyllensten (1988)
A method to detect and characterize point mutations in transcribed genes: amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells.
E. Winter (1985)
Sequence of the cDNA and gene for angiogenin, a human angiogenesis factor.
K. Kurachi (1985)
Isolation and regional localization of DNA segments revealing polymorphic loci from human chromosome 13.
W. Cavenee (1984)
A gene deletion ending within a complex array of repeated sequences 3' to the human beta-globin gene cluster.
P. Henthorn (1986)
A general method for isolation of high molecular weight DNA from eukaryotes.
N. Blin (1976)
Mechanism of activation of an N‐ras gene in the human fibrosarcoma cell line HT1080.
R. Brown (1984)
Four human carcinoma cell lines with novel mutations in position 12 of c-K-ras oncogene.
D. Valenzuela (1986)
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
R. Saiki (1988)
A ligase-mediated gene detection technique.
U. Landegren (1988)
Complete sequence and structure of the gene for human adenosine deaminase.
D. Wiginton (1986)
Three different mutations in codon 61 of the human N-ras gene detected by synthetic oligonucleotide hybridization.
J. Bos (1984)
Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.
M. Orita (1989)
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