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Microarray Techniques For More Rapid Protein Quantification: Use Of Single Spot Multiplex Analysis And A Vibration Reaction Unit.

Yukie Sasakura, Katsuhiro Kanda, S. Fukuzono
Published 2006 · Medicine, Chemistry

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Protein microarray technology is a powerful, popular tool for the high-throughput analysis of protein interactions. One important use for protein microarray technology is protein quantification by immunoassay, which was originally based on enzyme linked immunosorbent assay (ELISA) methods. Recently, new research and diagnostic applications have created a need for a rapid and easily applied high-throughput protein quantification method. Here, we introduce several novel techniques that address these needs. Our improved protein microarray-based sandwich immunoassay techniques allow researchers to: (1) control the size and shape of protein spots on the microarray using a perforated seal; (2) analyze two proteins within a single spot, thus increasing the number of tests run on a single microarray without increasing the number of protein spots; (3) improve the efficiency and speed of the Ag-Ab interaction through vibratory reagent convection, which increased the signal intensity by more than two-fold and decreased the reaction time from 30 to 10 min. These new techniques will facilitate rapid immunoassays for diagnostic purposes and other research areas utilizing protein microarray analysis, such as investigations of ligand-receptor or protein-small molecule interactions.
This paper references
Proteomic profiling of the NCI-60 cancer cell lines using new high-density reverse-phase lysate microarrays
S. Nishizuka (2003)
Fabrication of microfluidic systems in poly(dimethylsiloxane)
J. C. Mcdonald (2000)
Miniaturised multiplexed immunoassays.
T. Joos (2002)
Protein microarrays: Promising tools for proteomic research
M. Templin (2003)
Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
B. Haab (2000)
Protein microarrays: catching the proteome
O. Poetz (2005)
Profiling receptor tyrosine kinase activation by using Ab microarrays
U. Nielsen (2003)
Simultaneous multianalysis for tumor markers by antibody fragments microarray system
Shiping Song (2004)
Protein microarrays: Molecular profiling technologies for clinical specimens
V. Espina (2003)
Advances in recombinant antibody microarrays.
P. Pavlickova (2004)
ProteoChip: A highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein‐protein interaction studies
Yoonsuk Lee (2003)
Allergen microarray: comparison of microarray using recombinant allergens with conventional diagnostic methods to detect allergen‐specific serum immunoglobulin E
B. Jahn-Schmid (2003)
Protein microarray system for detecting protein-protein interactions using an anti-His-tag antibody and fluorescence scanning: effects of the heme redox state on protein-protein interactions of heme-regulated phosphodiesterase from Escherichia coli.
Yukie Sasakura (2004)
Microfluidic devices for fluidic circulation and mixing improve hybridization signal intensity on DNA arrays.
P. K. Yuen (2003)
Use of Reverse Phase Protein Microarrays and Reference Standard Development for Molecular Network Analysis of Metastatic Ovarian Carcinoma*
K. Sheehan (2005)
Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies
S. Sia (2003)
Analyzing antibody specificity with whole proteome microarrays
G. Michaud (2003)
Autoantigen microarrays for multiplex characterization of autoantibody responses
W. Robinson (2002)
Antigen microarrays for serodiagnosis of infectious diseases.
L. Mezzasoma (2002)
An easy setup for double slide microarray hybridization.
Abigail C Ting (2003)
Investigation of the relationship between protein-protein interaction and catalytic activity of a heme-regulated phosphodiesterase from Escherichia coli (Ec DOS) by protein microarray.
Yukie Sasakura (2005)
A Nonredundant Human Protein Chip for Antibody Screening and Serum Profiling*
A. Lueking (2003)
Printing proteins as microarrays for high-throughput function determination.
G. MacBeath (2000)
Protein microarray technology.
M. Templin (2002)

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