← Back to Search
Gene Probe Assays On A Fibre-optic Evanescent Wave Biosensor.
C. R. Graham, D. Leslie, D. Squirrell
Published 1992 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
This report describes experiments to detect oligonucleotide hybridization at the surface of a fibre-optic evanescent wave biosensor. Conditions were optimized and the time course of hybridization reactions were found to be very rapid compared to conventional hybridization assays. Binding was easily reversed by heating and the sensor surface could be reused many times. Short (16- and 20-mer) oligonucleotides bound to the waveguide surface could be used to detect fluorescein-labelled complementary sequences at the nanomolar level. Polymerase chain reaction was used to generate a 204-base oligomer that was attached to the waveguide surface. Fluorescein-labelled oligos bound at either the proximal or distal ends of this probe were found to give similar outputs.
This paper references
Effect of numerical aperture on signal level in cylindrical waveguide evanescent fluorosensors.
Thomas R. Glass (1987)
Immunoassays at a quartz-liquid interface: theory, instrumentation and preliminary application to the fluorescent immunoassay of human immunoglobulin G.
R. Sutherland (1984)
Interaction of antibodies with Fc receptors in substrate-supported planar membranes measured by total internal reflection fluorescence microscopy.
C. L. Poglitsch (1990)
Practice and theory of enzyme immunoassays
P. Tijssen (1985)
Pharmacological specificity of a nicotinic acetylcholine receptor optical sensor.
K. R. Rogers (1991)
Instrumentation for Cylindrical Waveguide Evanescent Fluorosensors
Steve Lackie (1991)
Adsorption behavior of fibronectin on well-characterized silica surfaces
U. Jonsson (1982)
Immunoassays and nucleic acid detection with a biosensor based on surface plasmon resonance.
D. Pollard-Knight (1990)
This paper is referenced by
Biosensors in clinical chemistry: An overview
Sathish Babu Murugaiyan (2014)
Biosensors for detecting pathogenic bacteria in the meat industry
E. Alocilja (2008)
Development of a multiarray biosensor for DNA diagnostics
T. Vo-Dinh (1998)
The application of ultrasound as a rapid method to provide DNA fragments suitable for detection by DNA biosensors.
T. Mann (2004)
Microarray and Fluidic Chip for Extracellular Sensing
M. Ozkan (2006)
Comparison study of two aldehyde group finishing agents in surface modification of up-conversion luminescence materials
L. Cui (2008)
N. Sojic (2013)
Chapter 2 – Evanescent Wave Fiber Optic Biosensors
C. Taitt (2002)
Chapter 2 – EVANESCENT WAVE FIBER OPTIC BIOSENSORS
G. Anderson (2008)
A Chemiluminescence Fiber-Optic Biosensor for Detection of DNA Hybridization
Guojun Zhang (1999)
A DNA piezoelectric biosensor assay coupled with a polymerase chain reaction for bacterial toxicity determination in environmental samples
S. Tombelli (2000)
A fiber-optic DNA biosensor microarray for the analysis of gene expression
Jane A. Ferguson (1996)
Nucleic acid based sensors.
F. Bier (1997)
Visualization of DNA hybridization on gold thin film by utilizing the resistance effect of DNA monol
T. Masuda (2005)
Resonant Detection of Nano to Microscopic Objects Using Whispering Gallery Modes
F. Vollmer (2004)
Visual detection of bacterial and viral pathogens with peroxidase-like deoxyribozymes
Daria A. Gorbenko (2020)
Highly Sensitive Probe for Gene Analysis by Electrochemical Approach
S. Takenaka (2001)
EVANESCENT WAVE FIBER OPTIC BIOSENSORS
F. Ligler (1992)
Chitosan scaffolds for biomolecular assembly: Coupling nucleic acid probes for detecting hybridization
H. Yi (2003)
Resolution-improved in situ DNA hybridization detection based on microwave photonic interrogation.
Yuan Cao (2015)
Bioreporters, biosensors, and microprobes.
R. S. Burlage (2007)
Real-time monitoring of DNA hybridization for rapid detection of Vibrio cholerae O1
Nitaya Indrawattana (2014)
Michael Schäferling (2011)
Bioreporters and Biosensors for Environmental Analysis
R. Burlage (1997)
Improved methods for the detection of Bacillus anthracis spores by the polymerase chain reaction
M. Johns (1994)
Fiberoptic DNA sensor array capable of detecting point mutations.
B. Healey (1997)
Bioprocess monitoring and computer control: Key roots of the current PAT initiative
B. Junker (2006)
Genosensor technology and the detention of interfacial nucleic acid chemistry
M. Yang (1997)
Sensing Biological and Chemical Threat Agents
Gopal Coimbatore (2008)
Label-Free Microbial Biosensors Using Molecular Nanowire Transducers
E. Alocilja (2008)
Chip-based waveguides for high-sensitivity biosensing and super-resolution imaging
Chen-lei Pang (2020)
Optical biosensor for environmental on-line monitoring of naphthalene and salicylate bioavailability with an immobilized bioluminescent catabolic reporter bacterium.
A. Heitzer (1994)See more