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Co-cultures Of Human Intestinal Goblet (HT29-H) And Absorptive (Caco-2) Cells For Studies Of Drug And Peptide Absorption

A. Wikman-Larhed, P. Artursson
Published 1995 · Chemistry

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Abstract A co-culture model of the two most abundant intestinal epithelial cell types (absorptive and goblet cells) was developed on permeable supports using the human Caco-2 and HT29-H cell lines. Seeding conditions were selected to give a proportion of goblet cells comparable to the 25–55% found in human colon. The co-cultures comprised small clusters of HT29-H goblet cells embedded in Caco-2 absorptive cells. Electron microscopy showed that tight junctions formed between the two cell types in co-culture. Mono-cultures of HT29-H goblet cells were more permeable to ions and hydrophilic marker molecules and peptides and had a lower Isc than mono-cultures of Caco-2 absorptive cells. The permeability of the co-cultures was intermediate between those of the two mono-cultures. The co-cultures provide a model where absorptive and goblet cells can be studied simultaneously in studies on e.g. drug binding and transport, as well as in studies on absorption enhancement.
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