Online citations, reference lists, and bibliographies.
← Back to Search

Cytotoxicity Suppression And Cellular Uptake Enhancement Of Surface Modified Magnetic Nanoparticles.

A. Gupta, Mona Gupta
Published 2005 · Medicine, Materials Science

Cite This
Download PDF
Analyze on Scholarcy
Share
The aim of this study was to modify the surfaces of superparamagnetic iron oxide nanoparticles (SPION) with pullulan in order to reduce the cytotoxicity and enhance the cellular uptake of the nanoparticles. In this study, we have prepared and characterised the pullulan coated superparamagnetic iron oxide nanoparticles (Pn-SPION) of size around 40-45 nm with magnetite inner core and hydrophilic outer shell of pullulan. We have investigated the effect of cellular uptake of uncoated and Pn-SPION on cell adhesion/viability, cytotoxicity, morphology and cytoskeleton organisation of human fibroblasts. Cell cytotoxicity/adhesion studies of SPIONs on human dermal fibroblasts showed that the particles are toxic and their internalisation resulted in disruption of cytoskeleton organisation of cells. On the other hand, Pn-SPIONs were found to be non-toxic and induced changes in cytoskeleton organisation different from that observed with SPION. Transmission electron microscopy results indicated that the SPION and Pn-SPION were internalised into cells via different mechanisms, thereby suggesting that the particle endocytosis behaviour is dependent on the surface characteristics of the nanoparticles.
This paper references
10.1023/B:JMSM.0000021126.32934.20
Surface modified superparamagnetic nanoparticles for drug delivery: Interaction studies with human fibroblasts in culture
A. Gupta (2004)
10.1021/ED072P649
Determining iron content in foods by spectrophotometry
P. E. Adams (1995)
10.1016/S0168-3659(00)00368-0
Evidence for receptor-mediated hepatic uptake of pullulan in rats.
Y. Kaneo (2001)
10.1109/TNB.2003.820279
Receptor-mediated targeting of magnetic nanoparticles using insulin as a surface ligand to prevent endocytosis
A. Gupta (2003)
Long-circulating and target-specific nanoparticles: theory to practice.
S. Moghimi (2001)
10.1016/S0142-9612(03)00237-0
Dextran and albumin derivatised iron oxide nanoparticles: influence on fibroblasts in vitro.
C. Berry (2003)
10.1091/MBC.E02-05-0262
Novel proteins linking the actin cytoskeleton to the endocytic machinery in Saccharomyces cerevisiae.
H. Dewar (2002)
10.1016/0169-409X(95)00033-4
Long-circulating iron oxides for MR imaging
R. Weissleder (1995)
Article in Press
William B. Godwin (2000)
10.1016/S0092-8674(00)80461-4
Molecular Mechanisms of Endocytosis
H. Riezman (1997)
10.1002/JBM.820210202
Protein adsorption to polymer particles: role of surface properties.
D. Absolom (1987)
10.1016/S0092-8674(00)81017-X
Integrators of the Cytoskeleton that Stabilize Microtubules
Yanmin Yang (1999)
10.1034/J.1600-0854.2000.010112.X
Membrane dynamics in endocytosis: structure--function relationship.
R. Gagescu (2000)
10.1016/S0092-8674(00)80988-5
Membrane Dynamics in Endocytosis
M. Robinson (1996)
10.1023/A:1016037225728
Liver Targeting of Interferon Through Pullulan Conjugation
K. Xi (2004)
Lactoferrin and ceruloplasmin derivatized superpara-magnetic iron oxide nanoparticles: preparation, characterization and their influence on human dermal fibroblasts in culture
AK Gupta (2003)
10.1016/J.JCONREL.2003.11.006
Effect of cellular uptake of gelatin nanoparticles on adhesion, morphology and cytoskeleton organisation of human fibroblasts.
A. Gupta (2004)
10.1016/0022-1759(83)90303-4
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
10.1016/0955-0674(94)90091-4
Integrin-ligand interactions: a year in review.
T. A. Haas (1994)
10.1109/TNB.2003.820277
Surface-modified superparamagnetic nanoparticles for drug delivery: preparation, characterization, and cytotoxicity studies
A. Gupta (2004)
10.1007/978-1-4757-4064-6
Integrin-Ligand Interaction
J. Eble (1997)
Cell adhesion to biomaterials: correlations between surface charge, surface roughness, adsorbed protein, and cell morphology.
N. J. Hallab (1995)
10.1016/J.BIOMATERIALS.2003.09.095
Lactoferrin and ceruloplasmin derivatized superparamagnetic iron oxide nanoparticles for targeting cell surface receptors.
A. Gupta (2004)
Functionalisation of magnetic nanoparticles for applications in biomedicine : Biomedical applications of magnetic nanoparticles
C. Berry (2003)
10.1074/jbc.272.36.22538
Integrin-mediated Activation of Focal Adhesion Kinase Is Independent of Focal Adhesion Formation or Integrin Activation
S. Lyman (1997)
10.1016/0169-409X(95)00025-3
Long circulating microparticulate drug carriers
S. Stolnik (1995)
Pullulan and its applications.
S. Yuen (1974)
10.3109/02652049609026013
Development of superparamagnetic nanoparticles for MRI: effect of particle size, charge and surface nature on biodistribution.
C. Chouly (1996)



This paper is referenced by
10.1002/JBM.A.31022
Biocompatible superparamagnetic iron oxide nanoparticle dispersions stabilized with poly(ethylene glycol)-oligo(aspartic acid) hybrids.
S. Wan (2007)
Mammalian and bacterial toxicity of nanoparticles used in hazardous waste treatment and environmental remediation
Thomas C. Long (2007)
10.1016/j.addr.2009.03.007
Magnetic nanoparticles for theragnostics.
V. Shubayev (2009)
Curcumin Nanoconjugates-A Restitutive Therapeutics
M. P. Kusuma (2019)
10.1021/acs.molpharmaceut.5b00992
Effects of the Microparticle Shape on Cellular Uptake.
Yuanzu He (2016)
10.19261/CJM.2013.08(2).03
A REVIEW OF THE BIOGENESIS OF IRON NANOPARTICLES USING MICROORGANISMS AND THEIR APPLICATIONS
Lilia Anghel (2013)
10.1039/C1JM11370D
Multifunctional Fe3O4 nanoparticles for targeted bi-modal imaging of pancreatic cancer
C. I. Olariu (2011)
10.3109/07388551.2010.483460
Mitigating the looming vaccine crisis: production and delivery of plasmid-based vaccines
Clarence M. Ongkudon (2011)
10.1161/ATVBAHA.112.255224
Visualization of Vascular Inflammation in the Atherosclerotic Mouse by Ultrasmall Superparamagnetic Iron Oxide Vascular Cell Adhesion Molecule-1–Specific Nanoparticles
Marta Michalska (2012)
10.1016/j.nano.2009.07.008
Development of multiple-layer polymeric particles for targeted and controlled drug delivery.
Bhanuprasanth Koppolu (2010)
10.1016/J.CAP.2006.01.048
Surface-modified magnetite nanoparticles for hyperthermia: Preparation, characterization, and cytotoxicity studies
D. Kim (2006)
10.1039/C6RA03919G
Carbonyl iron coated with a sulfobetaine moiety as a biocompatible system and the magnetorheological performance of its silicone oil suspensions
Miroslav Mrlik (2016)
10.1016/J.BIOMATERIALS.2007.01.043
Nanotoxicity of iron oxide nanoparticle internalization in growing neurons.
T. R. Pisanic (2007)
10.1016/B978-0-323-42866-8.00009-5
Magnetically based nanocarriers in drug delivery
E. Denkbaş (2016)
10.1002/ADHM.201100055
Dual-imaging enabled cancer-targeting nanoparticles.
Aniket S Wadajkar (2012)
10.1016/S1567-2719(05)16005-3
chapter 5 Synthesis, Properties and Biomedical Applications of Magnetic Nanoparticles
P. Tartaj (2006)
10.1039/B816157G
Effect of surface functionality of magnetic silica nanoparticles on the cellular uptake by glioma cells in vitro
Jingxian Yu (2009)
10.2174/157015912804143513
Revolutionary Impact of Nanodrug Delivery on Neuroscience
R. Khanbabaie (2012)
10.1016/J.JMMM.2014.10.137
Water dispersible oleic acid-coated Fe3O4 nanoparticles for biomedical applications
P. B. Shete (2015)
10.3390/nano8090637
Trends towards Biomimicry in Theranostics
M. Evangelopoulos (2018)
10.1117/12.734222
Cytotoxicity of the photoluminescent silicon nanocrystals
J. Choi (2007)
10.2147/NSA.S25515
The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions?
A. Panariti (2012)
IRON OXIDE NANOPARTICLES AND THEIR TOXICOLOGICAL EFFECTS: IN VIVO AND IN VITRO STUDIES
Szalay Brigitta (2012)
10.1039/C3TB20336K
Neuronal cells loaded with PEI-coated Fe3O4 nanoparticles for magnetically guided nerve regeneration.
M. Calatayud (2013)
10.1016/J.JMMM.2008.08.104
Cytotoxicity and GMI bio-sensor detection of maghemite nanoparticles internalized into cells
F. Blanc-Béguin (2009)
10.3390/bioengineering6020052
A Multidisciplinary Approach toward High Throughput Label-Free Cytotoxicity Monitoring of Superparamagnetic Iron Oxide Nanoparticles
Sonia Abad Tan (2019)
10.1016/J.VACUUM.2017.05.022
Evaluation of cytotoxic responses of raw and functionalized multi-walled carbon nanotubes in human breast cancer (MCF-7) cells
M. A. Siddiqui (2017)
10.1002/adhm.201700932
Iron Oxide Nanoparticles: Innovative Tool in Cancer Diagnosis and Therapy
P. Martínková (2018)
10.7314/APJCP.2014.15.20.8981
Preparation of lysine-coated magnetic Fe2O3 nanoparticles and influence on viability of A549 lung cancer cells.
Yu-Hua Ma (2014)
10.1039/B711208D
Human-related application and nanotoxicology of inorganic particles: complementary aspects
S. Choi (2008)
10.4236/JBNB.2013.41008
Nanoparticle Technology as a Double-Edged Sword: Cytotoxic, Genotoxic and Epigenetic Effects on Living Cells
M. Jennifer (2013)
10.3109/10717544.2015.1006404
Folic acid-conjugated superparamagnetic iron oxide nanoparticles for tumor-targeting MR imaging
L. Li (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar