← Back to Search
Preparation And Structural Characterization Of A Partially Depolymerized Beta-glucan Obtained From Poria Cocos Sclerotium By Ultrasonic Treatment
J. Chen, L. Chen, Shaoling Lin, Chaoran Liu, P. C. Cheung
Published 2015 · Chemistry
Save to my Library
Download PDFAnalyze on Scholarcy
Abstract A water-insoluble polysaccharide (PCS) obtained by alkaline extraction from the sclerotium of a medicinal mushroom, Poria cocos (PC) was partially depolymerized by ultrasonication for various period of times up to 90 min. The untreated polysaccharide (PCS0) and two mushroom polysaccharides treated for 10 and 90 min (PCS10 and PCS90) were purified by membrane ultrafiltration. PCS90 as a hydrocolloid that had a lower intrinsic viscosity (about 30% of PCS0) and Mw (43 kDa) than PCS0. The 13C NMR spectra of PCS0 and PCS90 indicated that they both had a linear (1 → 3)-β-D-glucan which was further confirmed by methylation analysis. TEM results also showed that ultrasonic treatment could open up the compact structure of PCS into linear chain. PCS90 showed stronger inhibitory effects towards the proliferation of colorectal cancer cell SW620 than PCS0 (IC50 of PCS90 and PCS0 was 32.2 and 169.4 μg/mL, respectively). These results suggested that ultrasonic treatment might be an effective physical method for improving the solution properties of water-insoluble homoglycans such as β-glucan without changing its primary chemical structure and such modified polysaccharides had enhanced their antitumor activity.
This paper references
Ultrasonic treatment for improved solution properties of a high-molecular weight exopolysaccharide produced by a medicinal fungus.
Z. Wang (2010)
Chain conformation and anti-tumor activities of phosphorylated (1→3)-β-d-glucan from Poria cocos
X. Chen (2009)
Cell wall structure of mushroom sclerotium (Pleurotus tuber-regium): Part 2. Fine structure of a novel alkali-soluble hyper-branched cell wall polysaccharide
L. Chen (2014)
Chemical constituents and pharmacological properties of Poria cocos.
J. Ríos (2011)
Immunopotentiation and anti-tumor activity of carboxymethylated-sulfated beta-(1-->3)-d-glucan from Poria cocos.
Xiaoyu Chen (2010)
Rapid purification of polysaccharides using novel radial flow ion-exchange by response surface methodology from Ganoderma lucidum
Huiyan Jiang (2012)
Comparison of classical and ultrasound-assisted extraction of polysaccharides from Salvia officinalis L.
Z. Hromádková (1999)
Activation of the innate immune receptor Dectin-1 upon formation of a “phagocytic synapse”
H. Goodridge (2011)
Cell wall structure of mushroom sclerotium (Pleurotus tuber regium): Part 1. Fractionation and characterization of soluble cell wall polysaccharides
L. Chen (2014)
Protein-bound polysaccharide from Phellinus linteus induces G2/M phase arrest and apoptosis in SW480 human colon cancer cells.
Ge Li (2004)
Chemical components and molecular mass of six polysaccharides isolated from the sclerotium of Poria cocos.
Y. Wang (2004)
Xyloglucan degradation using different radiation sources: a comparative study.
M. Vodenicarova (2006)
THE EFFECT OF CHAIN LENGTH ON THE INTRINSIC VISCOSITY – TEMPERATURE COEFFICIENT OF LINEAR HIGH POLYMERS
L. H. Cragg (1949)
Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum
L. Zhao (2010)
Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids.
L. C. Green (1982)
Ultrasonic degradation, purification and analysis of structure and antioxidant activity of polysaccharide from Porphyra yezoensis Udea
Cunshan Zhou (2012)
Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides
S. Wasser (2002)
New method for quantitative determination of uronic acids.
N. Blumenkrantz (1973)
Effect of Molecular Mass on Antitumor Activity of Heteropolysaccharide from Poria cocos
L. Zhang (2005)
Polysaccharides in fungi. XXVI. Two branched (1----3)-beta-D-glucans from hot water extract of Yu ĕr.
T. Kiho (1991)
Correlation of structure to antitumor activities of five derivatives of a beta-glucan from Poria cocos sclerotium.
Y. Wang (2004)
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
Pharmacological, structural, and drug delivery properties and applications of 1,3-β-glucans.
Benjamin C. Lehtovaara (2011)
Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity
Mei Zhang (2007)
Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricus bisporus)
Yuting Tian (2012)
Extract of Pleurotus pulmonarius Suppresses Liver Cancer Development and Progression through Inhibition of VEGF-Induced PI3K/AKT Signaling Pathway
Wenwen Xu (2012)
Antiproliferative and differentiating effects of polysaccharide fraction from fu-ling (Poria cocos) on human leukemic U937 and HL-60 cells.
Yue-Yawn Chen (2004)
Differential pathways regulating innate and adaptive antitumor immune responses by particulate and soluble yeast-derived β-glucans.
C. Qi (2011)
Determining the polysaccharide composition of plant cell walls
F. Pettolino (2012)
Correlation between antitumor activity, molecular weight, and conformation of lentinan.
L. Zhang (2005)
A 13C nuclear magnetic resonance study of gel-forming (1 goes to 3)-beta-d-glucans. Evidence of the presence of single-helical conformation in a resilient gel of a curdlan-type polysaccharide 13140 from Alcaligenes faecalis var. myxogenes IFO 13140.
H. Saito (1977)
Determination of the maximum water solubility of eight native starches and the solubility of their acidic-methanol and -ethanol modified analogues.
R. Mukerjea (2007)
Molecular weight and anti-tumor activity of the water-soluble polysaccharides isolated by hot water and ultrasonic treatment from the sclerotia and mycelia of Pleurotus tuber-regium
M. Zhang (2004)
INFLUENCE OF MOLECULAR WEIGHT AND PERIODATE-MODIFICATION OF BETA -D-GLUCANS FROM PORIA COCOS SCLEROTIUM ON ANTITUMOR ACTIVITIES
Q. Ding (1998)
Mushrooms as Functional Foods
P. Cheung (2008)
Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata.
Xin Lin (2003)
Introduction to Polymers
R. Young (1983)
Characterization and antioxidant activities of degraded polysaccharides from Poria cocos sclerotium.
J. Tang (2014)
Evaluation of sulfated fungal beta-glucans from the sclerotium of Pleurotus tuber-regium as a potential water-soluble anti-viral agent.
Mei Zhang (2004)
Ultrasonic degradation of schizophyllan, an antitumor polysaccharide produced by Schizophyllum commune Fries.
K. Tabata (1981)
Carboxymethylated β-glucans from mushroom sclerotium of Pleurotus tuber-regium as novel water-soluble anti-tumor agent
Minxiao Zhang (2004)
Natural and Modified (1→3)-β-D-Glucans in Health Promotion and Disease Alleviation
Djordje Zekovic (2005)
Biosynthesis of (1-->3)-beta-D-glucan (callose) by detergent extracts of a microsomal fraction from Arabidopsis thaliana.
J. L. K. Him (2001)
Use of stimulatory agents to enhance the production of bioactive exopolysaccharide from pleurotus tuber-regium by submerged fermentation.
B. Zhang (2011)
Colorimetric Method for Determination of Sugars and Related Substances
M. DuBois (1956)
Mechanochemical degradation kinetics of high-density polyethylene melt and its mechanism in the presence of ultrasonic irradiation.
Y. Li (2005)
Structure and properties of a (1→3)-β-D-glucan from ultrasound-degraded exopolysaccharides of a medicinal fungus.
X. Chen (2014)
Protein determination using bicinchoninic acid in the presence of sulfhydryl reagents.
H. Hill (1988)
(1→3)-β-d-Glucans as biological response modifiers: a review of structure-functional activity relationships
John A. Bohn (1995)
This paper is referenced by
Anti-inflammatory activity of polysaccharide from Schizophyllum commune as affected by ultrasonication.
B. Du (2016)
Steady shear flow properties of Cordia myxa leaf gum as a function of concentration and temperature.
M. Chaharlang (2015)
Simple and effective purification approach to dissociate mixed water-insoluble α- and β-D-glucans and its application on the medicinal mushroom Fomitopsis betulina.
Liana Inara de Jesus (2018)
Prospects of Poria cocos polysaccharides: Isolation process, structural features and bioactivities
Xuejing Jia (2016)
Effect of β-Glucan Stabilized Virgin Coconut Oil Nanoemulsion on Properties of Croaker Surimi Gel
Asir Gani (2019)
Characterization, antioxidant and antitumor activities of polysaccharides from purple sweet potato.
Qiongying Wu (2015)
Recent Advances on Application of Ultrasound and Pulsed Electric Field Technologies in the Extraction of Bioactives from Agro-Industrial By-products
B. Kumari (2017)
Use of random forest analysis to quantify the importance of the structural characteristics of beta-glucans for prebiotic development
K. Lam (2020)
Ultrasound-assisted extraction of β-d-glucan from hull-less barley: Assessment of physicochemical and functional properties.
Abdollah Hematian Sourki (2017)
Natural polysaccharides with different conformations: extraction, structure and anti-tumor activity.
Fengzhi Lyu (2020)
A novel color image encryption algorithm based on a fractional-order discrete chaotic neural network and DNA sequence operations
L. Chen (2020)
Natural polysaccharides experience physiochemical and functional changes during preparation: A review.
Yang Yi (2020)
Purification, antitumor and anti-inflammation activities of an alkali-soluble and carboxymethyl polysaccharide CMP33 from Poria cocos.
X. Liu (2019)
Effect of the Modifications on the Physicochemical and Biological Properties of β-Glucan—A Critical Review
H. Yuan (2019)
Preparation, characterization, and α-glycosidase inhibition activity of a carboxymethylated polysaccharide from the residue of Sarcandra glabra (Thunb.) Nakai.
W. Liu (2017)
Advanced analysis of polysaccharides, novel functional components in food and medicine dual purposes Chinese herbs
J. Zhao (2017)
Hepatoprotective and Antioxidant Effects of Total Triterpenoids from Poria cocos
Yihai Wang (2017)
Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.) S. Y. Hu F
C. Hu (2020)
Immunomodulatory Activity of Polysaccharide-Protein Complex from the Mushroom Sclerotia of Polyporus rhinocerus in Murine Macrophages.
Chaoran Liu (2016)