Online citations, reference lists, and bibliographies.
← Back to Search

Antioxidant And Chromium Reductase Assisted Chromium (VI) Reduction And Cr (III) Immobilization By The Rhizospheric Bacillus Helps In The Remediation Of Cr (VI) And Growth Promotion Of Soybean Crop

P. A. Wani, S. Wahid, Ruchi Singh, A. M. Kehinde
Published 2018 · Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
Abstract Cr (VI) is used in various industries and its improper treatment lead to the contamination of environment with hexavalent chromium. Microbes convert toxic Cr (VI) to less soluble Cr (III), thus can be used for detoxification of Cr (VI) from contaminated environment. On the basis of above facts, present study was designed to see the effect of chromium reductases and antioxidants produced by Bacillus subtilus MAI3 for chromium (VI) detoxification and augmenting soybean growth. Optimum pH for the reduction of Cr (VI) by Bacillus species MAI3 was found to be 7. Maximum chromium (VI) was significantly reduced at 50, 100 µg/ml of chromium (VI), 25 and 35 °C of temperature but showed less reduction at 45 °C. The presence of reduced Cr (III) in solution (supernatant) and pellet was found to be 30 ± 1.0 and 57 ± 2.5 µg/ml after reduction of Cr (VI) by Bacillus strain MAI3. This indicates that a majority of the reduced Cr (III) is immobilized by the Bacillus strain MAI3. Chromium reductase found in cell free extract reduced almost all chromium (VI) compared to cell debris which has not shown any chromium (VI) reduction. As the concentration of metal increased, MDA also increased. There was an increase in the antioxidant levels upon exposure to chromium (VI). Bacillus strain MAI3 inoculated to the soybean confirmed the role of antioxidants and reductases for augmenting growth and photosynthetic pigments of soybean under chromium (VI). Bacillus strain MAI3 reduced almost all Cr (VI) into Cr (III) in soil, most of which was immobilized by the plant. Thus both antioxidants and Cr (III) immobilization were reason for increased growth of soybean under metal stress.
This paper references
10.1007/S11274-009-0047-X
Removal of toxic chromate using free and immobilized Cr(VI)-reducing bacterial cells of Intrasporangium sp. Q5-1
J. Yang (2009)
10.1371/journal.pone.0016634
Surface-Enhanced Raman Imaging of Intracellular Bioreduction of Chromate in Shewanella oneidensis
S. Ravindranath (2011)
10.1016/j.chemosphere.2013.08.080
Investigation on mechanism of Cr(VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil.
S. Das (2014)
10.4236/JEP.2011.21008
Studies on Chromate Removal by Chromium-Resistant Bacillus sp. Isolated from Tannery Effluent
M. K. Chaturvedi (2011)
10.1007/s00128-013-1002-y
Nickel Detoxification and Plant Growth Promotion by Multi Metal Resistant Plant Growth Promoting Rhizobium Species RL9
P. A. Wani (2013)
10.1016/j.biortech.2007.12.046
Evaluation of in vitro Cr(VI) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr(VI) polluted industrial landfill.
Chirayu Desai (2008)
10.1007/s10529-005-5526-z
Reduction of Cr(VI) by a Bacillus sp.
R. Elangovan (2005)
10.1016/J.BIORTECH.2005.12.025
Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals.
S. Sultan (2007)
10.1007/s00284-002-3889-0
Toxicity of Hexavalent Chromium and Its Reduction by Bacteria Isolated from Soil Contaminated with Tannery Waste
M. Megharaj (2003)
Purification and partial characterization of a chromate reductase from Bacillus.
J. Campos-García (1997)
10.1016/j.jhazmat.2009.09.134
Chromium (VI) biotransformation by beta- and gamma-Proteobacteria from natural polluted environments: a combined biological and chemical treatment for industrial wastes.
L. Garavaglia (2010)
10.1016/J.PROCBIO.2008.02.015
Hexavalent chromate reductase activity in cytosolic fractions of Pseudomonas sp. G1DM21 isolated from Cr(VI) contaminated industrial landfill
Chirayu Desai (2008)
10.1007/s11427-012-4359-y
Effects of cadmium stress on seed germination and seedling growth of Elymus dahuricus infected with the Neotyphodium endophyte
X. Zhang (2012)
Alleviation of phyto-toxic effects of chromium by inoculation of chromium (VI) reducing Pseudomonas aeruginosa Rb-1 and Ochrobactrum intermedium Rb-2.
R. Batool (2015)
10.1007/s00284-005-0048-4
Reduction of Hexavalent Chromium by Cell-Free Extract of Bacillus sphaericus AND 303 Isolated from Serpentine Soil
Arundhati Pal (2005)
Purification and characterization of NADPH-dependent Cr(VI) reductase from Escherichia coli ATCC 33456.
Woo-Chul Bae (2005)
10.6064/2012/963401
Plant Growth-Promoting Bacteria: Mechanisms and Applications
B. Glick (2012)
10.1111/J.1574-6976.2001.TB00581.X
Interactions of chromium with microorganisms and plants.
C. Cervantes (2001)
10.1016/j.biortech.2009.11.008
Proteomic changes in response to chromium(VI) toxicity in Pseudomonas aeruginosa.
N. Kılıç (2010)
10.1016/j.fct.2010.08.035
Bacillus species enhance growth parameters of chickpea (Cicer arietinum L.) in chromium stressed soils.
P. A. Wani (2010)
10.3923/JEST.2015.122.130
Bioreduction of Cr (VI) by Heavy Metal Resistant Pseudomonas Species
P. A. Wani (2015)
10.1016/S0169-5002(01)00472-X
Modulation of tumor-infiltrating lymphocyte cytolytic activity against human non-small cell lung cancer.
J. Ortegel (2002)
10.1007/0-387-21728-2_4
Chromium-microorganism interactions in soils: remediation implications.
S. P. B. Kamaludeen (2003)
10.3923/IJSS.2015.203.210
Cr (VI) Reduction by Indigenous Bacillus Species PB5 Isolated from Contaminated Soil of Abeokuta Ogun State, Nigeria
P. A. Wani (2015)
10.1007/s11356-014-3856-x
Mechanism of Cr(VI) reduction by Aspergillus niger: enzymatic characteristic, oxidative stress response, and reduction product
Y. Gu (2014)
10.3923/JM.2015.66.75
Chromium (Vi) Reduction by Streptococcus Species Isolated from the Industrial Area of Abeokuta, Ogun State, Nigeria
P. A. Wani (2015)
10.1128/AEM.70.2.873-882.2004
Chromate-Reducing Properties of Soluble Flavoproteins from Pseudomonas putida and Escherichia coli
D. Ackerley (2004)
10.1007/s002530100758
Chromate reduction and 16S rRNA identification of bacteria isolated from a Cr(VI)-contaminated site
P. Pattanapipitpaisal (2001)
10.1007/s10529-005-7188-2
Bacterial Cr(VI) Reduction Concurrently Improves Sunflower (Helianthus Annuus L.) Growth
M. Faisal (2005)
10.1007/S00128-005-0808-7
Chromate Reduction Capability of a Gram Positive Bacterium Isolated from Effluent of Dying Industry
S. Sultan (2005)
10.1016/j.jare.2016.08.007
Cellulosimicrobium funkei-like enhances the growth of Phaseolus vulgaris by modulating oxidative damage under Chromium(VI) toxicity
C. Karthik (2016)
10.1128/AEM.67.4.1517-1521.2001
Relationship of Hydrogen Bioavailability to Chromate Reduction in Aquifer Sediments
T. Marsh (2001)
10.3923/RJET.2016.144.151
Effect of Chromium (Vi) Reducing Bacillus species PZ3 on the Growth of Pea Plants in Chromium Amended Soil
P. A. Wani (2016)
Standard methods for the examination of water and wastewater : supplement to the sixteenth edition
A. E. Greenberg (1988)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
Reduction of Chromium(VI) by Locally Isolated Pseudomonas sp. C-171
Mujeeb ur Rahman (2007)
10.1104/PP.24.1.1
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
D. Arnon (1949)
10.1111/j.1751-7915.2010.00181.x
Impacts of Shewanella oneidensis c‐type cytochromes on aerobic and anaerobic respiration
H. Gao (2010)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar