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Mitochondrial Metabolic States Regulate Nitric Oxide And Hydrogen Peroxide Diffusion To The Cytosol.
A. Boveris, L. Valdez, T. Zaobornyj, J. Bustamante
Published 2006 · Chemistry, Medicine
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Mitochondria isolated from rat heart, liver, kidney and brain (respiratory control 4.0-6.5) release NO and H2O2 at rates that depend on the mitochondrial metabolic state: releases are higher in state 4, about 1.7-2.0 times for NO and 4-16 times for H2O2, than in state 3. NO release in rat liver mitochondria showed an exponential dependence on membrane potential in the range 55 to 180 mV, as determined by Rh-123 fluorescence. A similar behavior was reported for mitochondrial H2O2 production by [S.S. Korshunov, V.P. Skulachev, A.A. Starkov, High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria. FEBS Lett. 416 (1997) 15_18.]. Transition from state 4 to state 3 of brain cortex mitochondria was associated to a decrease in NO release (50%) and in membrane potential (24-53%), this latter determined by flow cytometry and DiOC6 and JC-1 fluorescence. The fraction of cytosolic NO provided by diffusion from mitochondria was 61% in heart, 47% in liver, 30% in kidney, and 18% in brain. The data supports the speculation that NO and H2O2 report a high mitochondrial energy charge to the cytosol. Regulation of mtNOS activity by membrane potential makes mtNOS a regulable enzyme that in turn regulates mitochondrial O2 uptake and H2O2 production.
This paper references
The respiratory chain and oxidative phosphorylation.
B. Chance (1956)
Inhibition of electron and energy transfer in mitochondria by 19-nor-ethynyltestosterone acetate.
A. Boveris (1970)
The cellular production of hydrogen peroxide.
A. Boveris (1972)
The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen.
A. Boveris (1973)
Regulation of uptake of purines, pyrimidines and amino acids by Candida utilis.
R. W. Jones (1973)
Enhancement of hydrogen peroxide formation by protophores and ionophores in antimycin-supplemented mitochondria.
E. Cadenas (1980)
Determination of the production of superoxide radicals and hydrogen peroxide in mitochondria.
A. Boveris (1984)
Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties.
R. Emaus (1986)
Nanomolar concentrations of nitric oxide reversibly inhibit synaptosomal respiration by competing with oxygen at cytochrome oxidase
G. Brown (1994)
 Nitric oxide assay using hemoglobin method
M. E. Murphy (1994)
Reversible inhibition of cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, by nitric oxide
M. Cleeter (1994)
Nitric oxide inhibits electron transfer and increases superoxide radical production in rat heart mitochondria and submitochondrial particles.
J. Poderoso (1996)
Nitric oxide diffusion in membranes determined by fluorescence quenching.
A. Denicola (1996)
Dependence of H2O2 Formation by Rat Heart Mitochondria on Substrate Availability and Donor Age
R. Hansford (1997)
Formation and properties of peroxynitrite as studied by laser flash photolysis, high-pressure stopped-flow technique, and pulse radiolysis.
R. Kissner (1997)
Oxygen, Gene Expression, and Cellular Function
D. Massaro (1997)
High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria
S. S. Korshunov (1997)
The Regulation of Mitochondrial Oxygen Uptake by Redox Reactions Involving Nitric Oxide and Ubiquinol*
J. Poderoso (1999)
Understanding the Process of Aging : The Roles of Mitochondria: Free Radicals, and Antioxidants
L. Packer (1999)
Regulation of mitochondrial respiration by adenosine diphosphate, oxygen, and nitric oxide.
A. Boveris (1999)
Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives.
R. C. Scaduto (1999)
Pharmacological regulation of mitochondrial nitric oxide synthase.
A. Boveris (2002)
Sequential NO production by mitochondria and endoplasmic reticulum during induced apoptosis.
J. Bustamante (2002)
Biochemistry of Mitochondrial Nitric-oxide Synthase*
S. Elfering (2002)
c-Jun N-terminal kinase (JNK)-mediated modulation of brain mitochondria function: new target proteins for JNK signalling in mitochondrion-dependent apoptosis.
H. Schroeter (2003)
Mitochondrial Biogenesis in Mammals: The Role of Endogenous Nitric Oxide
E. Nisoli (2003)
Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F(0) during ATP synthesis.
A. Baracca (2003)
Kidney mitochondrial nitric oxide synthase.
A. Boveris (2003)
Regulation of brain mitochondrial H2O2 production by membrane potential and NAD(P)H redox state
A. Starkov (2003)
Mitochondrial nitric oxide synthase drives redox signals for proliferation and quiescence in rat liver development
M. Carreras (2004)
Mitochondrial nitric oxide metabolism in rat muscle during endotoxemia.
S. Álvarez (2004)
On the mechanism and biology of cytochrome oxidase inhibition by nitric oxide.
F. Antunes (2004)
The powerhouse takes control of the cell; the role of mitochondria in signal transduction.
V. Darley‐Usmar (2004)
Disruption of mitochondrial membrane potential during apoptosis induced by PSC 833 and CsA in multidrug-resistant lymphoid leukemia.
J. Bustamante (2004)
Brain mitochondrial nitric oxide synthase: in vitro and in vivo inhibition by chlorpromazine.
S. Lores-Arnaiz (2004)
Mitochondrial function and mitochondria-induced apoptosis in an overstimulated rat ovarian cycle.
A. Navarro (2005)
Functional activity of mitochondrial nitric oxide synthase.
L. Valdez (2005)
Heart mitochondrial nitric oxide synthase is upregulated in male rats exposed to high altitude (4,340 m).
G. Gonzales (2005)
Nitroxia: the pathological consequence of dysfunction in the nitric oxide-cytochrome c oxidase signaling pathway.
S. Shiva (2005)
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Implication of Mitochondrial NO/cGMP/PKG Signaling System in the Activation and Inhibition of Mitochondrial Respiration by L-Arginine and NO Donors
V. V. Dynnik (2019)
(+)-Catechin inhibits heart mitochondrial complex I and nitric oxide synthase: functional consequences on membrane potential and hydrogen peroxide production.
D. Iglesias (2019)
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Belén Mendoza-Chamizo (2018)
Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration
L. Valdez (2018)
Potential protective role of nitric oxide and Hsp70 linked to functional foods in the atherosclerosis
A. Camargo (2017)
Potencial rol protector del óxido nítrico y Hsp70 asociado a alimentos funcionales en la aterosclerosis
A. B. Camargo (2017)
Hydrogen peroxide, nitric oxide and ATP are molecules involved in cardiac mitochondrial biogenesis in Diabetes.
Silvina S. Bombicino (2017)
Hypoxia in Obesity and Diabetes: Potential Therapeutic Effects of Hyperoxia and Nitrate
Reza Norouzirad (2017)
Not breathing is not an option: How to deal with oxidative DNA damage.
Enni Markkanen (2017)
Effects of sodium pyruvate on viability, synthesis of reactive oxygen species, lipid peroxidation and DNA integrity of cryopreserved bovine sperm.
Ferhan Korkmaz (2017)
Ischemic postconditioning protects the heart against ischemia–reperfusion injury via neuronal nitric oxide synthase in the sarcoplasmic reticulum and mitochondria
L. Hu (2016)
Thioredoxin-1 Attenuates Ventricular and Mitochondrial Postischemic Dysfunction in the Stunned Myocardium of Transgenic Mice.
Virginia Pérez (2016)
Biochemistry and Physiology of Heart Mitochondrial Nitric Oxide Synthase
T. Zaobornyj (2016)
Mitochondrial nitric oxide production supported by reverse electron transfer.
S. Bombicino (2016)
Mitochondrial Complex I Inactivation After Ischemia-Reperfusion in the Stunned Heart
L. Valdez (2016)
Diabetes impairs heart mitochondrial function without changes in resting cardiac performance.
Silvina S. Bombicino (2016)
Integrated High-Content Quantification of Intracellular ROS Levels and Mitochondrial Morphofunction.
Tom Sieprath (2016)
Alterations of cellular redox homeostasis in cultured fibroblast-like renal cells upon exposure to low doses of cytochrome bc1 complex inhibitor kresoxim-methyl.
E. Flampouri (2016)
Thioredoxin Attenuates Post-ischemic Damage in Ventricular and Mitochondrial Function
V. D’annunzio (2016)
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Haider H. Humaish (2016)
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Adrián Aicardo (2016)
Epigenetic Changes in Endothelial Progenitors as a Possible Cellular Basis for Glycemic Memory in Diabetic Vascular Complications
P. Rajasekar (2015)
Interactions between mitochondrial reactive oxygen species and cellular glucose metabolism
D. C. Liemburg-Apers (2015)
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