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Antigen Retrieval In Immunohistochemistry

S.-R. Shi, C. Taylor
Published 2014 · Biology

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The antigen retrieval (AR) technique of boiling formalin-fixed, paraffin-embedded (FFPE) tissue sections in water is a highly effective method to render immunohistochemical (IHC) staining on FFPE tissue sections, which is a revolution in pathology and a milestone in IHC. The authors intend briefly to summarize developments in AR-IHC-based research and diagnostics, with particular emphasis on current challenges and future research directions based on our data and a wide literature review. This article includes basic principles and essential techniques of AR and several major issues in terms of standardization and quantitative IHC based on AR, extraction of nucleic acids and proteins from FFPE tissue for genomic and proteomic analysis, molecular mechanism of AR, and other related topics. Currently, the AR-IHC-based research is in developing, with describing our plan for future study, we wish that combining the proteomics approach with AR-IHC to establish a sophisticated platform for identifying biomarkers in personalized medicine.
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