Online citations, reference lists, and bibliographies.
← Back to Search

In The Early Phase Of Programmed Cell Death In Tobacco Bright Yellow 2 Cells The Mitochondrial Adenine Nucleotide Translocator, Adenylate Kinase And Nucleoside Diphosphate Kinase Are Impaired In A Reactive Oxygen Species-dependent Manner.

D. Valenti, R. A. Vacca, M. D. de Pinto, L. De Gara, E. Marra, S. Passarella
Published 2007 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
To investigate whether and how mitochondria can change in plant programmed cell death (PCD), we used the non-photosynthetic Tobacco Bright Yellow 2 (TBY-2) cells. These can be synchronized to high levels, stand out in terms of growth rate and homogeneity and undergo PCD as a result of heat shock. Using these cells we investigated the activity of certain mitochondrial proteins that have a role in providing ATP and/or other nucleoside triphosphates (NTPs). We show that, already after 2 h from the heat shock, when cell viability remains unaffected, the rate of ADP/ATP exchange due to adenine nucleotide translocator (ANT) activity, and the rate of the reactions catalysed by adenylate kinase (ADK; EC and nucleoside diphosphate kinase (NDPK; EC are inhibited in a non-competitive-like manner. In all cases, externally added ascorbate partially prevented the inhibition. These effects occurred in spite of minor (for ANT) or no changes in the mitochondrial protein levels as immunologically investigated. Interestingly, a decrease of both the steady state level of the ascorbate pool and of the activity of l-galactono-gamma-lactone dehydrogenase (GLDH) (EC, the mitochondrial enzyme catalysing the last step of ascorbate biosynthesis, were also found.
This paper references
Catalytic metals, ascorbate and free radicals: combinations to avoid.
G. Buettner (1996)
P 1 ,P 5 -Di(adenosine-5')pentaphosphate, a potent multisubstrate inhibitor of adenylate kinase.
G. Lienhard (1973)
Oxidative Stress Increased Respiration and Generation of Reactive Oxygen Species, Resulting in ATP Depletion, Opening of Mitochondrial Permeability Transition, and Programmed Cell Death1
B. Tiwari (2002)
Cooperation and competition between adenylate kinase , nucleoside diphosphokinase , electron transport , and ATP synthase in plant mitochondria studied by 31 P - nuclear magnetic resonance
S. Aubert (2000)
An increase in the ATP levels occurs in cerebellar granule cells en route to apoptosis in which ATP derives from both oxidative phosphorylation and anaerobic glycolysis.
A. Atlante (2005)
Control of Ascorbate Synthesis by Respiration and Its Implications for Stress Responses1
A. Millar (2003)
Enzymes of the ascorbate biosynthesis and ascorbate-glutathione cycle in cultured cells of tobacco Bright Yellow 2
M. C. Pinto (2000)
Ascorbate biosynthesis: a diversity of pathways
N. Smirnoff (2004)
Vitamin C : Its Functions and Biochemistry in Animals and Plants
H. Asard (2004)
Mitochondrial apoptotic pathways.
N. Mohamad (2005)
Does the plant mitochondrion integrate cellular stress and regulate programmed cell death?
A. Jones (2000)
Programmed cell death.
R. Lockshin (1974)
Biosynthesis of ascorbic acid in kidney bean. L-galactono-gamma-lactone dehydrogenase is an intrinsic protein located at the mitochondrial inner membrane
Siendones (1999)
Production of Reactive Oxygen Species, Alteration of Cytosolic Ascorbate Peroxidase, and Impairment of Mitochondrial Metabolism Are Early Events in Heat Shock-Induced Programmed Cell Death in Tobacco Bright-Yellow 2 Cells1
R. A. Vacca (2004)
Programmed cell death via mitochondria: Different modes of dying
M. Bras (2005)
Secemski, P1,P5-Di(adenosine-5′)pentaphosphate, a potent multisubstrate inhibitor of adenylate kinase
I.I.G.E. Lienhard (1973)
AZT inhibition of the ADP/ATP antiport in isolated rat heart mitochondria.
D. Valenti (2000)
Factors determining the relative contribution of the adenine-nucleotide translocator and the ADP-regenerating system to the control of oxidative phosphorylation in isolated rat-liver mitochondria.
R. Wanders (1984)
Caspase‐dependent alteration of the ADP/ATP translocator triggers the mitochondrial permeability transition which is not required for the low‐potassium‐dependent apoptosis of cerebellar granule cells
A. Atlante (2006)
Glucose-6-phosphate Dehydrogenase
G. Löhr (1974)
The role of mitochondrial transport in energy metabolism.
S. Passarella (2003)
Initiation of Apoptotic Signal by the Peroxidation of Cardiolipin of Mitochondria
Y. Nakagawa (2004)
A simple ultraviolet spectrophotometric method for the determination of protein.
W. Waddell (1956)
Reactive oxygen species and the mitochondrial signaling pathway of cell death.
M. Le Bras (2005)
Adenosine diphosphate translocation in mitochondria. Nature of the receptor site for carboxyatractyloside (gummiferin).
P. Vignais (1973)
The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco BY-2 cells
M. C. Pinto (2005)
The redox state of the ascorbatedehydroascorbate pair as a specific sensor of cell division in tobacco
M. C. de Pinto (1999)
Programmed cell death, mitochondria and the plant hypersensitive response
Eric Lam (2001)
Changes in the Antioxidant Systems as Part of the Signaling Pathway Responsible for the Programmed Cell Death Activated by Nitric Oxide and Reactive Oxygen Species in Tobacco Bright-Yellow 2 Cells1
M. D. de Pinto (2002)
Cytochrome c Is Released in a Reactive Oxygen Species-Dependent Manner and Is Degraded via Caspase-Like Proteases in Tobacco Bright-Yellow 2 Cells en Route to Heat Shock-Induced Cell Death1
R. A. Vacca (2006)
A. Needham (1965)
Methods of Enzymatic Analysis
H. U. Bergmeyer (1963)
The biosynthetic pathway of vitamin C in higher plants
G. Wheeler (1998)
Mitochondrial role in life and death of the cell.
H. Lee (2000)
Cooperation and Competition between Adenylate Kinase, Nucleoside Diphosphokinase, Electron Transport, and ATP Synthase in Plant Mitochondria Studied by 31P-Nuclear Magnetic Resonance
J. Roberts (1997)
Ascorbate biosynthesis in mitochondria is linked to the electron transport chain between complexes III and IV.
C. Bartoli (2000)
Plant mitochondrial nucleoside diphosphate kinase is attached to the membrane through interaction with the adenine nucleotide translocator
C. Knorpp (2003)
Many ways to exit? Cell death categories in plants.
W. G. van Doorn (2005)
Hydrogen peroxide, nitric oxide and cytosolic ascorbate peroxidase at the crossroad between defence and cell death.
M. D. de Pinto (2006)
Enzymatic assay of fumarase
H. U. Bergmeyer (1974)
Il Liceo (1824)
Does the plant mitochondrion integrate cellular stress and regulate programmed cell death
J. E. Taylor (2000)
Cytochrome c, released from cerebellar granule cells undergoing apoptosis or excytotoxic death, can generate protonmotive force and drive ATP synthesis in isolated mitochondria
A. Atlante (2003)

This paper is referenced by
Investigating the role of respiration in plant salinity tolerance by analyzing mitochondrial proteomes from wheat and a salinity-tolerant Amphiploid (wheat × Lophopyrum elongatum).
R. P. Jacoby (2013)
Cleavage of DNA and nuclease properties of Plasmodium Nucleoside Diphosphate Kinase
Abdullah Altaher (2014)
1 Supporting Information : Alternative paths of some of the maximally-favourable reactions
Chloroplast Retrograde Regulation of Heat Stress Responses in Plants
Ai-Zhen Sun (2016)
Proteasome function is required for activation of programmed cell death in heat shocked tobacco Bright‐Yellow 2 cells
R. A. Vacca (2007)
Impairment of F1F0-ATPase, adenine nucleotide translocator and adenylate kinase causes mitochondrial energy deficit in human skin fibroblasts with chromosome 21 trisomy.
D. Valenti (2010)
Characterization and redesign of galactonolactone dehydrogenase, a flavoprotein producing vitamin C
N. Leferink (2009)
Clues to the functions of plant NDPK isoforms
S. Dorion (2014)
Role of redox homeostasis in thermo-tolerance under a climate change scenario.
M. D. de Pinto (2015)
Simple, reliable, and time-efficient colorimetric method for the assessment of mitochondrial function and toxicity.
Faraz S. Ahmad (2018)
Different involvement of the mitochondrial, plastidial and cytosolic ascorbate-glutathione redox enzymes in heat shock responses.
Vittoria Locato (2009)
Mitochondrial proteome during salt stress-induced programmed cell death in rice.
X. Chen (2009)
Novel functions of the mitochondrial nucleoside diphosphate kinase in plants
Jenni Hammargren (2007)
Vitamin C in Plants: From Functions to Biofortification
C. Paciolla (2019)
Wheat mitochondrial proteomes provide new links between antioxidant defense and plant salinity tolerance.
R. P. Jacoby (2010)
The activities of nucleoside diphosphate kinase and adenylate kinase are influenced by their interaction
M. Johansson (2008)
Nitric oxide suppresses aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips by improving mitochondrial physiological properties.
H. He (2018)
Galactonolactone Dehydrogenase Requires a Redox-Sensitive Thiol for Optimal Production of Vitamin C1
Nicole G H Leferink (2009)
Dysfunction of cortical synapse-specific mitochondria in developing rats exposed to lead and its amelioration by ascorbate supplementation
F. Ahmad (2018)
Redox Balance-DDR-miRNA Triangle: Relevance in Genome Stability and Stress Responses in Plants
Sara Cimini (2019)
Deficit of complex I activity in human skin fibroblasts with chromosome 21 trisomy and overproduction of reactive oxygen species by mitochondria: involvement of the cAMP/PKA signalling pathway.
D. Valenti (2011)
Induction of Programmed cell death in tobacco Bright Yellow-2 cell lines and Saccharomyces cerevisiae strainYPH-500 against various apoptotic stimuli
D. L. Reddy (2008)
The pea mitochondrial nucleoside diphosphate kinase cleaves DNA and RNA
Jenni Hammargren (2007)
Expression of Animal Anti-Apoptotic Gene Ced-9 Enhances Tolerance during Glycine max L.–Bradyrhizobium japonicum Interaction under Saline Stress but Reduces Nodule Formation
G. Robert (2014)
Vitamin C: The Metabolism and Functions of Ascorbic Acid in Plants
N. Smirnoff (2011)
Nitric Oxide and Reactive Oxygen Species in PCD Signaling
V. Locato (2016)
Exploring the soluble proteome of Tobacco Bright Yellow-2 cells at the switch towards different cell fates in response to heat shocks.
Milena Marsoni (2010)
Redox regulation in plant programmed cell death.
M. D. de Pinto (2012)
The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status
T. A. Giancaspero (2009)
Semantic Scholar Logo Some data provided by SemanticScholar