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Evaluation Of Eudragit-coated Chitosan Microparticles As An Oral Immune Delivery System.

Mika Hori, H. Onishi, Y. Machida
Published 2005 · Chemistry, Medicine

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Chitosan microparticles containing ovalbumin (OVA), OVA-containing chitosan microparticles (Chi-OVA), were prepared, coated with Eudragit L100 (ER), and evaluated as oral vaccine. Chi-OVA with an OVA content of 34.4% (w/w) and a mean particle size of 2.3 microm were used for experiments in vitro and in vivo. ER-coated Chi-OVA (ER-Chi-OVA) contained 3.6-20.5% (w/w) OVA and had a particle size of 47.9-161.1 microm. Chi-OVA dissolved readily in JP 14 first fluid, but not in JP 14 second fluid. The release of OVA from Chi-OVA was suppressed extensively in JP 14 second fluid. ER-Chi-OVA did not dissolve in JP 14 first fluid, and the release of OVA was suppressed greatly in JP 14 first and second fluids. OVA solution, Chi-OVA and ER-Chi-OVA (200 and 800 microg OVA/mouse) were administered to Balb/C mice twice at a 1-week interval. At 7 d after the second administration, plasma OVA-specific IgG and fecal OVA-specific IgA levels were measured. OVA-specific IgG tended to be enhanced in Chi-OVA and ER-Chi-OVA, but was the highest in OVA solution. OVA-specific IgA was induced significantly more efficiently by ER-Chi-OVA than the others. These suggested that ER-Chi-OVA should be possibly useful to induce an intestinal mucosal immune response.
This paper references
10.1042/BJ1760429
Ovalbumin digestion by human pepsins 1, 3 and 5.
V. Walker (1978)
10.1016/S0168-3659(03)00093-2
Application of polyethyleneglycol (PEG)-modified liposomes for oral vaccine: effect of lipid dose on systemic and mucosal immunity.
Seiichiro Minato (2003)
10.1248/BPB.17.1272
Oral delivery of poly(lactide-co-glycolide) microspheres containing ovalbumin as vaccine formulation: particle size study.
T. Uchida (1994)
10.1080/1061186021000038319
Transport of Chitosan Microparticles for Mucosal Vaccine Delivery in a Human Intestinal M-cell Model
I. M. van der Lubben (2002)
Towards a multipurpose mucosal vaccination using phosphorylcholine as a unique antigen
S. Trollé (1998)
10.1016/0168-3659(90)90133-E
Controlled vaccine release in the gut-associated lymphoid tissues. I. Orally administered biodegradable microspheres target the peyer's patches
J. Eldridge (1990)
10.1248/BPB.26.969
Comparative study of in vitro digestibility of food proteins and effect of preheating on the digestion.
K. Takagi (2003)
10.1016/S0264-410X(02)00288-8
Polyacryl starch microparticles as adjuvant in oral immunisation, inducing mucosal and systemic immune responses in mice.
L. Wikingsson (2002)
10.1248/BPB.25.1498
Prolonged intestinal absorption of cephradine with chitosan-coated ethylcellulose microparticles in rats.
Junko Takishima (2002)
10.1016/S0264-410X(00)00094-3
Characterization of mucoadhesive microspheres for the induction of mucosal and systemic immune responses.
J. Kunisawa (2000)
10.1023/A:1018987404751
Dose and Load Studies for Subcutaneous and Oral Delivery of Poly(lactide-co-glycolide) Microspheres Containing Ovalbumin
T. Uchida (2004)
10.1016/S0264-410X(96)00149-1
Size effect on systemic and mucosal immune responses induced by oral administration of biodegradable microspheres.
Y. Tabata (1996)
10.1016/S0928-0987(01)00172-5
Chitosan and its derivatives in mucosal drug and vaccine delivery.
I. M. van der Lubben (2001)
10.1016/S0142-9612(00)00231-3
Chitosan microparticles for oral vaccination: preparation, characterization and preliminary in vivo uptake studies in murine Peyer's patches.
I. M. van der Lubben (2001)
10.1023/A:1016009313548
Enteral Absorption of Insulin in Rats from Mucoadhesive Chitosan-Coated Liposomes
H. Takeuchi (2004)
10.1016/0264-410X(89)90156-4
Microparticles as potentially orally active immunological adjuvants.
D. O'hagan (1989)
10.3109/10611860108995631
In Vivo Uptake of Chitosan Microparticles by Murine Peyer's Patches: Visualization Studies using Confocal Laser Scanning Microscopy and Immunohistochemistry
I. M. van der Lubben (2001)
10.1081/DDC-100105182
Bioadhesive Characteristics of Chitosan Microspheres to the Mucosa of Rat Small Intestine
J. Shimoda (2001)
Potential efficacy of gelatin microspheres as a new adjuvant for oral vaccination
M. Nakamura (1998)
10.1271/BBB1961.49.1335
Investigation of Digestion and Absorption of a Dietary Protein by Using Its Antigenicity as an Index: Gastrointestinal Digestion of Ovalbumin
T. Fushiki (1985)



This paper is referenced by
10.1586/erv.09.47
Chitosan-based systems for the delivery of vaccine antigens
H. C. Arca (2009)
10.1016/J.TIFS.2005.12.011
Food protein-based materials as nutraceutical delivery systems
L. Chen (2006)
10.1201/B18096-3
Emerging Trends in Delivery of Novel Vaccine Formulations
Trinh Vo (2015)
10.1533/9781845694210.2.251
10 – Food-protein-derived materials and their use as carriers and delivery systems for active food components
M. Subirade (2008)
10.2147/IJN.S38330
Design and application of chitosan microspheres as oral and nasal vaccine carriers: an updated review
M. A. Islam (2012)
10.1177/0885328212437392
In vitro evaluation of quaternized polydimethylaminoethylmethacrylate sub-microparticles for oral insulin delivery
T. Sonia (2013)
10.1021/mp300282f
Eudragit E100 as a drug carrier: the remarkable affinity of phosphate ester for dimethylamine.
M. L. Guzmán (2012)
10.1080/10611860600849464
Microcapsules formulated in the enteric coating copolymer Eudragit L100 as delivery systems for oral vaccination against infections by gastrointestinal nematode parasites
M. Dea-Ayuela (2006)
10.3390/nano7060122
A Review of the Structure, Preparation, and Application of NLCs, PNPs, and PLNs
Qianwen Li (2017)
10.1517/17425247.3.6.747
Delivery systems and adjuvants for oral vaccines
E. Lavelle (2006)
10.1016/B978-0-12-385089-8.00007-8
Chapter 7 – Nanopharmacology
Zoraida P. Aguilar (2013)
10.1016/j.biortech.2008.01.011
Advance in chitosan hydrolysis by non-specific cellulases.
W. Xia (2008)
10.3109/02652048.2013.808278
Repeated dose toxicity study of Vibrio cholerae-loaded gastro-resistant microparticles
Y. López (2014)
10.1016/J.CARBPOL.2010.10.070
In vitro evaluation of N-(2-hydroxy) propyl-3-trimethyl ammonium chitosan for oral insulin delivery
T. A. Sonia (2011)
10.18433/J3K01M
Oral immunization against hepatitis B using bile salt stabilized vesicles (bilosomes).
A. Shukla (2008)
10.1016/j.vaccine.2009.05.091
Immunoadjuvant capacity of flagellin and mannosamine-coated poly(anhydride) nanoparticles in oral vaccination.
Hesham H. Salman (2009)
10.1016/j.ajps.2018.04.001
Cyclodextrin/chitosan nanoparticles for oral ovalbumin delivery: Preparation, characterization and intestinal mucosal immunity in mice
Muye He (2019)
10.1080/03639040601085334
Preparation and In Vitro Characteristics of Lactoferrin-loaded Chitosan Microparticles
H. Onishi (2007)
10.2147/IJN.S19561
The quest for targeted delivery in colon cancer: mucoadhesive valdecoxib microspheres
N. K. Thakral (2011)
10.1007/s11095-008-9657-5
Evaluation of Bioadhesive Capacity and Immunoadjuvant Properties of Vitamin B12-Gantrez Nanoparticles
Hesham H. Salman (2008)
10.1080/02652040801903843
Improved bioavailability of orally delivered insulin using Eudragit-L30D coated PLGA microparticles
Pratap C. Naha (2008)
10.3390/vaccines4040040
Edwardsiella tarda OmpA Encapsulated in Chitosan Nanoparticles Shows Superior Protection over Inactivated Whole Cell Vaccine in Orally Vaccinated Fringed-Lipped Peninsula Carp (Labeo fimbriatus)
Saurabh Dubey (2016)
10.1016/j.vaccine.2016.07.059
Live-vaccinia virus encapsulation in pH-sensitive polymer increases safety of a reservoir-targeted Lyme disease vaccine by targeting gastrointestinal release.
Aurélie Kern (2016)
10.1016/j.ijpharm.2008.04.003
pH-sensitive and mucoadhesive thiolated Eudragit-coated chitosan microspheres.
Jishan Quan (2008)
Microparticles for oral delivery and cell encapsulation using membrane emulsification
S. Morelli (2017)
10.1080/21645515.2017.1356952
Developments in the formulation and delivery of spray dried vaccines
Gaurav Kanojia (2017)
10.3109/1061186X.2014.888071
Cellulose acetate phthalate microparticles containing Vibrio cholerae: steps toward an oral cholera vaccine
M. Pastor (2014)
10.1016/j.vaccine.2015.07.101
Chitosan-based mucosal adjuvants: Sunrise on the ocean
Y. Xia (2015)
10.1007/s10856-010-4109-2
Eudragit S-100 entrapped chitosan microspheres of valdecoxib for colon cancer
N. K. Thakral (2010)
10.1016/j.ijbiomac.2017.10.101
Chitosan-based particulate systems for the delivery of mucosal vaccines against infectious diseases.
B. Singh (2018)
10.1016/j.ijpharm.2015.08.028
Alginate coated chitosan microparticles mediated oral delivery of diphtheria toxoid. Part A. Systematic optimization, development and characterization.
A. Shukla (2015)
10.1002/APP.36714
pH‐responsive poly(DMAPMA‐co‐HEMA)‐based hydrogels for prolonged release of 5‐fluorouracil
R. Mishra (2012)
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