Online citations, reference lists, and bibliographies.
← Back to Search

Microarray Techniques For More Rapid Protein Quantification: Use Of Single Spot Multiplex Analysis And A Vibration Reaction Unit.

Yukie Sasakura, Katsuhiro Kanda, S. Fukuzono
Published 2006 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
Protein microarray technology is a powerful, popular tool for the high-throughput analysis of protein interactions. One important use for protein microarray technology is protein quantification by immunoassay, which was originally based on enzyme linked immunosorbent assay (ELISA) methods. Recently, new research and diagnostic applications have created a need for a rapid and easily applied high-throughput protein quantification method. Here, we introduce several novel techniques that address these needs. Our improved protein microarray-based sandwich immunoassay techniques allow researchers to: (1) control the size and shape of protein spots on the microarray using a perforated seal; (2) analyze two proteins within a single spot, thus increasing the number of tests run on a single microarray without increasing the number of protein spots; (3) improve the efficiency and speed of the Ag-Ab interaction through vibratory reagent convection, which increased the signal intensity by more than two-fold and decreased the reaction time from 30 to 10 min. These new techniques will facilitate rapid immunoassays for diagnostic purposes and other research areas utilizing protein microarray analysis, such as investigations of ligand-receptor or protein-small molecule interactions.
This paper references
10.1073/pnas.2331323100
Proteomic profiling of the NCI-60 cancer cell lines using new high-density reverse-phase lysate microarrays
S. Nishizuka (2003)
10.1002/(SICI)1522-2683(20000101)21:1<27::AID-ELPS27>3.0.CO;2-C
Fabrication of microfluidic systems in poly(dimethylsiloxane)
J. C. Mcdonald (2000)
10.1016/S1367-5931(01)00289-7
Miniaturised multiplexed immunoassays.
T. Joos (2002)
10.1002/PMIC.200300600
Protein microarrays: Promising tools for proteomic research
M. Templin (2003)
10.1186/gb-2001-2-2-research0004
Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions
B. Haab (2000)
10.1016/j.mad.2004.09.030
Protein microarrays: catching the proteome
O. Poetz (2005)
10.1073/pnas.1633513100
Profiling receptor tyrosine kinase activation by using Ab microarrays
U. Nielsen (2003)
10.1016/J.ACA.2004.01.020
Simultaneous multianalysis for tumor markers by antibody fragments microarray system
Shiping Song (2004)
10.1002/PMIC.200300592
Protein microarrays: Molecular profiling technologies for clinical specimens
V. Espina (2003)
10.1016/J.CCCN.2004.01.009
Advances in recombinant antibody microarrays.
P. Pavlickova (2004)
10.1002/PMIC.200300541
ProteoChip: A highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein‐protein interaction studies
Yoonsuk Lee (2003)
10.1046/j.1365-2222.2003.01784.x
Allergen microarray: comparison of microarray using recombinant allergens with conventional diagnostic methods to detect allergen‐specific serum immunoglobulin E
B. Jahn-Schmid (2003)
10.1021/AC048832T
Protein microarray system for detecting protein-protein interactions using an anti-His-tag antibody and fluorescence scanning: effects of the heme redox state on protein-protein interactions of heme-regulated phosphodiesterase from Escherichia coli.
Yukie Sasakura (2004)
10.1039/B210274A
Microfluidic devices for fluidic circulation and mixing improve hybridization signal intensity on DNA arrays.
P. K. Yuen (2003)
10.1074/mcp.T500003-MCP200
Use of Reverse Phase Protein Microarrays and Reference Standard Development for Molecular Network Analysis of Metastatic Ovarian Carcinoma*
K. Sheehan (2005)
10.1002/ELPS.200305584
Microfluidic devices fabricated in Poly(dimethylsiloxane) for biological studies
S. Sia (2003)
10.1038/nbt910
Analyzing antibody specificity with whole proteome microarrays
G. Michaud (2003)
10.1038/nm0302-295
Autoantigen microarrays for multiplex characterization of autoantibody responses
W. Robinson (2002)
10.1093/CLINCHEM/48.1.121
Antigen microarrays for serodiagnosis of infectious diseases.
L. Mezzasoma (2002)
10.2144/03354MT01
An easy setup for double slide microarray hybridization.
Abigail C Ting (2003)
10.1021/BI050406U
Investigation of the relationship between protein-protein interaction and catalytic activity of a heme-regulated phosphodiesterase from Escherichia coli (Ec DOS) by protein microarray.
Yukie Sasakura (2005)
10.1074/mcp.T300001-MCP200
A Nonredundant Human Protein Chip for Antibody Screening and Serum Profiling*
A. Lueking (2003)
10.1126/SCIENCE.289.5485.1760
Printing proteins as microarrays for high-throughput function determination.
G. MacBeath (2000)
10.1016/S1359-6446(00)01910-2
Protein microarray technology.
M. Templin (2002)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar