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Electrochemical Characterization Of Dehaloperoxidase Adsorbates On COOH/OH Mixed Self-assembled Monolayers

T. Chen, E. F. Bowden
Published 2013 · Chemistry

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Abstract Electrochemical characterization of Amphitrite ornata dehaloperoxidase, a monomeric hemoglobin, adsorbed on COOH/OH-terminated alkanethiolate mixed self-assembled monolayers (SAMs) is reported. Adsorption was achieved by simple electrode exposure to low ionic strength protein solution at pH 6.0, and cyclic voltammetry (CV) was employed to measure surface concentration, electron transfer rate, and formal potential under anaerobic conditions. Surface concentration values determined by CV were found to be inversely proportional to scan rate, a behavior that is different than that observed for electron transfer proteins such as cytochrome c. We attribute this behavior to a dynamic heterogeneous adsorbate layer that permits some fraction of the molecules to undergo reorientation during a CV scan. A model was proposed that classifies adsorbates among three populations with respect to their most stable orientations, namely, electroactive adsorbates, latent electroinactive adsorbates capable of undergoing reorientation to electroactive states, and electroinactive adsorbates. Electrochemical results and the proposed model are discussed in terms of the tertiary structure and surface properties of dehaloperoxidase and are compared to the docking and electron transfer reactions of the myoglobin/cytochrome b5 system. Examination of the effects of ionic strength and mixed SAM composition provide support for the proposed model.
This paper references
10.1126/science.252.5009.1164
Self-assembled organic monolayers: model systems for studying adsorption of proteins at surfaces
K. L. Prime (1991)
10.2116/ANALSCI.20.603
Third-generation biosensors based on the direct electron transfer of proteins.
W. Zhang (2004)
10.1016/j.febslet.2009.02.046
The courtship of proteins: Understanding the encounter complex
M. Ubbink (2009)
10.1021/LA701751R
pH-Induced changes in adsorbed cytochrome c. voltammetric and surface-enhanced resonance Raman characterization performed simultaneously at chemically modified silver electrodes.
D. Millo (2007)
10.1016/0022-0728(92)80402-P
Direct electron transfer of horse heart myoglobin at an indium oxide electrode
I. Taniguchi (1992)
10.1007/S11426-010-0134-8
Electron transfer and interfacial behavior of redox proteins
N. Zhou (2010)
10.1021/CR0300789
Self-assembled monolayers of thiolates on metals as a form of nanotechnology.
J. Love (2005)
10.1021/ja910707r
Molecular basis of coupled protein and electron transfer dynamics of cytochrome c in biomimetic complexes.
Damián Alvarez-Paggi (2010)
10.1016/J.ELECOM.2012.10.011
Voltammetry of dehaloperoxidase on self-assembled monolayers: Reversible adsorptive immobilization of a globin
E. L. D’Antonio (2013)
10.1149/1.2131817
Electroanalytical Chemistry: A Series of Advances
Allen J. Bard (1974)
10.1021/AC00110A010
Electron transfer from electrodes to myoglobin: facilitated in surfactant films and blocked by adsorbed biomacromolecules.
A. E. Nassar (1995)
10.1016/S1388-2481(01)00299-5
Adsorptive immobilization of cytochrome c on indium/tin oxide (ITO): electrochemical evidence for electron transfer-induced conformational changes
A. E. Kasmi (2002)
10.1172/JCI6609
Protein-protein interaction in insulin signaling and the molecular mechanisms of insulin resistance.
A. Virkamäki (1999)
10.1021/JP992591P
An electrochemical approach to investigate gated electron transfer using a physiological model system: Cytochrome c immobilized on carboxylic acid-terminated alkanethiol self-assembled monolayers on gold electrodes
A. Avila (2000)
10.1021/JA962366A
Molecular orientation distributions in protein films. 1. Cytochrome c adsorbed to substrates of variable surface chemistry
P. Edmiston (1997)
10.1074/JBC.271.9.4609
An Unusual Dehalogenating Peroxidase from the Marine Terebellid Polychaete Amphitrite ornata(*)
Y. Chen (1996)
10.1021/JP0105589
Ordered Assembly and Controlled Electron Transfer of the Blue Copper Protein Azurin at Gold (111) Single-Crystal Substrates
Qijin Chi (2001)
10.1021/JA054219V
Determination of the orientation of adsorbed cytochrome C on carboxyalkanethiol self-assembled monolayers by in situ differential modification.
J. Xu (2006)
10.1039/c0cs00056f
Immobilization of bio-macromolecules on self-assembled monolayers: methods and sensor applications.
Debasis Samanta (2011)
10.1021/JA067598G
Dynamic docking of cytochrome b5 with myoglobin and alpha-hemoglobin: heme-neutralization "squares" and the binding of electron-transfer-reactive configurations.
Korin E Wheeler (2007)
10.1016/J.JELECHEM.2011.12.015
Thin-layer spectroelectrochemistry of the Fe(III)/Fe(II) redox reaction of dehaloperoxidase-hemoglobin
E. L. D’Antonio (2012)
10.1021/ja8016895
Direct observation of the gating step in protein electron transfer: electric-field-controlled protein dynamics.
Anja Kranich (2008)
10.1002/ADFM.200600968
Template‐Synthesized Protein Macroporous Biofilms: Conformational Related Direct Electron Transfer
Yan-Yan Song (2007)
10.1021/JA00005A068
Electron-transfer reaction of cytochrome c adsorbed on carboxylic acid terminated alkanethiol monolayer electrodes
M. Tarlov (1991)
10.1016/0022-0728(96)04551-2
The distribution of standard rate constants for electron transfer between thiol-modified gold electrodes and adsorbed cytochrome c
T. M. Nahir (1996)
10.1039/c2cc35819k
Heme plane orientation dependent direct electron transfer of cytochrome c at SAMs/Au electrodes with different wettability.
G. Wang (2012)
10.1002/ADFM.200500048
Adsorption and Direct Electron Transfer from Hemoglobin into a Three‐Dimensionally Ordered Macroporous Gold Film
C. Wang (2005)
10.1021/JA993951P
Electrostatic control of electron transfer between myoglobin and cytochrome b5: Effect of methylating the heme propionates of Zn-myoglobin [16]
Z. Liang (2000)
10.1039/C2CP41949A
Interfacial hydration, dynamics and electron transfer: multi-scale ET modeling of the transient [myoglobin, cytochrome b5] complex.
Shahar Keinan (2012)
10.1016/J.JELECHEM.2010.12.020
Electric-field effects on the interfacial electron transfer and protein dynamics of cytochrome c
H. K. Ly (2011)
10.1021/JA973212D
The Unusual Reactivities of Amphitrite ornata Dehaloperoxidase and Notomastus lobatus Chloroperoxidase Do Not Arise from a Histidine Imidazolate Proximal Heme Iron Ligand
S. Franzén (1998)
10.1021/JP055768Q
The effect of ionic strength on the electron-transfer rate of surface immobilized cytochrome C.
Hongjun Yue (2006)
10.1021/J100126A037
Characterization of cytochrome c/alkanethiolate structures prepared by self-assembly on gold
Shihua. Song (1993)
10.1021/bi801916j
Characterization of dehaloperoxidase compound ES and its reactivity with trihalophenols.
Jeremiah A. Feducia (2009)
10.1016/S0022-0728(79)80075-3
General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems
E. Laviron (1979)
10.1098/rspa.1972.0083
Dielectric dispersion and dipole moment of myoglobin in water
G. South (1972)
10.1021/LA960650+
Voltammetric Peak Broadening for Cytochrome c/Alkanethiolate Monolayer Structures: Dispersion of Formal Potentials
Rose A. Clark† and (1997)
10.1021/JP065805T
Protein-protein interactions studied by EPR relaxation measurements: cytochrome c and cytochrome c oxidase.
Sevdalina Lyubenova (2007)
10.1016/s0021-9258(18)34740-9
The asymmetric distribution of charges on the surface of horse cytochrome c. Functional implications.
W. Koppenol (1982)
10.1021/JP0620670
On the electron transfer mechanism between cytochrome C and metal electrodes. Evidence for dynamic control at short distances.
Hongjun Yue (2006)
10.1021/JP303740E
Electric-field controle of the pH-dependent redox process of cytochrome c immobilized on gold electrode
B. Jin (2012)
10.1007/s00775-001-0332-0
Dynamic docking and electron transfer between myoglobin and cytochrome b5
Zhao-Xun Liang (2001)
10.1142/9789814280228_0027
27 Heme Protein-Based Electrochemical Biosensors
G. Li (2010)
10.1021/JA038163L
Dynamic docking and electron-transfer between cytochrome b5 and a suite of myoglobin surface-charge mutants. Introduction of a functional-docking algorithm for protein-protein complexes.
Zhao-Xun Liang (2004)



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