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Biosorption And Biotransformation Of Cr(VI) By Novel Cellulosimicrobium Funkei Strain AR6

Chinnannan Karthik, V. S. Ramkumar, A. Pugazhendhi, A. Pugazhendhi, K. Gopalakrishnan, K. Gopalakrishnan, P. I. Arulselvi
Published 2017 · Chemistry

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Abstract A novel Cr(VI) tolerant bacterial strain AR6 was isolated from leather industrial effluent contaminated soil sample sources and it was identified as Cellulosimicrobium funkei following 16S rDNA gene sequencing. Effect of various physicochemical properties on Cr(VI) reducing ability of the strain was also studied. The results showed that the C. funkei strain AR6 was tolerant to very high concentration of Cr(VI) (1200 µg/ml) and capable of reducing Cr(VI), under a wide range of pH (6–8) and temperature (30–40°C) with an optimum at 7.0 and 35 °C. Further, studies verified that the removal of Cr(VI) was mainly achieved by a metabolism depending on the bioreduction process. The C. funkei strain AR6 reduces and accumulates chromium both in and around the cells, with cell walls acting as the major binding sites for chromium. Raman spectroscopy analysis further confirmed that the chromium immobilization by the cells was in the Cr(III) state. Toxic nature of the reduced product was evaluated by toxicity study using zebrafish embryos ( Danio rerio ) model. Interestingly, when embryo exposed Cr(VI) survival (22.19%) and hatching (26.71%) rates were reduced significantly. However, Cr(VI) intoxicated embryos significantly increased the cardiac function (132 ± 2.65 beat/min) at 60 hpf. Conversely, reduced product excreted less teratogenic effects on zebrafish embryos. The present finding suggested that the transformation of Cr(VI) to Cr(III) by novel C. funkei is considered as an effective, ecofriendly and attractive strategy for remediation of Cr(VI) polluted sites.
This paper references
Mechanism of Cr(VI) reduction by Aspergillus niger: enzymatic characteristic, oxidative stress response, and reduction product
Y. Gu (2014)
Electron energy loss spectroscopy techniques for the study of microbial chromium(VI) reduction.
T. Daulton (2002)
Biosorption of chromium and nickel by heavy metal resistant fungal and bacterial isolates.
Shankar Congeevaram (2007)
Thiobencarb-induced embryotoxicity in medaka (Oryzias latipes): stage-specific toxicity and the protective role of chorion.
Villalobos (2000)
Experiments in biochemistry
S. O. Farrell (1999)
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.
K. Tamura (2013)
Reduction of hexavalent chromium by Pannonibacter phragmitetus LSSE-09 coated with polyethylenimine-functionalized magnetic nanoparticles under alkaline conditions.
L. Xu (2011)
Investigation on mechanism of Cr(VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil.
S. Das (2014)
Hexavalent chromium reduction by Pannonibacter phragmitetus BB isolated from soil under chromium-containing slag heap
L. Y. Chai (2009)
Biosorption of heavy metals by Bacillus thuringiensis strain OSM29 originating from industrial effluent contaminated north Indian soil.
M. Oves (2013)
Chromium reducing and plant growth promoting novel strain Pseudomonas aeruginosa OSG41 enhance chickpea growth in chromium amended soils
M. Oves (2013)
Cation exchange in cell walls of gram-positive bacteria.
R. Marquis (1976)
Chromium in the natural and human environments
J. Nriagu (1988)
Plant growth promotion by a hexavalent chromium reducing bacterial strain, Cellulosimicrobiumcellulans KUCr3
Swagata Chatterjee (2009)
Survival and chromate reducing ability of Pseudomonas aeruginosa in industrial effluents
A. Ganguli (1999)
Chemical and microbial remediation of hexavalent chromium from contaminated soil and mining/metallurgical solid waste: a review.
B. Dhal (2013)
[1] Whole genome DNA microarray expression analysis of biofilm development by Vibrio cholerae O1 El Tor
G. Schoolnik (2001)
Detoxification of metal-bearing effluents: biosorption for the next century
B. Volesky (2001)
Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent
S. Srivastava (2006)
Bergey's Manual of Determinative Bacteriology.
R. E. Buchanan (1975)
Detoxification of hexavalent chromium by an indigenous facultative anaerobic Bacillus cereus strain isolated from tannery effluent
N. Singh (2013)
Chromium-induced genotoxicity and apoptosis: relationship to chromium carcinogenesis (review).
J. Singh (1998)
Aerobic chromate reduction by Bacillus subtilis
C. Garbisu (2004)
Cometabolism of Cr(VI) by Shewanella oneidensis MR‐1 produces cell‐associated reduced chromium and inhibits growth
Sarah S Middleton (2003)
The behaviour and ecology of the zebrafish, Danio rerio
R. Spence (2008)
Characterization of clinical isolates previously identified as Oerskovia turbata: proposal of Cellulosimicrobium funkei sp. nov. and emended description of the genus Cellulosimicrobium.
June M. Brown (2006)
Pentachlorophenol dechlorination and simultaneous Cr6+ reduction by Pseudomonas putida SKG-1 MTCC (10510): characterization of PCP dechlorination products, bacterial structure, and functional groups
S. Garg (2012)
Reduction of hexavalent chromium by Bacillus sp. isolated from chromite mine soils and characterization of reduced product.
B. Dhal (2010)
Removal of cadmium from aqueous solution by batch studies using Bacillus cereus
Pugazhendhi Arivalagan (2014)
Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: a review.
H. Thatoi (2014)
Toxicity of cylindrospermopsin, and other apparent metabolites from Cylindrospermopsis raciborskii and Aphanizomenon ovalisporum, to the zebrafish (Danio rerio) embryo.
J. P. Berry (2009)
Experimental study on Cr (V) reduction by Pseudomonas aeruginosa.
Y. Liu (2004)
A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
Lv-hui Sun (2015)
Membrane-bound respiratory system of Enterobacter cloacae strain HO1 grown anaerobically with chromate.
P. C. Wang (1991)
Quantification and identification of culturable airborne bacteria from duck houses.
E. Martín (2010)
FTIR spectroscopic studies of bacterial cellular responses to environmental factors, plant-bacterial interactions and signalling
A. Kamnev (2008)
Taxifolin mitigates oxidative DNA damage in vitro and protects zebrafish (Danio rerio) embryos against cadmium toxicity.
K. Manigandan (2015)
Chromium-resistant microorganisms isolated from evaporation ponds of a metal processing plant
M. Losi (1994)
The zebrafish book : a guide for the laboratory use of zebrafish (Danio rerio)
M. Westerfield (1995)
Assessing the Phytotoxicity of Tannery Waste-Contaminated Soil on Zea mays (Lin) Growth
Muhammad Yasin (2013)
Hexavalent chromium reduction by Acinetobacter haemolyticus isolated from heavy-metal contaminated wastewater.
Zainul Akmar Zakaria (2007)
Inducible chromate reductase exhibiting extracellular activity in Bacillus methylotrophicus for chromium bioremediation.
John Geraldine Sandana Mala (2015)
Uptake and retention of metals by cell walls of Bacillus subtilis.
T. Beveridge (1976)
Cr(VI) uptake mechanism of Bacillus cereus.
Z. Chen (2012)
Cellulosimicrobium funkei-like enhances the growth of Phaseolus vulgaris by modulating oxidative damage under Chromium(VI) toxicity
C. Karthik (2016)

This paper is referenced by
Ensifer adhaerens for heavy metal bioaccumulation, biosorption, and phosphate solubilization under metal stress condition
M. Oves (2017)
Amelioration of chromium and heat stresses in Sorghum bicolor by Cr6+ reducing-thermotolerant plant growth promoting bacteria.
L. Bruno (2019)
Evaluation of Cr(VI) reduction mechanism and removal by Cellulosimicrobium funkei strain AR8, a novel haloalkaliphilic bacterium.
C. Karthik (2017)
Actinobacteria consortium as an efficient biotechnological tool for mixed polluted soil reclamation: Experimental factorial design for bioremediation process optimization.
J. D. Aparicio (2018)
Single, simultaneous and consecutive biosorption of Cr(VI) and Orange II onto chemically modified masau stones.
Ahmad B. Albadarin (2017)
Facile large scale synthesis of CuCr2O4/CuO nanocomposite using MOF route for photocatalytic degradation of methylene blue and tetracycline under visible light
S. Hariganesh (2020)
An assessment of trace element contamination in groundwater aquifers of Saharanpur, Western Uttar Pradesh, India
Amrish Kumar (2019)
Operational Characteristics of Immobilized Ochrobactrum sp. CUST210-1 Biosystem and Immobilized Chromate Reductase Biosystem in Continuously Treating Actual Chromium-Containing Wastewater
Gueyhorng Wang (2020)
Microbes and Signaling Biomolecules Against Plant Stress: Strategies of Plant- Microbe Relationships for Better Survival
Anita Sharma (2021)
Chromium(VI) bioreduction behavior and microbial revolution by phosphorus minerals in continuous flow experiment.
Linlin Ma (2020)
Natural remediation of the main effluents of trotu. river affected by heavy metals pollution
Gabriel-Alin Iosob (2019)
A review on cleaner strategies for chromium industrial wastewater: Present research and future perspective
K. GracePavithra (2019)
Biological approaches to tackle heavy metal pollution: A survey of literature.
J. Jacob (2018)
Adsorptive Removal and Recovery of Heavy Metal Ions from Aqueous Solution/Effluents Using Conventional and Non-conventional Materials
A. Gopinath (2020)
Bacterial Biosorbents and Biotransformers Stimulates Plant Growth Promotion By Activation of Defense Mechanism and Nutrient Uptake
P. A. Wani (2020)
Biosorption of heavy metal polluted soil using bacteria and fungi isolated from soil
O. A. Oyewole (2019)
Acidithiobacillus ferrooxidans enhanced heavy metals immobilization efficiency in acidic aqueous system through bio-mediated coprecipitation
G. Min (2017)
Coupling enhancement of Chromium(VI) bioreduction in groundwater by phosphorus minerals.
Linlin Ma (2019)
Isolation and identification of metal-tolerant and antibiotic-resistant bacteria from soil samples of Cachar district of Assam, India
S. Nath (2019)
Biosorption of Cr(VI) by Halomonas sp. DK4, a halotolerant bacterium isolated from chrome electroplating sludge
Vidhi N. Kalola (2019)
Prospective of chromium (VI) reduction under in vitro and in vivo conditions and stimulation of antioxidant defense of cowpea under the exposure of Cr (VI)
P. A. Wani (2018)
Characterization of multifarious plant growth promoting traits of rhizobacterial strain AR6 under Chromium (VI) stress.
C. Karthik (2017)
Design and optimization of a new reactor based on biofilm-ceramic for industrial wastewater treatment.
Ali Aghababai Beni (2019)
Gentle remediation options for soil with mixed chromium (VI) and lindane pollution: biostimulation, bioaugmentation, phytoremediation and vermiremediation
Rafael G. Lacalle (2020)
Microbial Bioremediation & Biodegradation
M. Shah (2020)
Biosorptive Removal of Cadmium(II) and Copper(II) Using Microwave-Assisted Thiourea-Modified Sorghum bicolor Agrowaste
M. Salman (2020)
Bioreduction and biosorption of Cr(VI) by a novel Bacillus sp. CRB-B1 strain.
Hang Tan (2019)
Biosorption and biotransformation of hexavalent chromium [Cr(VI)]: A comprehensive review.
Renitta Jobby (2018)
Study on influencing factors and mechanism of removal of Cr(VI) from soil suspended liquid by bentonite-supported nanoscale zero-valent iron
S. Liu (2020)
Biosorption, an efficient method for removing heavy metals from industrial effluents: A Review
Ali Aghababai Beni (2020)
Cleaning chromium pollution in aquatic environments by bioremediation, photocatalytic remediation, electrochemical remediation and coupled remediation systems
Caiwen He (2020)
Microbial reduction fate of chromium (Cr) in aqueous solution by mixed bacterial consortium.
L. Ma (2019)
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