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Nanostructures For Enzyme Stabilization
Jungbae Kim, J. Grate, P. Wang
Published 2006 · Chemistry
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Recent breakthroughs in nanotechnology have made various nanostructured materials more affordable for a broader range of applications. Although we are still at the beginning of exploring the use of these materials for biocatalysis, various nanostructures have been examined as hosts for enzyme immobilization via approaches including enzyme adsorption, covalent attachment, enzyme encapsulation, and sophisticated combinations of methods. This review discusses the stabilization mechanisms behind these diverse approaches; such as confinement, pore size and volume, charge interaction, hydrophobic interaction, and multipoint attachment. In particular, we will review recently reported approaches to improve the enzyme stability in various nanostructures such as nanoparticles, nanofibers, mesoporous materials, and single enzyme nanoparticles (SENs). In the form of SENs, each enzyme molecule is surrounded with a nanometer scale network, resulting in stabilization of enzyme activity without any serious limitation for the substrate transfer from solution to the active site. SENs can be further immobilized into mesoporous silica with a large surface area, providing a hierarchical approach for stable, immobilized enzyme systems for various applications, such as bioconversion, bioremediation, and biosensors.
This paper references
Immobilized enzymes: crystals or carriers?
W. Tischer (1999)
Cubic mesoporous silica with large controllable entrance sizes and advanced adsorption properties.
J. Fan (2003)
Entrapping enzyme in a functionalized nanoporous support.
C. Lei (2002)
Catalytic activity of mesoporous silicate-immobilized chloroperoxidase
Yong-Jin Han (2002)
Design of large-pore mesoporous materials for immobilization of penicillin G acylase biocatalyst
A. Chong (2004)
Immobilization of yeast alcohol dehydrogenase on magnetic nanoparticles for improving its stability
Min-Hung Liao (2004)
Rapid and high-capacity immobilization of enzymes based on mesoporous silicas with controlled morphologies.
J. Fan (2003)
How enzymes adapt: lessons from directed evolution.
F. Arnold (2001)
Chemical modification of enzymes for enhanced functionality.
G. Desantis (1999)
The Influence of Macromolecular Crowding and Macromolecular Confinement on Biochemical Reactions in Physiological Media*
A. Minton (2001)
Life's lessons in design
P. Ball (2001)
Synthesis and characterization of nanoporous silica support for enzyme immobilization
Jong-kil Kim (2004)
Mesoporous alginate/silica biocomposites for enzyme immobilisation
T. Coradin (2003)
Crosslinking of enzymes for improved stability and performance.
C. P. Govardhan (1999)
Latex Particles with Thermo‐Flocculation and Magnetic Properties for Immobilization of α‐Chymotrypsin
J. Chen (2001)
Stabilization of proteins in confined spaces.
H. X. Zhou (2001)
Nanoporous aluminum oxide as a novel support material for enzyme biosensors.
A. Heilmann (2003)
Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution.
M. Lehmann (2001)
Enzyme immobilisation using siliceous mesoporous molecular sieves
H. H. P. Yiu (2001)
Simple synthesis of hierarchically ordered mesocellular mesoporous silica materials hosting crosslinked enzyme aggregates.
J. Lee (2005)
Enzymes and Other Proteins Entrapped in Sol-Gel Materials
D. Avnir (1994)
Protein engineering 20 years on
J. Brannigan (2002)
Development of enzyme-loaded nanoparticles: effect of pH
M. Martins (1996)
Functionalization of mesoporous silica for lipase immobilization: Characterization of the support and the catalysts
Rosa M. Blanco (2004)
Single-Enzyme Nanoparticles Armored by a Nanometer-Scale Organic/Inorganic Network
J. Kim (2003)
Nonionic Triblock and Star Diblock Copolymer and Oligomeric Surfactant Syntheses of Highly Ordered, Hydrothermally Stable, Mesoporous Silica Structures
D. Zhao (1998)
Enzymatic Peptide Synthesis in Organic Solvent with Different Zeolites as Immobilization Matrixes
G. Xing (2000)
Enzyme‐Carrying Polymeric Nanofibers Prepared via Electrospinning for Use as Unique Biocatalysts
H. Jia (2002)
Encapsulation of biomolecules in silica gels
J. Livage (2001)
Enzyme immobilization in a biomimetic silica support
H. Luckarift (2004)
The principles of enzyme stabilization. II. Increase in the thermostability of enzymes as a result of multipoint noncovalent interaction with a polymeric support.
K. Martinek (1977)
Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism
C. Kresge (1992)
Highly Efficient “Tight Fit” Immobilization of α‐Chymotrypsin in Mesoporous MCM‐41: A Novel Approach Using Precursor Immobilization and Activation
N. Fadnavis (2003)
Enzyme stabilization: recent experimental progress
C. Ó'Fágáin (2003)
Effects of confinement on protein folding and protein stability
G. Ping (2003)
Enzyme stabilization by covalent binding in nanoporous sol-gel glass for nonaqueous biocatalysis.
P. Wang (2001)
Fabrication of an Amperometric Biosensor Based on the Immobilization of Glucose Oxidase in a Modified Molecular Sieve Matrix
B. Liu (1997)
Strategy for Stabilizing Enzymes Part One: Increasing Stability of Enzymes via their Multi-Point Interaction with a Support
V. Mozhaev (1990)
Adsorption of Cytochrome c on Mesoporous Molecular Sieves: Influence of pH, Pore Diameter, and Aluminum Incorporation
A. Vinu (2004)
LIPASE-SILICONE BIOCOMPOSITES : EFFICIENT AND VERSATILE IMMOBILIZED BIOCATALYSTS
I. Gill (1999)
Mechanism-based strategies for protein thermostabilization.
V. Mozhaev (1993)
New Pulp Biobleaching System Involving Manganese Peroxidase Immobilized in a Silica Support with Controlled Pore Sizes
T. Sasaki (2001)
Immobilization of enzymes in mesoporous materials: controlling the entrance to nanospace
J. Lei (2004)
Immobilization of lipase in a mesoporous reactor based on MCM-41
Hui Ma (2004)
Immobilized enzymes in ordered mesoporous silica materials and improvement of their stability and catalytic activity in an organic solvent
H. Takahashi (2001)
Peptide Synthesis Catalyzed by Crosslinked α-Chymotrypsin in Water/Dimethylformamide Solvent System
Václav C˘er˘ovský (1994)
Studies of effects of macromolecular crowding and confinement on protein folding and protein stability
Guanghui Ping (2004)
A new support for the immobilization of penicillin acylase
Jing He (2000)
Cross-linked enzyme aggregates: a simple and effective method for the immobilization of penicillin acylase.
L. Cao (2000)
Adsorption of Lysozyme over Mesoporous Molecular Sieves MCM-41 and SBA-15: Influence of pH and Aluminum Incorporation
A. Vinu (2004)
Characterization of immobilization of an enzyme in a modified Y zeolite matrix and its application to an amperometric glucose biosensor.
B. Liu (1997)
Amperometric biosensor based on a nanoporous ZrO2 matrix
B. Liu (2003)
Protein encapsulation in mesoporous silicate: the effects of confinement on protein stability, hydration, and volumetric properties.
R. Ravindra (2004)
Enzymes for chemical synthesis
K. M. Koeller (2001)
Entrapment of biocatalysts in hydrophobic sol‐gel materials for use in organic chemistry
M. Reetz (1997)
Enzyme activation for nonaqueous media.
M. Lee (2002)
Encapsulation of enzymes in mesoporous host materials via the nonsurfactant-templated sol–gel process
Yen Wei (2000)
Enzyme immobilization in reactive nanoparticles produced by inverse microemulsion polymerization
C. Daubresse (1996)
Biocatalytic plastics as active and stable materials for biotransformations
Ping Wang (1997)
Catalytic Activity in Organic Solvents and Stability of Immobilized Enzymes Depend on the Pore Size and Surface Characteristics of Mesoporous Silica
H. Takahashi (2000)
Enzyme Multilayers on Colloid Particles: Assembly, Stability, and Enzymatic Activity
F. Caruso (2000)
Crosslinked enzyme crystals
M. Navia (1997)
A new family of mesoporous molecular sieves prepared with liquid crystal templates
J. S. Beck (1992)
MESOCELLULAR SILICEOUS FOAMS WITH UNIFORMLY SIZED CELLS AND WINDOWS
Patrick Schmidt-Winkel (1999)
Synthesis and self-assembly of organoclay-wrapped biomolecules.
A. Patil (2004)
Preparation of biocatalytic nanofibres with high activity and stability via enzyme aggregate coating on polymer nanofibres.
B. Kim (2005)
Cross-linked enzyme aggregates with enhanced activity: application to lipases
P. López-Serrano (2004)
Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles.
Ansil Dyal (2003)
Enzyme encapsulation in nanoporous silica spheres.
Y. Wang (2004)
A novel method for enzyme immobilization: direct encapsulation of acid phosphatase in nanoporous silica host materials.
Y. Wei (2001)
Bio-doped Nanocomposite Polymers: Sol-Gel Bioencapsulates
I. Gill (2001)
Enzyme immobilization in MCM-41 molecular sieve
J. F. Diaz (1996)
Protease‐Containing Silicates as Active Antifouling Materials
J. Kim (2002)
Industrial biocatalysis today and tomorrow
A. Schmid (2001)
Improving enzymes by using them in organic solvents
Alexander M. Klibanov (2001)
Catalytic behaviors of enzymes attached to nanoparticles: the effect of particle mobility
H. Jia (2003)
PH CONTROL OF THE CATALYTIC ACTIVITY OF CROSS-LINKED ENZYME CRYSTALS IN ORGANIC SOLVENTS
K. Xu (1996)
This paper is referenced by
Synthesis of mesoporous silica templated by Pluronic F68 and its application in the immobilization of lipase
G. Bai (2010)
Reversible lysozyme immobilization onto N,N′-bis-(3-(4-morpholino)-propyl)-3,4,9,10-perylenetetracarboxylic acid dimide (MPPDI) attached polymeric nanospheres
N. Öztürk (2012)
Chitosan for biomedical applications
A. Abbas (2010)
Nanobiocatalysis for protein digestion in proteomic analysis
Jungbae Kim (2010)
Synthesis, characterization and catalytic activity of highly ordered hexagonal and cubic composite monoliths.
S. El-Safty (2008)
Współczesne możliwości stosowania nanotechnologii w doskonaleniu katalizy enzymatycznej
Małgorzata Lewandowska (2014)
Direct electrochemistry and electrocatalysis of myoglobin immobilized in calcium alginate–graphene microsphere films
Chong Yu (2017)
Application of Nanostructures and New Nano particles as Advanced Biomaterials
Surinder Singh Bhinder (2009)
A Review: Potential Usage of Cellulose Nanofibers (CNF) for Enzyme Immobilization via Covalent Interactions
Safwan Sulaiman (2014)
Production of Galactooligosaccharides Using β-Galactosidase Immobilized on Chitosan-Coated Magnetic Nanoparticles with Tris(hydroxymethyl)phosphine as an Optional Coupling Agent
Su-Ching Chen (2015)
Crystallin Nanofibrils: A Functionalizable Nanoscaffold with Broad Applications Manufactured from Waste.
M. Kaur (2015)
A simple fabrication of Ag-nanowires@TiO2 core-shell nanostructures for the construction of mediator-free biosensor
H. Liu (2014)
Chapter 2 Immobilization of Enzymes : A Literature Survey
B. Brena (2013)
In vitro stimulation of MC3T3-E1cells and sustained drug delivery by a hierarchical nanostructured SiO2CaOP2O5 scaffold
M. L. Ramiro-Gutiérrez (2016)
Highly active enzymes immobilized in large pore colloidal mesoporous silica nanoparticles
Dorothée Gößl (2019)
Gold Nanoparticles Stabilized in β‐Cyclodextrin and Decorated with Laccase Applied in the Construction of a Biosensor for Rutin
Patrícia Brugnerotto (2017)
Mannose based polymeric nanoparticles for lectin separation
Esra Feyzioğlu Demir (2018)
Immobilization of Laccase for Oxidative Coupling of Trans-Resveratrol and Its Derivatives
H. Zhang (2012)
Application of magnetic iron oxide nanoparticles in stabilization process of biological molecules
M. H. Salmani (2017)
Activity measurement of single enzyme nanoparticles using acrylamide layer
Nagy Endre (2011)
Mn-Doped ZnS Quantum dots–An Effective Nanoscale Sensor
J. Patel (2020)
Immobilization of Cellulases Produced by Penicillium brevicompactum AUMC 10987, using Cross-Linkage, Chitosan-Coating and Encapsulation
M. Abdel-Sater (2019)
Very stable silica-gel-bound laccase biocatalysts for the selective oxidation in continuous systems.
A. Rekuć (2010)
Magnetically-Separable and Highly-Stable Enzyme System Based on Crosslinked Enzyme Aggregates Shipped in Magnetite-Coated Mesoporous Silica
Jinwoo Lee (2009)
Toward cell‐free biofuel production: Stable immobilization of oligomeric enzymes
J. Grimaldi (2014)
Nanosized NiO particles wrapped into uniformly mesocaged silica frameworks as effective catalysts of organic amines
S. El-Safty (2008)
Multifunctional nanosystems at the interface of physical and life sciences
W. Suh (2009)
Microencapsulation of bioactive nanoparticles.
Fei Gao (2011)
Enzymes immobilized on carbon nanotubes.
W. Feng (2011)
Potential applications of enzymes immobilized on/in nano materials: A review.
S. Ansari (2012)
From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly
Nyote J. Calixte (2014)
Removal of reactive dyes using organofunctionalized mesoporous silicas
Andréa S. O. Moscofian (2013)See more