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Reorganization Of Actin Filaments Enhances Chondrogenic Differentiation Of Cells Derived From Murine Embryonic Stem Cells.

Z. Zhang, Joseph M Messana, N. Hwang, J. Elisseeff
Published 2006 · Biology, Medicine

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Differentiation of embryonic stem cells is of great interest to developmental biology and regenerative medicine. This study investigated the effects of cytochalasin D (CD) on the distribution of actin filaments in mouse embryoid body (EB)-derived cells. Furthermore, CD was applied to chondrogenic medium to examine its chondrogenic effect. CD at a concentration of 1 microg/ml disrupted stress fibers in EB-derived cells. Actin filaments in treated cells reorganized into a peripheral pattern, and type II collagen was detected by immunocytochemistry. The expression of type II collagen, Sox9, and at a later time point, aggrecan was up-regulated after CD treatment. In the CD-treated cells, Oct4 and Sox2, representing undifferentiation, were down-regulated as well as Sox1, AFP, and CTN-1, representing ectoderm, endoderm, and cardiogenesis, respectively. In conclusion, CD treatment enhances chondrogenesis of EB-derived cells. Moreover, it promotes a more complete stem cell differentiation toward chondrogenesis, when cultured in chondrogenic medium.
This paper references
10.1095/biolreprod.104.028100
Self-renewal vs. Differentiation of Mouse Embryonic Stem Cells1
K. O'Shea (2004)
10.1016/S1534-5807(04)00075-9
Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment.
R. McBeath (2004)
10.1074/jbc.M509433200
RhoA/ROCK Signaling Regulates Chondrogenesis in a Context-dependent Manner*
A. Woods (2006)
10.1083/JCB.106.1.171
Alterations in chondrocyte cytoskeletal architecture during phenotypic modulation by retinoic acid and dihydrocytochalasin B-induced reexpression
P. D. Brown (1988)
10.1634/stemcells.2004-0110
Musculoskeletal Differentiation of Cells Derived from Human Embryonic Germ Cells
M. Kim (2005)
10.1186/1471-213X-5-22
Comparison of the gene expression profile of undifferentiated human embryonic stem cell lines and differentiating embryoid bodies
Bhaskar Bhattacharya (2005)
10.1006/BBRC.2000.2987
Disruption of actin cytoskeleton induces chondrogenesis of mesenchymal cells by activating protein kinase C-alpha signaling.
Y. Lim (2000)
10.1053/JOCA.2001.0482
Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture.
M. Schnabel (2002)
10.1634/stemcells.2005-0024
Effects of Three‐Dimensional Culture and Growth Factors on the Chondrogenic Differentiation of Murine Embryonic Stem Cells
N. Hwang (2006)
10.1016/S0959-437X(97)80010-X
Early steps in vertebrate cardiogenesis.
T. Mohun (1997)
10.1002/jcb.10389
Chondrogenesis induced by actin cytoskeleton disruption is regulated via protein kinase C‐dependent p38 mitogen‐activated protein kinase signaling
Y. Lim (2003)
10.1074/JBC.M409158200
RhoA/ROCK Signaling Regulates Sox9 Expression and Actin Organization during Chondrogenesis*
A. Woods (2005)
10.1016/S1095-6433(01)00429-9
The cytoskeleton and cell volume regulation.
S. Pedersen (2001)
10.1634/stemcells.2005-0143
Assessing Self‐Renewal and Differentiation in Human Embryonic Stem Cell Lines
Jingli Cai (2006)
10.1006/EXCR.1993.1033
Dihydrocytochalasin B enhances transforming growth factor-beta-induced reexpression of the differentiated chondrocyte phenotype without stimulation of collagen synthesis.
P. Benya (1993)
10.1126/SCIENCE.1064829
Taking Cell-Matrix Adhesions to the Third Dimension
E. Cukierman (2001)
10.1186/1477-7827-2-41
Derivation and characterization of monkey embryonic stem cells
K. F. Pau (2004)
10.1083/JCB.105.4.1473
Effects of cytochalasin and phalloidin on actin
J. A. Cooper (1987)
10.1089/TEN.2006.12.2695
Chondrogenic differentiation of human embryonic stem cell-derived cells in arginine-glycine-aspartate-modified hydrogels.
N. Hwang (2006)
10.1002/JEMT.1070280503
Cytoskeleton of cartilage cells
M. Benjamin (1994)
10.1016/J.YDBIO.2004.02.005
Sox1 acts through multiple independent pathways to promote neurogenesis.
L. Kan (2004)
10.1634/stemcells.2004-0062
Directing Stem Cell Differentiation into the Chondrogenic Lineage In Vitro
B. Heng (2004)
10.1111/j.1469-445X.2000.02104.x
Embryoid Bodies: An In Vitro Model of Mouse Embryogenesis
I. Desbaillets (2000)
10.1083/JCB.99.1.115
Induction of chondrogenesis in limb mesenchymal cultures by disruption of the actin cytoskeleton
N. Zanetti (1984)
10.1177/002215540004801002
The Chondrocyte Cytoskeleton in Mature Articular Cartilage: Structure and Distribution of Actin, Tubulin, and Vimentin Filaments
E. Langelier (2000)
Phenotypic modulation of nasal septal chondrocytes by cytoskeleton modification.
S. Loty (2000)
10.1038/ncb0703-599
Conserved microtubule–actin interactions in cell movement and morphogenesis
O. C. Rodriguez (2003)
10.1002/jcb.20171
Chondrogenic differentiation of murine embryonic stem cells: Effects of culture conditions and dexamethasone
H. Tanaka (2004)



This paper is referenced by
10.1002/bdrc.20173
The role of mechanical signals in regulating chondrogenesis and osteogenesis of mesenchymal stem cells.
D. Kelly (2010)
10.1007/s00441-008-0705-6
Use of staurosporine, an actin-modifying agent, to enhance fibrochondrocyte matrix gene expression and synthesis
G. Hoben (2008)
10.1002/jcp.21110
Regulation of chondrocyte differentiation by the actin cytoskeleton and adhesive interactions
A. Woods (2007)
10.1007/s12015-010-9222-6
Potential of Human Embryonic Stem Cells in Cartilage Tissue Engineering and Regenerative Medicine
W. Toh (2010)
10.1002/jcb.22191
Transforming growth factor‐β3‐induced Smad signaling regulates actin reorganization during chondrogenesis of chick leg bud mesenchymal cells
D. Kim (2009)
10.1039/C1JM14401D
Biomimetic composites and stem cells interaction for bone and cartilage tissue regeneration
N. Naveena (2012)
10.1016/j.actbio.2012.01.034
Assessing embryonic stem cell response to surface chemistry using plasma polymer gradients.
F. Harding (2012)
The role of vitamin A during bio-mineral tissue development in pigs
A. Clark (2019)
10.1007/978-3-540-77755-7_45
Microenvironmental Determinants of Stem Cell Fate
R. Mauck (2009)
10.1016/j.actbio.2010.08.007
Mechano-topographic modulation of stem cell nuclear shape on nanofibrous scaffolds.
A. Nathan (2011)
10.1016/j.cellbi.2008.07.013
Integrity of the cortical actin ring is required for activation of the PI3K/Akt and p38 MAPK signaling pathways in redifferentiation of chondrocytes on chitosan
E. Park (2008)
10.1371/journal.pone.0067896
Silencing BRE Expression in Human Umbilical Cord Perivascular (HUCPV) Progenitor Cells Accelerates Osteogenic and Chondrogenic Differentiation
E. Chen (2013)
Development of hyaluronic acid – poly(ethylene glycol) hydrogels towards hematopoietic differentiation of mouse embryonic stem cells
Kathryn Marie Erickson (2009)
10.1093/hmg/ddu007
Filamin-interacting proteins, Cfm1 and Cfm2, are essential for the formation of cartilaginous skeletal elements.
Koji Mizuhashi (2014)
10.1371/journal.pone.0063661
Actin Microfilament Mediates Osteoblast Cbfa1 Responsiveness to BMP2 under Simulated Microgravity
Zhongquan Dai (2013)
10.1007/s10439-008-9562-4
High-Throughput Screening for Modulators of Mesenchymal Stem Cell Chondrogenesis
A. Huang (2008)
10.1089/ten.TEA.2015.0491
Initiation of Chondrocyte Self-Assembly Requires an Intact Cytoskeletal Network.
J. K. Lee (2016)
Role of the chondrocyte cytoskeleton in health and disease
Rebecca Jane Shuttleworth (2010)
10.1093/cvr/cvu134
NEXN inhibits GATA4 and leads to atrial septal defects in mice and humans.
F. Yang (2014)
10.1002/term.234
Skeletal tissue engineering using embryonic stem cells
Jojanneke M. Jukes (2010)
Enhancing mesenchymal stem cell chondrogenesis for cartilage tissue engineering
A. Huang (2010)
10.1016/j.acthis.2013.04.002
Actin is required for cellular death.
D. Grzanka (2013)
10.1007/978-981-287-152-7_8
Hydrogels for Stem Cell Fate Control and Delivery in Regenerative Medicine
W. Toh (2015)
10.1016/j.scr.2015.02.006
Hydrostatic pressure promotes the proliferation and osteogenic/chondrogenic differentiation of mesenchymal stem cells: The roles of RhoA and Rac1.
Yin-Hua Zhao (2015)
10.1002/term.125
Size of the embryoid body influences chondrogenesis of mouse embryonic stem cells
Joseph M Messana (2008)
10.1002/adhm.201200119
Surface bound amine functional group density influences embryonic stem cell maintenance.
F. Harding (2013)
The development of glycosaminoglycan-based materials to promote chondrogenic differentiation of mesenchymal stem cells
J. J. Lim (2012)
The Synergistic Effect of Bone Graft Substitute Architecture and Mechanical Environment on hMSCs Responses in vitro
F. Yang (2019)
10.22203/ECM.V019A01
Modelling condensation and the initiation of chondrogenesis in chick wing bud mesenchymal cells levitated in an ultrasound trap.
G. Edwards (2010)
10.1101/2020.01.08.897645
Over-Confluence of expanded bone marrow mesenchymal stem cells ameliorates their chondrogenic capacity in 3D cartilage tissue engineering
D. Tucker (2020)
10.1089/scd.2015.0312
Differential Regulation of SOX9 Protein During Chondrogenesis of Induced Pluripotent Stem Cells Versus Mesenchymal Stromal Cells: A Shortcoming for Cartilage Formation.
S. Diederichs (2016)
10.1007/978-3-319-29568-8_11
Cartilage Differentiation and the Actin Cytoskeleton
F. Beier (2016)
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