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Flavonolignans From Silybum Marianum Moderate UVA-induced Oxidative Damage To HaCaT Keratinocytes.

A. Svobodová, A. Zdařilová, D. Walterová, J. Vostálová
Published 2007 · Biology, Medicine

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BACKGROUND UV radiation from sunlight is a very potent environmental risk factor in the pathogenesis of skin cancer. Exposure to UV light, especially the UVA part, provokes the generation of reactive oxygen species (ROS), which induce oxidative stress in exposed cells. Topical application of antioxidants is a successful strategy for protecting the skin against UV-caused oxidative damage. OBJECTIVE In this study, silybin (SB) and 2,3-dehydrosilybin (DS) (1-50 micromol/l), flavonolignan components of Silybum marianum, were tested for their ability to moderate UVA-induced damage. METHODS Human keratinocytes HaCaT were used as an appropriate experimental in vitro model, to monitor the effects of SB and DS on cell viability, proliferation, intracellular ATP and GSH level, ROS generation, membrane lipid peroxidation, caspase-3 activation and DNA damage. RESULTS Application of the flavonolignans (1-50 micromol/l) led to an increase in cell viability of irradiated (20 J/cm(2)) HaCaT keratinocytes. SB and DS also suppressed intracellular ATP and GSH depletion, ROS production and peroxidation of membrane lipids. UVA-induced caspases-3 activity/activation was suppressed by treatment with SB and DS. Lower concentrations of both compounds (10 micromol/l) significantly reduced cellular DNA single strand break formation. CONCLUSION Taken together, the results suggest that these flavonolignans suppress UVA-caused oxidative stress and may be useful in the treatment of UVA-induced skin damage.
This paper references
Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent.
J. Sedlak (1968)
Treatment of silymarin, a plant flavonoid, prevents ultraviolet light-induced immune suppression and oxidative stress in mouse skin.
S. Katiyar (2002)
Epicatechin and its methylated metabolite attenuate UVA-induced oxidative damage to human skin fibroblasts.
S. Basu-Modak (2003)
Chemoprotective effect of plant phenolics against anthracycline‐induced toxicity on rat cardiomyocytes. Part I. Silymarin and its flavonolignans
S. Chlopcíková (2004)
Silymarin: A Review of its Clinical Properties in the Management of Hepatic Disorders
R. Agarwal (2001)
Oxidised derivatives of silybin and their antiradical and antioxidant activity.
R. Gazak (2004)
Chemoprotective effect of plant phenolics against anthracyclineinduced toxicity on rat cardiomyocytes
Š Chlopčı́ková (2004)
Silybum marianum (Carduus marianus)
P. Morazzoni (1995)
Photochemoprevention of ultraviolet B signaling and photocarcinogenesis.
F. Afaq (2005)
Microsomal lipid peroxidation.
J. A. Buege (1978)
Protective effects
CC Huang (2005)
Silybin and silymarin--new effects and applications.
V. Křen (2005)
Natural phenolics in the prevention of UV-induced skin damage. A review.
A. Svobodová (2003)
Single cell gel electrophoresis assay: methodology and applications.
E. Rojas (1999)
Photoprotective potential of lycopene, b-carotene, vitamin E, vitamin C, and carnosic acid in UVA-irradiated humanskinfibroblast.FreeRadicBiolMed2002;32:1293—303
OffordEA (2002)
Hydroxytyrosol, a natural antioxidant from olive oil, prevents protein damage induced by long-wave ultraviolet radiation in melanoma cells.
S. D'Angelo (2005)
Chemoprotective effect of plant phenolics against anthracycline‐induced toxicity on rat cardiomyocytes Part II. caffeic, chlorogenic and rosmarinic acids
S. Chlopcíková (2004)
Activation of prodeath Bcl-2 family proteins and mitochondrial apoptosis pathway by sanguinarine in immortalized human HaCaT keratinocytes.
V. Adhami (2003)
Dual efficacy of silibinin in protecting or enhancing ultraviolet B radiation-caused apoptosis in HaCaT human immortalized keratinocytes.
S. Dhanalakshmi (2004)
Attenuation of UVA-induced damage to human keratinocytes by silymarin.
A. Svobodová (2007)
Altered apoptotic response in MCF 10A cells treated with the equine estrogen metabolite, 4-hydroxyequilenin.
Y. Li (2004)
Influence of silymarin and its flavonolignans on H(2)O(2)-induced oxidative stress in human keratinocytes and mouse fibroblasts.
A. Svobodová (2006)
Sunscreens with an absorption maximum of > or =360 nm provide optimal protection against UVA1-induced expression of matrix metalloproteinase-1, interleukin-1, and interleukin-6 in human dermal fibroblasts.
G. Vielhaber (2006)
The basal layer in human squamous tumors harbors more UVA than UVB fingerprint mutations: a role for UVA in human skin carcinogenesis.
N. Agar (2004)
Sipes IG: current protocols in toxicology
MD Maines (1998)
Cellular and molecular events leading to the development of skin cancer.
V. Melnikova (2005)
Protective effects of silymarin against photocarcinogenesis in a mouse skin model.
S. Katiyar (1997)
Anti- and prooxidant effects of chronic quercetin administration in rats.
E. Choi (2003)
Silibinin modulates UVB-induced apoptosis via mitochondrial proteins, caspases activation, and mitogen-activated protein kinase signaling in human epidermoid carcinoma A431 cells.
S. Mohan (2004)
Antioxidant activity of silybin in vivo during long-term iron overload in rats.
A. Pietrangelo (1995)
Predominant effects of Polypodium leucotomos on membrane integrity, lipid peroxidation, and expression of elastin and matrixmetalloproteinase-1 in ultraviolet radiation exposed fibroblasts, and keratinocytes.
N. Philips (2003)
The production of reactive oxygen species by dietary flavonols.
A. Canada (1990)
Beneficial effects of quercetin on oxidative stress induced by ultraviolet A
M. Erden Inal (2001)
Sunscreens inadequately protect against ultraviolet-A-induced free radicals in skin: implications for skin aging and melanoma?
R. Haywood (2003)
Photoprotective properties of Prunella vulgaris and rosmarinic acid on human keratinocytes.
J. Psotová (2006)
Protective effects of (−)-epicatechin-3-gallate on UVA-induced damage in HaCaT keratinocytes
Chieh-Chen Huang (2005)
Flavonoid antioxidants.
C. Rice-evans (2001)
Silibinin Protects against Photocarcinogenesis via Modulation of Cell Cycle Regulators, Mitogen-Activated Protein Kinases, and Akt Signaling
G. Mallikarjuna (2004)
Quercetin may act as a cytotoxic prooxidant after its metabolic activation to semiquinone and quinoidal product.
D. Metodiewa (1999)
Ultraviolet A induces apoptosis via reactive oxygen species in a model for Smith-Lemli-Opitz syndrome.
A. Valencia (2006)
Chemoprevention of photocarcinogenesis by selected dietary botanicals.
M. S. Baliga (2006)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
Influence of silymarin and its flavonolignans on doxorubicin‐iron induced lipid peroxidation in rat heart microsomes and mitochondria in comparison with quercetin
J. Psotová (2002)
Protective Effects of Cyanidin‐3‐O‐β‐glucopyranoside Against UVA‐induced Oxidative Stress in Human Keratinocytes ¶
A. Tarozzi (2005)
Protective effect of resveratrol against oxidative damage of UVA irradiated HaCaT cells.
Ming-liang Chen (2006)
Adaptive cellular protection against UVA-1-induced lipid peroxidation in human dermal fibroblasts shows donor-to-donor variability and is glutathione dependent
L. Schneider (2005)
Current protocols in toxicology
M. Maines (1999)
Caspases Mediate 6‐Hydroxydopamine‐Induced Apoptosis but Not Necrosis in PC12 Cells
E. Ochu (1998)
Photoprotective potential of lycopene, beta-carotene, vitamin E, vitamin C and carnosic acid in UVA-irradiated human skin fibroblasts.
E. Offord (2002)
Ultraviolet light induced alteration to the skin.
A. Svobodová (2006)
The green tea polyphenol, epigallocatechin‐3‐gallate, protects against the oxidative cellular and genotoxic damage of UVA radiation
S. E. Tobi (2002)
The single cell gel assay: a sensitive technique for evaluating intercellular differences in DNA damage and repair.
R. Tice (1990)
Suppression of UVA-mediated release of labile iron by epicatechin--a link to lysosomal protection.
S. Basu-Modak (2006)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)

This paper is referenced by
Tunable optical properties of silymarin flavonolignans
M. Biler (2016)
Novel cosmeceuticals from plants—An industry guided review
A. Dorni (2017)
Silymarin: An interesting modality in dermatological therapeutics
Konchok Dorjay (2018)
Protective effects of 2'-3'-dihydroxy-4',6'-dimethoxychalcone derived from green perilla leaves against UV radiation-induced cell injury in human cultured keratinocytes.
Yuki Takada-Takatori (2019)
Oxidative Stress and Chronic Degenerative Diseases - A Role for Antioxidants
José A. Morales-González (2013)
Chemoprevention of Skin Cancer by Natural Compounds 199 increasing oxidative stress and skin damage
G. Filip (2012)
Silymarin attenuates benzo(a)pyrene induced toxicity by mitigating ROS production, DNA damage and calcium mediated apoptosis in peripheral blood mononuclear cells (PBMC).
Kiruthiga Perumal Vijayaraman (2012)
The dermato-protective effects of lucidone from Lindera erythrocarpa through the induction of Nrf2-mediated antioxidant genes in UVA-irradiated human skin keratinocytes
You-Cheng Hseu (2015)
Effects of Chlorophyll a on UVB-induced Cellular Responses and Type I pN Collagen Synthesis in vitro
H. Jeon (2009)
Silymarin in Dermatology: A Brief Review
S. Mehraban (2014)
Hepatoprotective activity of the aerial parts of Caralluma dalzielii N. E. Brown against carbon tetrachloride -induced hepatotoxicity in rats
C. J. Ugwah-Oguejiofor (2018)
Methods for evaluation of cosmetic antioxidant capacity
Anna Ratz-Lyko (2012)
Protective effects of jca 3000 + CP against ultraviolet-induced damage in HaCaT and MEF cells
S. Ren (2010)
Metal coordination protocol for the synthesis of-2,3-dehydrosilybin and 19-O-demethyl-2,3-dehydrosilybin from silybin and their antitumor activities
Yong-ju Wen (2018)
12 Silymarin , Natural Flavonolignans from Milk Thistle
S. AbouZid (2012)
Protective effects of kappa-ca3000+CP against ultraviolet-induced damage in HaCaT and MEF cells.
S. Ren (2010)
Ultraviolet A protective potential of plant extracts and phytochemicals.
Denisa Skarupova (2020)
Molecular insights in tracking optical properties and antioxidant activities of polyphenols
M. Biler (2017)
The protective effects of the antioxidant combination of ferulic acid with vitamins C and E against UV-induced photodamage in human skin cells
Emma Lewis Bone (2010)
In Silico Identification and In Vitro Evaluation of Natural Inhibitors of Leishmania major Pteridine Reductase I
F. Herrmann (2017)
Down regulation of CD147 boosts the premature senescence in human skin fibroblasts by destroying the redox balance and inhibiting klotho.
H. Xie (2011)
Efficacy of Pre- and Post-Treatment by Topical Formulations Containing Dissolved and Suspended Silybum marianum against UVB-Induced Oxidative Stress in Guinea Pig and on HaCaT Keratinocytes
P. Fehér (2016)
Effects of heme oxygenase-1 upregulation on isoproterenol-induced myocardial infarction
Somaia A.G. Eltobshy (2019)
Molecular mechanisms of inhibition of photocarcinogenesis by silymarin, a phytochemical from milk thistle (Silybum marianum L. Gaertn.) (Review).
M. Vaid (2010)
Solid-state fermentation of industrial solid wastes from the fruits of milk thistle Silybum marianum for feed quality improvement
F. Li (2013)
The use of Eudragit RS 100/cyclodextrin nanoparticles for the transmucosal administration of glutathione.
A. Lopedota (2009)
Hepatoprotective effect of 2,3-dehydrosilybin on carbon tetrachloride-induced liver injury in rats.
Byoung-Ok Cho (2013)
Apoptosis induction is involved in UVA-induced autolysis in sea cucumber Stichopus japonicus.
Hang Qi (2016)
Poss/TiO2 as sun protection factors for skin changes caused by ultraviolet A radiation
X. Chen (2008)
The Chemoprevention of Chronic Degenerative Disease Through Dietary Antioxidants: Progress, Promise and Evidences
E. Madrigal-Santillán (2013)
Resveratrol protects human keratinocytes HaCaT cells from UVA-induced oxidative stress damage by downregulating Keap1 expression.
Y. Liu (2011)
Bioavailability of silymarin flavonolignans: drug formulations and biotransformation
Eleni Theodosiou (2013)
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