Online citations, reference lists, and bibliographies.
← Back to Search

Cold-plasma Modification Of Oxide Surfaces For Covalent Biomolecule Attachment.

B. Larson, J. M. Helgren, S. Manolache, A. Y. Lau, M. Lagally, F. Denes
Published 2005 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
While many processes have been developed to modify the surface of glass and other oxides for biomolecule attachment, they rely primarily upon wet chemistry and are costly and time-consuming. We describe a process that uses a cold plasma and a subsequent in vacuo vapor-phase reaction to terminate a variety of oxide surfaces with epoxide chemical groups. These epoxide groups can react with amine-containing biomolecules, such as proteins and modified oligonucleotides, to form strong covalent linkages between the biomolecules and the treated surface. The use of a plasma activation step followed by an in vacuo vapor-phase reaction allows for the precise control of surface functional groups, rather than the mixture of functionalities normally produced. By maintaining the samples under vacuum throughout the process, adsorption of contaminants is effectively eliminated. This process modifies a range of different oxide surfaces, is fast, consumes a minimal amount of reagents, and produces attachment densities for bound biomolecules that are comparable to or better than commercially available substrates.
This paper references
10.1149/1.2130540
Thin Film Processes
J. Vossen (1979)
10.1021/JA9936161
Synthesis and Characterization of DNA-Modified Silicon (111) Surfaces
T. Strother (2000)
Quantitative itoring of gene expression patterns with a complementary DNA croarray
D. Shalon (1995)
10.1038/NMAT779
DNA-modified nanocrystalline diamond thin-films as stable, biologically active substrates
Wensha Yang (2002)
10.1295/POLYMJ.32.222
Plasma-Grafting of Polymers with Pendant Oligo(oxyethylene) Chains onto the Surface of a Polyaniline Electrode
K. Yamada (2000)
10.1021/AC0010633
A factorial analysis of silanization conditions for the immobilization of oligonucleotides on glass surfaces.
C. M. Halliwell (2001)
10.1126/SCIENCE.1990438
Light-directed, spatially addressable parallel chemical synthesis.
S. P. Fodor (1991)
10.1002/POC.722
The gas‐phase reactivity of epichlorohydrin with hydroxide
Grant N. Merrill (2004)
10.1016/S1369-7021(04)00122-1
Glass slides to DNA microarrays
S. Conzone (2004)
10.1093/NAR/22.24.5456
Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports.
Z. Guo (1994)
10.1038/86730
Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer
T. Hughes (2001)
Synthesis and characterization of DNA - modified silicon ( 111 ) surfa
T. C. trother
10.1063/1.1688436
Controlled deposition of picoliter amounts of fluid using an ultrasonically driven micropipette
B. Larson (2004)
Immobilized Affinity Ligand Techniques
G. T. Hermanson (1992)
10.1038/nbt0302-275
Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells
D. Wang (2002)
10.1038/13664
Maskless fabrication of light-directed oligonucleotide microarrays using a digital micromirror array
S. Singh-Gasson (1999)
10.2494/PHOTOPOLYMER.10.91
Surface Functionalization of Polymers under Cold Plasma Conditions-A Mechanistic Approach
F. Denes (1997)
10.1002/PMIC.200300600
Protein microarrays: Promising tools for proteomic research
M. Templin (2003)
Plastics Technology Handbook
M. Chanda (1987)
10.1126/science.270.5235.467
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
M. Schena (1995)
10.1093/NAR/GNG086
Impact of surface chemistry and blocking strategies on DNA microarrays.
S. Taylor (2003)



This paper is referenced by
10.1021/acsami.8b15716
Facile Preparation of Epoxide-Functionalized Surfaces via Photocurable Copolymer Coatings and Subsequent Immobilization of Iminodiacetic Acids.
Zhuangsheng Lin (2018)
10.1016/J.IJBIOMAC.2006.03.018
Stepwise modification of lysine residues of glucose oxidase with citraconic anhydride.
Shahla Mossavarali (2006)
10.1016/J.IJADHADH.2017.06.017
Glass bond adhesive strength improvement by DCSBD atmospheric-pressure plasma treatment
A. Buček (2017)
10.1016/j.ijbiomac.2019.12.167
Structural modification and functional improvement of starch nanoparticles using vacuum cold plasma.
Ranran Chang (2019)
10.1016/j.bios.2008.06.038
Thermoresponsive protein adsorption of poly(N-isopropylacrylamide)-modified streptavidin on polydimethylsiloxane microchannel surfaces.
S. Sugiura (2009)
Improvement of glass wettability using Diffuse Coplanar SurfaceBarrier Discharge and gliding arc considering aging effect
V. Štěpánová (2015)
10.1007/s11090-013-9467-3
Plasma Treatment of Glass Surfaces Using Diffuse Coplanar Surface Barrier Discharge in Ambient Air
T. Homola (2013)
10.1007/978-3-540-71123-0_114
Plasmagestützte Kollagen-Beschichtung von Implantatmaterialien zur Erhöhung der Biokompatibilität
J. Hauser (2007)
10.1002/bit.22740
SOFT‐MI: A novel microfabrication technique integrating soft‐lithography and molecular imprinting for tissue engineering applications
G. Vozzi (2010)
10.1002/PPAP.200600087
Dielectric Barrier Discharge at Atmospheric Pressure as a Tool to Deposit Versatile Organic Coatings at Moderate Power Input
P. Heyse (2007)
Interface silicium/couche organique: Maitrise des propriétés et fonctionnalisation
D. Aureau (2008)
10.1016/J.CORSCI.2007.12.011
Corrosion resistance of AISI 1018 carbon steel in NaCl solution by plasma-chemical formation of a barrier layer
F. Depenyou (2008)
10.1590/S0366-69132011000400008
Funcionalização de superfícies vítreas por plasma de SiCl4 para a retenção de compostos orgânicos
O. B. G. Assis (2011)
10.1186/s40563-018-0111-6
Improved glass bonding with plasma treatment
Åsa Lundevall (2018)
10.1016/J.JBIOTEC.2007.07.222
Organic coatings using atmospheric pressure dielectric barrier discharge: en route for a straightforward manufacture of bioactive films
P. Heyse (2007)
10.1016/J.NIMB.2009.01.160
Cell patterning on a glass surface by a mask-assisted ion implantation
Chan-Hee Jung (2009)
10.1016/J.CARBON.2015.03.015
Maskless functionalization of a carbon nanotube dot array biosensor using an ultrafine atmospheric pressure plasma jet
Tomy Abuzairi (2015)
10.1098/rsif.2010.0531
Synthesis and functionalization of superparamagnetic poly-ɛ-caprolactone microparticles for the selective isolation of subpopulations of human adipose-derived stem cells
E. R. Balmayor (2011)
Semantic Scholar Logo Some data provided by SemanticScholar