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Hydrogen Peroxide Induces Apoptosis In HeLa Cells Through Mitochondrial Pathway.

Mayank Singh, H. Sharma, Neeta Singh
Published 2007 · Medicine, Biology

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Cervical cancer is the most common cancer amongst females in India and is associated with high risk HPVs, reactive oxygen species (ROS), and excessive inflammation in most cases. ROS in turn affects the expression of pro- and anti-apoptotic proteins. The objective of the present study was to elucidate the effect of hydrogen peroxide (H(2)O(2)) on apoptotic signaling molecules in vitro. HeLa cell line expresses the Human papilloma virus - 18, E6 oncoprotein which causes the ubiquitin mediated degradation of p53 protein and is thus p53 deficient. p53 is known to act as a cellular stress sensor and triggers apoptosis. p73, a member of the p53 family also induces apoptosis in response to DNA damaging agents but unlike p53, it is infrequently mutated in human tumors. We demonstrate here, that in HeLa cells, apoptosis is triggered by H(2)O(2) via the mitochondrial pathway involving upregulation of p73, and its downstream target Bax. This was accompanied by upregulation of ERK, JNK, c-Myc, Hsp-70 and down regulation of anti-apoptotic Bcl-XL, release of cytochrome c from mitochondria and activation of caspases-9 and -3.
This paper references
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding
M. M. Bradford (1976)
10.1126/SCIENCE.2157286
Association of human papillomavirus types 16 and 18 E6 proteins with p53.
B. Werness (1990)
Association of human papilloma virus type 16 and 18 E6 protein with p53
B. A. Werness (1990)
10.1002/j.1460-2075.1991.tb07929.x
The MYC protein activates transcription of the alpha‐prothymosin gene.
M. Eilers (1991)
10.1111/j.1471-4159.1992.tb10990.x
Reactive Oxygen Species and the Central Nervous System
B. Halliwell (1992)
10.1002/9780813810379.ch2
Cell death.
L. M. Schwartz (1995)
10.1126/science.270.5240.1326
Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis
Zhengui Xia (1995)
Opposing effect of ERK and JNK-p38 MAPK on apoptosis
Z. Xia (1995)
10.1016/S0092-8674(00)80085-9
Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c
X. Liu (1996)
Induction of apoptotic programme in cell free extracts ; requirement for dATP and cytochrome c
X. Liu (1996)
10.1016/S0092-8674(00)80540-1
Monoallelically Expressed Gene Related to p53 at 1p36, a Region Frequently Deleted in Neuroblastoma and Other Human Cancers
M. Kaghad (1997)
Metal ion-dependent hydrogen peroxide-induced DNA damage is more sequence specific than metal specific.
H. Rodriguez (1997)
10.1038/42867
Double identity for proteins of the Bcl-2 family
John Calvin Reed (1997)
10.1016/S0092-8674(00)80434-1
Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade
Peng Li (1997)
10.1101/GAD.12.24.3803
Transactivation-defective c-MycS retains the ability to regulate proliferation and apoptosis.
Q. Xiao (1998)
10.1126/SCIENCE.281.5381.1322
The Bcl-2 protein family: arbiters of cell survival.
J. Adams (1998)
10.1038/34112
A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD
M. Enari (1998)
10.1146/ANNUREV.BIOCHEM.68.1.383
Mammalian caspases: structure, activation, substrates, and functions during apoptosis.
W. Earnshaw (1999)
Apoptotic cytosol facilitates Bax translocation to mitochondria that involves cytosolic factor regulated by Bcl-2.
M. Nomura (1999)
10.1038/sj.cdd.4400631
p53 family genes: structural comparison, expression and mutation
S. Ikawa (1999)
10.1146/annurev.biochem.69.1.217
Apoptosis signaling.
A. Strasser (2000)
10.1016/S0092-8674(00)00008-8
Smac, a Mitochondrial Protein that Promotes Cytochrome c–Dependent Caspase Activation by Eliminating IAP Inhibition
C. Du (2000)
Oncogenic mutations of the p53 tumor suppressor: the demons of the guardian of the genome.
A. Sigal (2000)
10.1038/35050012
Structural basis of IAP recognition by Smac/DIABLO
G. Wu (2000)
10.1038/sj.cdd.4400597
Bcl-2 inhibits Bax translocation from cytosol to mitochondria during drug-induced apoptosis of human tumor cells
K. Murphy (2000)
10.1006/EXCR.2000.4834
Apoptotic DNA fragmentation.
S. Nagata (2000)
10.1093/JNCI/92.19.1564
Role of the heat shock response and molecular chaperones in oncogenesis and cell death.
C. Jolly (2000)
10.1046/J.1523-1755.2000.00265.X
Anti-apoptotic effect of quercetin: intervention in the JNK- and ERK-mediated apoptotic pathways.
Y. Ishikawa (2000)
10.1038/35069004
Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death
N. Joza (2001)
10.1016/S0006-291X(02)00627-7
Insights into the molecular mechanism of apoptosis induced by TNF-alpha in mouse epidermal JB6-derived RT-101 cells.
N. Singh (2002)
10.1016/S1535-6108(03)00079-5
p53 polymorphism influences response in cancer chemotherapy via modulation of p73-dependent apoptosis.
D. Bergamaschi (2003)
10.1016/J.TIBS.2003.10.004
Functional regulation of p73 and p63: development and cancer.
G. Melino (2003)
10.1038/sj.cdd.4401249
The selection between apoptosis and necrosis is differentially regulated in hydrogen peroxide-treated and glutathione-depleted human promonocytic cells
A. Troyano (2003)
10.1016/J.EJPHAR.2003.10.032
Activation of ERK1/2, JNK and PKB by hydrogen peroxide in human SH-SY5Y neuroblastoma cells: role of ERK1/2 in H2O2-induced cell death.
James Ruffels (2004)
10.1074/jbc.M307469200
p73 Induces Apoptosis via PUMA Transactivation and Bax Mitochondrial Translocation*
G. Melino (2004)
10.1182/BLOOD-2003-05-1412
JNK activation is a mediator of arsenic trioxide-induced apoptosis in acute promyelocytic leukemia cells.
K. Davison (2004)
10.1007/s00018-004-4464-6
Hsp70 chaperones: Cellular functions and molecular mechanism
M. Mayer (2005)
10.1016/J.BBRC.2005.04.044
Curcumin enhances Vinorelbine mediated apoptosis in NSCLC cells by the mitochondrial pathway.
S. Sen (2005)
10.1111/j.1349-7006.2005.00116.x
p73, a sophisticated p53 family member in the cancer world
T. Ozaki (2005)
10.4161/cbt.4.9.1908
Molecular pathways in the chemosensitization of cisplatin by quercetin in human head and neck cancer
H. Sharma (2005)
Role of mitochondria in quercetin-enhanced chemotherapeutic response in human non-small cell lung carcinoma H-520 cells.
Meenakshi Kuhar (2006)



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10.1007/s11033-021-06695-1
Neoxanthin prevents H2O2-induced cytotoxicity in HepG2 cells by activating endogenous antioxidant signals and suppressing apoptosis signals.
D. Udayawara Rudresh (2021)
10.1111/cas.14890
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M. Nakashima (2021)
10.3389/fcell.2021.666484
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Li Zhang (2021)
10.1016/j.freeradbiomed.2021.11.006
Dissecting in vivo and in vitro redox responses using chemogenetics.
Markus Waldeck-Weiermair (2021)
10.1007/978-981-15-4501-6_48-1
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Saranya NavaneethaKrishnan (2021)
10.1152/ajpheart.00956.2020
Apoptosis in resistance arteries induced by hydrogen peroxide: Greater resilience of endothelium vs. smooth muscle.
R. L. Shaw (2021)
10.1007/s00294-021-01204-0
Contribution of SOS genes to H2O2-induced apoptosis-like death in Escherichia coli.
Heesu Kim (2021)
10.1155/2021/1341604
Interplay between Mitochondrial Metabolism and Cellular Redox State Dictates Cancer Cell Survival
Brittney Joy-Anne Foo (2021)
10.1016/J.AQUACULTURE.2021.737040
Pomelo polysaccharide extract inhibits oxidative stress, inflammation, and mitochondrial apoptosis of Epinephelus coioides
Huifan Liu (2021)
10.1016/j.jconrel.2021.01.034
Oxygen-release microspheres capable of releasing oxygen in response to environmental oxygen level to improve stem cell survival and tissue regeneration in ischemic hindlimbs.
Ya Guan (2021)
10.3390/molecules26051374
Cytotoxic Mechanism of Sphaerodactylomelol, an Uncommon Bromoditerpene Isolated from Sphaerococcus coronopifolius
Celso Alves (2021)
10.1016/j.neuro.2021.05.014
Formononetin attenuates H2O2-induced cell death through decreasing ROS level by PI3K/Akt-Nrf2-activated antioxidant gene expression and suppressing MAPK-regulated apoptosis in neuronal SH-SY5Y cells.
Mayuko Sugimoto (2021)
10.1111/1750-3841.15419
Lycopene protects neuroblastoma cells against oxidative damage via depression of ER stress.
Shanshan Ou (2020)
10.1155/2020/2468031
Wenxin Keli Regulates Mitochondrial Oxidative Stress and Homeostasis and Improves Atrial Remodeling in Diabetic Rats
(2020)
10.1128/mBio.01704-20
A Genome-Wide CRISPR/Cas9 Screen Reveals that Riboflavin Regulates Hydrogen Peroxide Entry into HAP1 Cells
Tamutenda Chidawanyika (2020)
10.1042/BST20200110
Cytotoxicity of snake venom enzymatic toxins: phospholipase A2 and l-amino acid oxidase
J. Hiu (2020)
10.1091/mbc.E20-01-0063
Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking
Stephen C Ireland (2020)
10.3390/medicina56120663
A New Insight on the Radioprotective Potential of Epsilon-Aminocaproic Acid
T. Saliev (2020)
10.1074/jbc.RA119.011870
PCBP1 and PCBP2 both bind heavily oxidized RNA but cause opposing outcomes, suppressing or increasing apoptosis under oxidative conditions
T. Ishii (2020)
10.5772/INTECHOPEN.93568
Role of Subcellular ROS in Providing Resilience to Vascular Endothelium
Sarah R. Aldosari (2020)
10.1016/j.freeradbiomed.2020.03.019
Methionine sulfoxide reductase A (MsrA) modulates cells and protects against Mycoplasma genitalium induced cytotoxicity.
K. Das (2020)
10.1017/S0031182020000438
The effect of iron on Trichomonas vaginalis TvCP2: a cysteine proteinase found in vaginal secretions of trichomoniasis patients
Luis Alberto Rivera-Rivas (2020)
10.31557/APJCP.2020.21.12.3697
Myricetin Apoptotic Effects on T47D Breast Cancer Cells is a P53-Independent Approach
M. Soleimani (2020)
10.1074/jbc.REV120.011149
The many lives of Myc in the pancreatic β-cell
Carolina Rosselot (2020)
10.1021/acsomega.9b04208
Design and Development of an HBT-Based Ratiometric Fluorescent Probe to Monitor Stress-Induced Premature Senescence
J. N. Makau (2020)
10.1016/j.ejbt.2020.07.006
The protective ability and cellular mechanism of Koelreuteria henryi Dummer flower extract against hydrogen peroxide-induced cellular oxidative damage
Wen-Che Tsai (2020)
10.1016/j.aquatox.2020.105657
Chronic exposure of hydrogen peroxide alters redox state, apoptosis and endoplasmic reticulum stress in common carp (Cyprinus carpio).
Rui Jia (2020)
10.1016/j.cryobiol.2020.10.001
The efficacy of ice recrystallization inhibitors in rat lung cryopreservation using a low cost technique for ex vivo subnormothermic lung perfusion.
L. Lautner (2020)
10.1002/smll.202003496
Oxygen-Deficient Bimetallic Oxide FeWOX Nanosheets as Peroxidase-Like Nanozyme for Sensing Cancer via Photoacoustic Imaging.
Fei Gong (2020)
10.1101/2020.09.08.287680
Regeneration of lung epithelial cells by Fullerene C60 nanoformulation: A possible treatment strategy for acute respiratory distress syndrome (ARDS)
Nabodita Sinha (2020)
Cytoprotective mechanisms, palatogenesis, and wound repair
N. Cremers (2019)
10.3892/mmr.2019.10727
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Wenbo Tang (2019)
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