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Pectin Methyl Esterases And Pectins In Normal And Hyperhydric Shoots Of Carnation Cultured In Vitro.

Shady Saher, A. Piqueras, E. Hellín, E. Olmos
Published 2005 · Biology, Medicine

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Control and hyperhydric micropropagated plantlets from three carnation cultivars have been used to study their pectin composition and the activity of pectin methyl esterases (PMEs; EC 3.1.1.11). Pectins are a highly heterogeneous group of polymers that contribute to cell adhesion, cell wall architecture, and cell wall mechanical strength. Pectins control cell wall porosity and cell wall ionic status and are implicated in intercellular space development. The degree of esterification of pectins is controlled by the activity of cell wall PMEs; their different actions can affect the properties of the cell wall, which have been considered important with respect to controlling the development of hyperhydricity. The total pectins of hyperhydric leaves of the three varieties were significantly reduced in comparison with controls. The pectate fraction was significantly increased in hyperhydric leaves of all varieties while soluble pectins and protopectins were significantly lower. The PME activity of hyperhydric leaves was higher (4-10 times) compared to controls of the three varieties. Isoelectric focusing of PME isozymes revealed the presence of three isoforms; neutral PME activity was the major isozyme in control and hyperhydric leaves of the three varieties, whilst a decrease in the activity of the acidic isoforms was observed in hyperhydric leaves. The different PME activities could regulate some of the structural changes related to hyperhydricity in micropropagated carnation plants.
This paper references
10.1111/J.1365-2621.1964.TB01690.X
Seasonal Changes Occurring in the Pectinesterase Activity and Pectic Constituents of the Component Parts of Citrus Fruits II. Pineapple Orangesa
A. Rouse (1962)
10.1016/S1360-1385(01)02045-3
Pectin methylesterases: cell wall enzymes with important roles in plant physiology.
F. Micheli (2001)
10.3109/10520299809140504
Cytological comparison of leaves and stems of Prunus avium L. shoots cultured on a solid medium with agar or gelrite.
T. Franck (1998)
10.1007/s004250050104
Pectin methylesterases from poplar cambium and inner bark: localization, properties and seasonal changes
N. Guglielmino (1997)
10.1046/J.1365-3040.2003.01034.X
Intercellular adhesion and cell separation in plants
M. Jarvis (2003)
10.1080/08905439809549953
A study of the cell wall mechanical properties in unhyperhydrated shoots of oregano (origanum vulgare) inoculated with pseudomonas sp. by load deformation analysis
Avadhani S. Komali (1998)
10.1016/S0304-4238(98)00096-X
Ultrastructural differences of hyperhydric and normal leaves from regenerated carnation plants
E. Olmos (1998)
10.1007/BF00029283
Water in aerenchyma spaces in roots. A fast diffusion path for solutes
C. V. D. Weele (2004)
10.1111/J.1399-3054.1984.TB06102.X
Physiological and biochemical events leading to vitrification of plants cultured in vitro
C. Kevers (1984)
10.1016/S0168-9452(97)00214-8
The subcellular localization of peroxidase and the implication of oxidative stress in hyperhydrated leaves of regenerated carnation plants
E. Olmos (1997)
10.1104/PP.124.1.231
An increase in pectin methyl esterase activity accompanies dormancy breakage and germination of yellow cedar seeds.
C. Ren (2000)
10.1016/S0008-6215(00)00070-7
Different action patterns for apple pectin methylesterase at pH 7.0 and 4.5.
J. Denès (2000)
10.1016/S0921-0423(96)80253-X
Methyl-esterification, de-esterification and gelation of pectins in the primary cell wall
R. Goldberg (1996)
10.1007/BF00034307
Reconsideration of the term ‘vitrification’ as used in micropropagation
P. Debergh (2004)
10.1023/A:1010650131732
Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation
J. Majada (2004)
Pectins in normal and vitreous apple microplants cultured in liquid medium
F. Marga (1995)
10.1007/BF00035020
Vitrification of carnation in vitro: Changes in cell wall mechanical properties, cellulose and lignin content
C. Kevers (2004)
10.1046/J.1365-3040.1998.00363.X
Intercellular separation forces generated by intracellular pressure
M. Jarvis (1998)
10.1016/S0981-9428(00)00169-8
The three-dimensional structures of the pectic polysaccharides
S. Pérez (2000)
10.1034/J.1399-3054.2000.100408.X
Pectin methylesterase modulates aluminium sensitivity in Zea mays and Solanum tuberosum
N. Schmohl (2000)
10.1111/J.0031-9317.2004.0219.X
Hyperhydricity in micropropagated carnation shoots: the role of oxidative stress.
Shady Saher (2004)
10.1023/B:TICU.0000016825.18930.e4
Hyperhydricity of Micropropagated Shoots: A Typically Stress-induced Change of Physiological State
C. Kevers (2004)
10.1016/J.PLAPHY.2004.05.003
Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response.
T. Franck (2004)



This paper is referenced by
Towards automating micropropagation: from cells to shoots to plants in one step
Liwen Fei (2015)
10.15835/NBHA4016566
Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro
C. Yang (2012)
10.1016/J.ENVEXPBOT.2007.08.004
Sub-cellular location of H2O2, peroxidases and pectin epitopes in control and hyperhydric shoots of carnation
N. Fernández-García (2008)
10.1016/J.PLAPHY.2005.05.006
Reducing properties, energy efficiency and carbohydrate metabolism in hyperhydric and normal carnation shoots cultured in vitro: a hypoxia stress?
Shady Saher (2005)
10.3923/AJBKR.2017.1.23
Biochemical and Molecular Characterization of Cell Wall Degrading Enzyme, Pectin Methylesterase Versus Banana Ripening: An Overview
C. Verma (2016)
10.1007/s11240-018-1506-6
Regulation of cell wall development in Brachypodium distachyon in vitro as affected by cytokinin and gas exchange
T. C. Mamedes-Rodrigues (2018)
10.1007/s10535-008-0124-2
The differences in cell wall composition in leaves and regenerating protoplasts of Beta vulgaris and Nicotiana tabacum
E. Wiśniewska (2008)
Cell Wall Metabolism of Two Varieties of Ber (Ziziphus mauritiana Lam.) Fruit During Ripening
P. Yadav (2012)
Characterization of two chloroplast envelope membrane proteins
Nannan Li (2012)
The biochemistry and molecular biology of intercellular adhesion in plant tissue culture
L. Qouta (2008)
In Vitro Liquid Culture Systems of Scutellaria Species
A. Tascan (2007)
Cytokinins induce hyperhydricity in leaves of in vitro grown Arabidopsis thaliana
Ziqi Zeng Reg (2016)
10.1201/9781439854082-13
Metal Toxicity, Oxidative Stress and Antioxidative Defense System in Plants
Nieves Fernández-García (2010)
Proteomic analysis of b-aminobutyric acid priming and abscisic acid – induction of drought resistance in crabapple (Malus pumila): effect on general metabolism, the phenylpropanoid pathway and cell wall enzymespce_2025 1612..1631
D. Macarisin (2009)
10.1111/J.1365-3040.2009.02025.X
Proteomic analysis of β‐aminobutyric acid priming and abscisic acid – induction of drought resistance in crabapple (Malus pumila): effect on general metabolism, the phenylpropanoid pathway and cell wall enzymes
D. Macarisin (2009)
10.1002/9781118324097.CH3
Enzymes in Quality and Processing of Tropical and Subtropical Fruits
A. Liavoga (2012)
10.1016/J.TPLANTS.2007.04.001
New insights into pectin methylesterase structure and function.
J. Pelloux (2007)
10.1007/BF03323436
Mago nashi Interacts with a Taiwania (Taiwania cryptomerioides) Pectin Methylesterase-like Protein
Y. R. Chen (2013)
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