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Localization Of Superoxide Anion Production To Mitochondrial Electron Transport Chain In 3-NPA-treated Cells.
A. Bácsi, Mitchell W Woodberry, W. Widger, J. Papaconstantinou, S. Mitra, J. Peterson, I. Boldogh
Published 2006 · Chemistry, Medicine
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3-Nitropropionic acid (3-NPA), an inhibitor of succinate dehydrogenase (SDH) at complex II of the mitochondrial electron transport chain induces cellular energy deficit and oxidative stress-related neurotoxicity. In the present study, we identified the site of reactive oxygen species production in mitochondria. 3-NPA increased O2- generation in mitochondria respiring on the complex I substrates pyruvate+malate, an effect fully inhibited by rotenone. Antimycin A increased O2- production in the presence of complex I and/or II substrates. Addition of 3-NPA markedly increased antimycin A-induced O2- production by mitochondria incubated with complex I substrates, but 3-NPA inhibited O2- formation driven with the complex II substrate succinate. At 0.6 microM, myxothiazol inhibits complex III, but only partially decreases complex I activity, and allowed 3-NPA-induced O2- formation; however, at 40 microM myxothiazol (which completely inhibits both complexes I and III) eliminated O2- production from mitochondria respiring via complex I substrates. These results indicate that in the presence of 3-NPA, mitochondria generate O2- from a site between the ubiquinol pool and the 3-NPA block in the respiratory complex II.
This paper references
Effect of bathophenanthroline and carbonylcyanide-m-chlorophenyl hydrazone on cytochrome c reductase activity of resolved succinate cytochrome c reductase complex.
B. Trumpower (1975)
3-Nitropropionate, the toxic substance of Indigofera, is a suicide inactivator of succinate dehydrogenase.
T. Alston (1977)
Inactivation of succinate dehydrogenase by 3-nitropropionate.
C. J. Coles (1979)
Studies on the stabilized ubisemiquinone species in the succinate-cytochrome c reductase segment of the intact mitochondrial membrane system.
J. Salerno (1980)
Effect of membrane potential and pH gradient on electron transfer in cytochrome oxidase.
P. M. Moroney (1984)
Effect of membrane
P. M. Moroney (1984)
Fumarate reductase and other mitochondrial activities in Trypanosoma cruzi.
A. Boveris (1986)
Triphasic reduction of cytochrome b and the protonmotive Q cycle pathway of electron transfer in the cytochrome bc1 complex of the mitochondrial respiratory chain.
H. Tang (1986)
 Fumarate reductase of Escherichia coli☆
B. Lemire (1986)
Properties of bovine heart mitochondrial cytochrome b560.
L. Yu (1987)
Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation.
M. P. King (1989)
Human cells lacking mtDNA: repopulation
The protonmotive Q cycle. Energy transduction by coupling of proton translocation to electron transfer by the cytochrome bc1 complex.
B. Trumpower (1990)
3-Nitropropionic acid-exogenous animal neurotoxin and possible human striatal toxin.
A. Ludolph (1991)
quinone oxidoreductase (complex I)
A. C. Ludolph (1991)
Dihydrorhodamine 123: a fluorescent probe for superoxide generation?
L. Henderson (1993)
Neurochemical and histologic characterization of striatal excitotoxic lesions produced by the mitochondrial toxin 3-nitropropionic acid
M. F. Beal (1993)
Evaluation of 2',7'-dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracellular H2O2 in cultured endothelial cells.
J. Royall (1993)
A redox-based O2 sensor in rat pulmonary vasculature.
S. Archer (1993)
Sequence comparison between the flavoprotein subunit of the fumarate reductase (complex II) of the anaerobic parasitic nematode, Ascaris suum and the succinate dehydrogenase of the aerobic, free-living nematode, Caenorhabditis elegans.
T. Kuramochi (1994)
T. Aoki (1994)
A metabolic enzyme that rapidly produces superoxide, fumarate reductase of Escherichia coli.
J. Imlay (1995)
Lipid peroxidation in rats intoxicated with 3-nitropropionic acid.
Y. Fu (1995)
Assessment of mitochondrial oxidative phosphorylation in patient muscle biopsies, lymphoblasts, and transmitochondrial cell lines.
I. Trounce (1996)
Succinate: quinone oxidoreductases. Variations on a conserved theme.
C. Hägerhäll (1997)
Cobalt and desferrioxamine reveal crucial members of the oxygen sensing pathway in HepG2 cells.
W. Ehleben (1997)
A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases.
M. Zhou (1997)
Mitochondrial reactive oxygen species trigger hypoxia-induced transcription.
N. Chandel (1998)
Intracellular Signaling by Reactive Oxygen Species during Hypoxia in Cardiomyocytes*
J. Duranteau (1998)
Inhibitors of NADH-ubiquinone reductase: an overview.
M. Esposti (1998)
Increased Vulnerability to 3‐Nitropropionic Acid in an Animal Model of Huntington's Disease
M. B. Bogdanov (1998)
Mitochondrial Oxygen Radical Generation and Leak: Sites of Production in States 4 and 3, Organ Specificity, and Relation to Aging and Longevity
G. Barja (1999)
Three Classes of Inhibitors Share a Common Binding Domain in Mitochondrial Complex I (NADH:Ubiquinone Oxidoreductase)*
J. Okun (1999)
Generation of superoxide in cardiomyocytes during ischemia before reperfusion.
L. Becker (1999)
Free-living nematodes Caenorhabditis elegans possess in their mitochondria an additional rhodoquinone, an essential component of the eukaryotic fumarate reductase system.
S. Takamiya (1999)
Mitochondrial permeability transition and swelling can occur reversibly without inducing cell death in intact human cells.
Tetsuhiro Minamikawa (1999)
Effect of aging.
W. Kohrt (2000)
Mitochondrial function in heart muscle from patients with idiopathic dilated cardiomyopathy.
D. Jarreta (2000)
Mice with a Partial Deficiency of Manganese Superoxide Dismutase Show Increased Vulnerability to the Mitochondrial Toxins Malonate, 3-Nitropropionic Acid, and MPTP
O. Andreassen (2001)
DeltaPsi(m)-Dependent and -independent production of reactive oxygen species by rat brain mitochondria.
T. Votyakova (2001)
Mitochondrial toxin 3-nitropropionic acid induces cardiac and neurotoxicity differentially in mice.
K. Gabrielson (2001)
ΔΨm‐Dependent and ‐independent production of reactive oxygen species by rat brain mitochondria
T. Votyakova (2001)
Human NADH:Ubiquinone Oxidoreductase
J. Smeitink (2001)
Oxidative Stress and Neuronal DNA Fragmentation Mediate Age-Dependent Vulnerability to the Mitochondrial Toxin, 3-Nitropropionic Acid, in the Mouse Striatum
G. Kim (2001)
Altered mitochondrial function and overgeneration of reactive oxygen species precede the induction of apoptosis by 1‐O‐octadecyl‐2‐methyl‐rαc‐grycero‐3‐phosphocholine in p53‐defective hepatocytes
ANGELICA S. VRABLIC (2001)
Estradiol protects against ATP depletion, mitochondrial membrane potential decline and the generation of reactive oxygen species induced by 3‐nitroproprionic acid in SK‐N‐SH human neuroblastoma cells
J. Wang (2001)
The site of production of superoxide radical in mitochondrial Complex I is not a bound ubisemiquinone but presumably iron–sulfur cluster N2
Maria Luisa Genova (2001)
“CHEMICAL PRECONDITIONING” BY 3-NITROPROPIONATE REDUCES ISCHEMIA-REPERFUSION INJURY IN CARDIAC-ARRESTED RAT LUNGS1
T. Hirata (2001)
H. Wada (2001)
In vitro quantitation of biological superoxide and hydrogen peroxide generation.
K. Messner (2002)
Estrogen blocks 3-nitropropionic acid-induced [Ca2+]i increase and cell damage in cultured rat cerebral endothelial cells
M. Mogami (2002)
The effect of aging on p38 signaling pathway activity in the mouse liver and in response to ROS generated by 3-nitropropionic acid
C. Hsieh (2002)
The effect of aging on p 38 signaling pathway acti v ity in the mouse li v er and in response to ROS generated by 3-nitropropionic acid
C. Hsieh (2002)
Enhanced mtDNA repair capacity protects pulmonary artery endothelial cells from oxidant-mediated death.
Allison W. Dobson (2002)
In vitro quantitation
Estrogen blocks 3-nitropropionic acid-induced[Ca[2+]]i increase and cell damage in cultured rat cerebral endothelial cells
最上 美保子 (2003)
Age-associated changes in SAPK/JNK and p38 MAPK signaling in response to the generation of ROS by 3-nitropropionic acid
C. Hsieh (2003)
Age-associated changes in SAPK / JNK and p 38 MAPK signaling in response to the generation of ROS by 3-nitropropionic acid
C. Hsieh (2003)
Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide.
H. Zhao (2003)
Production of Reactive Oxygen Species by Mitochondria
Qun Chen (2003)
Essential role of complex II of the respiratory chain in hypoxia-induced ROS generation in the pulmonary vasculature.
R. Paddenberg (2003)
Microglial activation and cell death induced by the mitochondrial toxin 3-nitropropionic acid: in vitro and in vivo studies
J. Ryu (2003)
Aeromonas hydrophila Cytotoxic Enterotoxin Activates Mitogen-activated Protein Kinases and Induces Apoptosis in Murine Macrophages and Human Intestinal Epithelial Cells*
C. Galindo (2004)
Inhibitors of the Quinone-binding Site Allow Rapid Superoxide Production from Mitochondrial NADH:Ubiquinone Oxidoreductase (Complex I)*
A. Lambert (2004)
Protective effect of melatonin on 3‐nitropropionic acid‐induced oxidative stress in synaptosomes in an animal model of Huntington's disease
I. Túnez (2004)
Proteomic analysis of oxidatively modified proteins induced by the mitochondrial toxin 3-nitropropionic acid in human astrocytes expressing the HIV protein tat.
C. Pocernich (2005)
Oxidative stress in transgenic mice with oligodendroglial alpha-synuclein overexpression replicates the characteristic neuropathology of multiple system atrophy.
N. Stefanova (2005)
Crystal Structure of Mitochondrial Respiratory Membrane Protein Complex II
F. Sun (2005)
3-nitropropionic acid-induced hydrogen peroxide, mitochondrial DNA damage, and cell death are attenuated by Bcl-2 overexpression in PC12 cells.
B. Mandavilli (2005)
3-Nitropropionic Acid Is a Suicide Inhibitor of Mitochondrial Respiration That, upon Oxidation by Complex II, Forms a Covalent Adduct with a Catalytic Base Arginine in the Active Site of the Enzyme*
L. Huang (2006)
This paper is referenced by
The Pivotal Neuroinflammatory, Therapeutic and Neuroprotective Role of Alpha-Mangostin
S. D. Richard (2017)
3-NP-induced Huntington's-like disease impairs Nrf2 activation without loss of cardiac function in aged rats
A. Silva-Palacios (2017)
2-1-2014 Repair of Oxidative DNA Damage and Cancer : Recent Progress in DNA Base Excision Repair
Timothy L. Scott (2017)
Selective downregulation of mitochondrial electron transport chain activity and increased oxidative stress in human atrial fibrillation.
L. Emelyanova (2016)
Neuroprotective effect of caffeic acid phenethyl ester in 3-nitropropionic acid-induced striatal neurotoxicity
Jia Bak (2016)
Mitochondrial succinate dehydrogenase is involved in stimulus-secretion coupling and endogenous ROS formation in murine beta cells
Armin Edalat (2015)
Oxidative modification enhances the immunostimulatory effects of extracellular mitochondrial DNA on plasmacytoid dendritic cells.
K. Pázmándi (2014)
Obligatory Role of Intraluminal O2− in Acute Endothelin-1 and Angiotensin II Signaling to Mediate Endothelial Dysfunction and MAPK Activation in Guinea-Pig Hearts
Emilia Wojtera (2014)
Repair of oxidative DNA damage and cancer: recent progress in DNA base excision repair.
Timothy L. Scott (2014)
3-Nitropropionic Acid Induces Ovarian Oxidative Stress and Impairs Follicle in Mouse
J. Zhang (2014)
Estudo do Efeito Neuroprotetor do Eugenol em Modelo Experimental da Doença de Huntington
Rafaella Farias da Nóbrega (2013)
Mitochondria and Cytoprotection
C. Brenner (2012)
Mitochondria: Redox Metabolism and Dysfunction
Jia Kang (2012)
Collapsin response mediator protein‐2 phosphorylation promotes the reversible retraction of oligodendrocyte processes in response to non‐lethal oxidative stress
Á. Fernández-Gamba (2012)
Biochemical identification of a hydroperoxide derivative of the free 8-oxo-7,8-dihydroguanine base.
G. Hajas (2012)
Warburg Effect’s Manifestation in Aggressive Pheochromocytomas and Paragangliomas: Insights from a Mouse Cell Model Applied to Human Tumor Tissue
Stephanie M J Fliedner (2012)
Models of Mitochondrial Oxidative Stress
E. Cadenas (2011)
Lactoferrin decreases LPS-induced mitochondrial dysfunction in cultured cells and in animal endotoxemia model
M. Kruzel (2010)
Hypoxia sensing in the fetal chicken femoral artery is mediated by the mitochondrial electron transport chain.
B. Zoer (2010)
Microbial biodegradation potential of hydrocarbons evaluated by colorimetric technique: a case study
E. D. Bidoia (2010)
ATP Depletion Via Mitochondrial F1F0 Complex by Lethal Factor is an Early Event in B. Anthracis-Induced Sudden Cell Death
Mitchell W Woodberry (2009)
ROS scavenging capacity and neuroprotective effect of alpha-mangostin against 3-nitropropionic acid in cerebellar granule neurons.
J. Pedraza-Chaverri (2009)
Mitochondria as targets for cancer therapy.
S. Ralph (2009)
Effects of mitochondrial poisons on glutathione redox potential and carotid body chemoreceptor activity
A. Gomez-Niño (2009)
Opening of the mitoKATP channel and decoupling of mitochondrial complex II and III contribute to the suppression of myocardial reperfusion hyperoxygenation
B. Liu (2009)
Heme oxygenase-1 induction prevents neuronal damage triggered during mitochondrial inhibition: role of CO and bilirubin.
M. Orozco-Ibarra (2009)
Neurotoxins and neurotoxicity mechanisms. an overview
J. Segura-Aguilar (2009)
Ragweed pollen-mediated IgE-independent release of biogenic amines from mast cells via induction of mitochondrial dysfunction.
G. Chodaczek (2009)
Mitochondrial Dysfunction Increases Allergic Airway Inflammation1
L. Aguilera-Aguirre (2009)
Mitochondrial DNA damage is a hallmark of chemically induced and the R6/2 transgenic model of Huntington's disease.
Karina Acevedo-Torres (2009)
Reactive oxygen species generation at the plasma membrane for antibody control.
F. Crane (2008)
Endogenous dopamine enhances the neurotoxicity of 3-nitropropionic acid in the striatum through the increase of mitochondrial respiratory inhibition and free radicals production.
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