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Hydrogen Peroxide Induces Apoptosis In HeLa Cells Through Mitochondrial Pathway.

Mayank Singh, H. Sharma, Neeta Singh
Published 2007 · Medicine, Biology

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Cervical cancer is the most common cancer amongst females in India and is associated with high risk HPVs, reactive oxygen species (ROS), and excessive inflammation in most cases. ROS in turn affects the expression of pro- and anti-apoptotic proteins. The objective of the present study was to elucidate the effect of hydrogen peroxide (H(2)O(2)) on apoptotic signaling molecules in vitro. HeLa cell line expresses the Human papilloma virus - 18, E6 oncoprotein which causes the ubiquitin mediated degradation of p53 protein and is thus p53 deficient. p53 is known to act as a cellular stress sensor and triggers apoptosis. p73, a member of the p53 family also induces apoptosis in response to DNA damaging agents but unlike p53, it is infrequently mutated in human tumors. We demonstrate here, that in HeLa cells, apoptosis is triggered by H(2)O(2) via the mitochondrial pathway involving upregulation of p73, and its downstream target Bax. This was accompanied by upregulation of ERK, JNK, c-Myc, Hsp-70 and down regulation of anti-apoptotic Bcl-XL, release of cytochrome c from mitochondria and activation of caspases-9 and -3.
This paper references
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding
M. M. Bradford (1976)
Association of human papillomavirus types 16 and 18 E6 proteins with p53.
B. Werness (1990)
Association of human papilloma virus type 16 and 18 E6 protein with p53
B. A. Werness (1990)
The MYC protein activates transcription of the alpha‐prothymosin gene.
M. Eilers (1991)
Reactive Oxygen Species and the Central Nervous System
B. Halliwell (1992)
Cell death.
L. M. Schwartz (1995)
Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis
Zhengui Xia (1995)
Opposing effect of ERK and JNK-p38 MAPK on apoptosis
Z. Xia (1995)
Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c
X. Liu (1996)
Induction of apoptotic programme in cell free extracts ; requirement for dATP and cytochrome c
X. Liu (1996)
Monoallelically Expressed Gene Related to p53 at 1p36, a Region Frequently Deleted in Neuroblastoma and Other Human Cancers
M. Kaghad (1997)
Metal ion-dependent hydrogen peroxide-induced DNA damage is more sequence specific than metal specific.
H. Rodriguez (1997)
Double identity for proteins of the Bcl-2 family
John Calvin Reed (1997)
Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade
Peng Li (1997)
Transactivation-defective c-MycS retains the ability to regulate proliferation and apoptosis.
Q. Xiao (1998)
The Bcl-2 protein family: arbiters of cell survival.
J. Adams (1998)
A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD
M. Enari (1998)
Mammalian caspases: structure, activation, substrates, and functions during apoptosis.
W. Earnshaw (1999)
Apoptotic cytosol facilitates Bax translocation to mitochondria that involves cytosolic factor regulated by Bcl-2.
M. Nomura (1999)
p53 family genes: structural comparison, expression and mutation
S. Ikawa (1999)
Apoptosis signaling.
A. Strasser (2000)
Smac, a Mitochondrial Protein that Promotes Cytochrome c–Dependent Caspase Activation by Eliminating IAP Inhibition
C. Du (2000)
Oncogenic mutations of the p53 tumor suppressor: the demons of the guardian of the genome.
A. Sigal (2000)
Structural basis of IAP recognition by Smac/DIABLO
G. Wu (2000)
Bcl-2 inhibits Bax translocation from cytosol to mitochondria during drug-induced apoptosis of human tumor cells
K. Murphy (2000)
Apoptotic DNA fragmentation.
S. Nagata (2000)
Role of the heat shock response and molecular chaperones in oncogenesis and cell death.
C. Jolly (2000)
Anti-apoptotic effect of quercetin: intervention in the JNK- and ERK-mediated apoptotic pathways.
Y. Ishikawa (2000)
Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death
N. Joza (2001)
Insights into the molecular mechanism of apoptosis induced by TNF-alpha in mouse epidermal JB6-derived RT-101 cells.
N. Singh (2002)
p53 polymorphism influences response in cancer chemotherapy via modulation of p73-dependent apoptosis.
D. Bergamaschi (2003)
Functional regulation of p73 and p63: development and cancer.
G. Melino (2003)
The selection between apoptosis and necrosis is differentially regulated in hydrogen peroxide-treated and glutathione-depleted human promonocytic cells
A. Troyano (2003)
Activation of ERK1/2, JNK and PKB by hydrogen peroxide in human SH-SY5Y neuroblastoma cells: role of ERK1/2 in H2O2-induced cell death.
James Ruffels (2004)
p73 Induces Apoptosis via PUMA Transactivation and Bax Mitochondrial Translocation*
G. Melino (2004)
JNK activation is a mediator of arsenic trioxide-induced apoptosis in acute promyelocytic leukemia cells.
K. Davison (2004)
Hsp70 chaperones: Cellular functions and molecular mechanism
M. Mayer (2005)
Curcumin enhances Vinorelbine mediated apoptosis in NSCLC cells by the mitochondrial pathway.
S. Sen (2005)
p73, a sophisticated p53 family member in the cancer world
T. Ozaki (2005)
Molecular pathways in the chemosensitization of cisplatin by quercetin in human head and neck cancer
H. Sharma (2005)
Role of mitochondria in quercetin-enhanced chemotherapeutic response in human non-small cell lung carcinoma H-520 cells.
Meenakshi Kuhar (2006)

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