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Characterization Of Pancreatic Lipase-related Protein 2 Isolated From Human Pancreatic Juice.
J. De Caro, B. Sias, P. Grandval, F. Ferrato, H. Halimi, F. Carrière, A. De caro
Published 2004 · Biology, Medicine
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Human pancreatic lipase-related protein 2 (HPLRP2) was identified for the first time in pancreatic juice using specific anti-peptide antibodies and purified to homogeneity. Antibodies were raised in the rabbit using a synthetic peptide from the HPLRP2 protein sequence deduced from cDNA. Western blotting analysis showed that these antibodies did not react with classical human pancreatic lipase (HPL) or human pancreatic lipase-related protein 1 (HPLRP1) but cross-reacted with native rat PLRP2 (RPLRP2), as well as with recombinant rat and guinea-pig PLRP2 (GPLRP2). Immunoaffinity chromatography was performed on immobilized anti-recombinant HPLRP2 polyclonal antibodies to purify native HPLRP2 after conventional chromatographic steps including gel filtration and chromatrography on an anion-exchanger. The substrate specificity of HPLRP2 was investigated using various triglycerides, phospholipids and galactolipids as substrates. The lipase activity on triglycerides was inhibited by bile salts and weakly restored by colipase. The phospholipase activity of HPLRP2 on phospholipid micelles was very low. A significant level of galactolipase activity was measured using monogalactosyldiglyceride monomolecular films. These data suggest that the main physiological function of HPLRP2 is the hydrolysis of galactolipids, which are the main lipids present in vegetable food.
This paper references
Decreased Neonatal Dietary Fat Absorption and T Cell Cytotoxicity in Pancreatic Lipase-related Protein 2-Deficient Mice*
M. Lowe (1998)
Two novel human pancreatic lipase related proteins, hPLRP1 and hPLRP2. Differences in colipase dependence and in lipase activity.
T. Giller (1992)
Interfacial activation of the lipase–procolipase complex by mixed micelles revealed by X-ray crystallography
H. V. Tilbeurgh (1993)
Structure of human pancreatic lipase
F. Winkler (1990)
Structure of the canine pancreatic lipase gene.
F. S. Mickel (1989)
Structure and Activity of Rat Pancreatic Lipase-related Protein 2*
A. Roussel (1998)
Enzyme reactions in a membrane model. 1. A new technique to study enzyme reactions in monolayers.
R. Verger (1973)
Stabilization of the C-terminal part of pig and horse colipase by carboxypeptidase and trypsin inhibitors.
C. Chapus (1981)
Pancreatic lipase-related protein 1 (PLRP1) is present in the pancreatic juice of several species.
J. De Caro (1998)
Structure-function relationships in naturally occurring mutants of pancreatic lipase.
F. Carrière (1994)
Rat pancreatic lipase and two related proteins: enzymatic properties and mRNA expression during development.
R. Payne (1994)
A structural domain (the lid) found in pancreatic lipases is absent in the guinea pig (phospho)lipase.
A. Hjorth (1993)
Identification and cloning of GP-3 from rat pancreatic acinar zymogen granules as a glycosylated membrane-associated lipase.
M. J. Wishart (1993)
Pancreatic lipase structure-function relationships by domain exchange.
F. Carrière (1997)
Substrate specificity of two cationic lipases with high phospholipase A1 activity purified from guinea pig pancreas. II. Studies on glycerophospholipids.
J. Fauvel (1984)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
Cloning and expression in insect cells of two pancreatic lipases and a procolipase from Myocastor coypus.
K. Thirstrup (1995)
Purification and properties of phospholipase A from porcine pancreas.
G. D. de Haas (1968)
Discoordinate Expression of Pancreatic Lipase and Two Related Proteins in the Human Fetal Pancreas
Yanqing Yang (2000)
The human pancreatic lipase-encoding gene: structure and conservation of an Alu sequence in the lipase gene family.
H. Sims (1993)
Physiology of Gastrointestinal Lipolysis and Therapeutical Use of Lipases and Digestive Lipase Inhibitors
H. Lengsfeld (2005)
Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter.
J. Gershoni (1982)
Purification of two lipases with high phospholipase A1 activity from guinea-pig pancreas.
J. Fauvel (1981)
Rat GP-3 is a pancreatic lipase with kinetic properties that differ from colipase-dependent pancreatic lipase.
M. Jennens (1995)
Critical Evaluation of a Specific ELISA and Two Enzymatic Assays of Pancreatic Lipases in Human Sera
P. Grandval (2004)
Genomic organization of the murine CTL lipase gene.
M. Kaplan (1996)
Reactivation of the totally inactive pancreatic lipase RP1 by structure‐predicted point mutations
A. Roussel (1998)
A pancreatic lipase with a phospholipase A1 activity: crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig.
C. Withers-Martinez (1996)
Hydrolysis of galactolipids by human pancreatic lipolytic enzymes and duodenal contents.
L. Andersson (1995)
GP-3, a newly characterized glycoprotein on the inner surface of the zymogen granule membrane, undergoes regulated secretion.
A. Wagner (1994)
Human pancreatic lipase: a glycoprotein.
A. De caro (1977)
High expression in adult horse of PLRP2 displaying a low phospholipase activity.
S. Jayne (2002)
Evidence for a pancreatic lipase subfamily with new kinetic properties.
K. Thirstrup (1994)
Pancreatic lipase-related protein type I: a specialized lipase or an inactive enzyme.
I. Crenon (1998)
Isolation and properties of prophospholipase A2 from human pancreatic juice.
R. Grataroli (1981)
Purification and properties of five different forms of human procarboxypeptidases.
R. Pascual (1989)
Cloning of an interleukin-4 inducible gene from cytotoxic T lymphocytes and its identification as a lipase
M. Grusby (1990)
Pancreatic lipase-related protein 2 but not classical pancreatic lipase hydrolyzes galactolipids.
L. Andersson (1996)
One-step purification and biochemical characterization of recombinant pancreatic lipases expressed in insect cells.
S. Bezzine (1999)
Evidence for the lack of classical secretory phospholipase A2 in guinea-pig pancreas.
J. Fauvel (1981)
This paper is referenced by
Pancreatic lipase‐related protein 2 is essential for egg hatching in the brown planthopper, Nilaparvata lugens
L. Xu (2017)
Continuous measurement of galactolipid hydrolysis by pancreatic lipolytic enzymes using the pH-stat technique and a medium chain monogalactosyl diglyceride as substrate.
S. Amara (2009)
Lipid‐dependent cytotoxicity by the lipase PLRP2 and by PLRP2‐positive cytotoxic T lymphocytes (CTLs)
Bryce N. Alves (2009)
Synthesis and kinetic evaluation of cyclophostin and cyclipostins phosphonate analogs as selective and potent inhibitors of microbial lipases.
V. Point (2012)
Pancreatic triglyceride lipase mediates lipotoxic systemic inflammation.
C. D. de Oliveira (2020)
Pancreatic and mucosal enzymes in choline phospholipid digestion.
Å. Nilsson (2019)
Pancreatic lipase and pancreatic lipase-related protein 2, but not pancreatic lipase-related protein 1, hydrolyze retinyl palmitate in physiological conditions.
E. Reboul (2006)
Analysis of the discriminative inhibition of mammalian digestive lipases by 3-phenyl substituted 1,3,4-oxadiazol-2(3H)-ones.
V. Point (2012)
In vitro digestion of galactolipids from chloroplast-rich fraction (CRF) of postharvest, pea vine field residue (haulm) and spinach leaves.
Jutarat Wattanakul (2019)
Role of the Lid Hydrophobicity Pattern in Pancreatic Lipase Activity*
Annick Thomas (2005)
Occurrence of pancreatic lipase-related protein-2 in various species and its relationship with herbivore diet.
J. De Caro (2008)
Inhibition of phospholipase A1, lipase and galactolipase activities of pancreatic lipase-related protein 2 by methyl arachidonyl fluorophosphonate (MAFP).
S. Amara (2012)
Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells Published, JLR Papers in Press, March 30, 2007.
C. Eydoux (2007)
Lipolysis of the semi-solid self-emulsifying excipient Gelucire 44/14 by digestive lipases.
S. Fernandez (2008)
In vitro lipolysis tests on lipid nanoparticles: comparison between lipase/co-lipase and pancreatic extract
V. Jannin (2015)
Dissecting the Interaction Deficiency of a Cartilaginous Fish Digestive Lipase with Pancreatic Colipase: Biochemical and Structural Insights
N. Achouri (2020)
Comparative study on digestive lipase activities on the self emulsifying excipient Labrasol, medium chain glycerides and PEG esters.
S. Fernandez (2007)
BSSL and PLRP 2 : key enzymes for lipid digestion in the newborn examined using the Caco-2 cell line
Eva-Lotta Andersson ()
Role of the structural domains in the functional properties of pancreatic lipase‐related protein 2
Amélie Berton (2007)
The galactolipase activity of some microbial lipases and pancreatic enzymes
S. Amara (2013)
Gastrointestinal lipolysis of lipid-based excipients intended for the oral drug delivery of poorly water-soluble drugs
Sylvie Fernandez (2010)
IR spectroscopy analysis of pancreatic lipase-related protein 2 interaction with phospholipids: 1. Discriminative recognition of mixed micelles versus liposomes.
E. Mateos-Diaz (2018)
Postprandial effects on plasma lipids and satiety hormones from intake of liposomes made from fractionated oat oil: two randomized crossover studies
L. Ohlsson (2014)
Soixante ans de recherche sur la lipolyse enzymatique des corps gras à Marseille
F. Carrière (2008)
Evaluation of the recombinant turkey pancreatic lipase phospholipase activity: A monolayer study.
Madiha Bou Ali (2015)
Carboxyl Ester Lipase May Not Mediate Lipotoxic Injury during Severe Acute Pancreatitis.
Biswajit Khatua (2019)
Lipases and lipolysis in the human digestive tract: where do we stand?
M. Armand (2007)
Human pancreatic lipase-related protein 2: tissular localization along the digestive tract and quantification in pancreatic juice using a specific ELISA.
C. Eydoux (2006)
Slowing down fat digestion and absorption by an oxadiazolone inhibitor targeting selectively gastric lipolysis.
V. Point (2016)
Effects of non-ionic surfactants on in vitro triglyceride digestion and their susceptibility to digestion by pancreatic enzymes.
Anne Christiansen (2010)
Supported inhibitor for fishing lipases in complex biological media and mass spectrometry identification.
Vincent Delorme (2014)
Bis (monoacylglycero) phosphate interfacial properties and lipolysis by pancreatic lipase-related protein 2, an enzyme present in THP-1 human monocytes.
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