Online citations, reference lists, and bibliographies.

H2O2 Metabolism During Sweet Orange (Citrus Sinensis L. Osb.) 'Hamlin' Xanthomonas Axonopodis Pv. Citri Interaction

Nuthan Kumar, Robert C. Ebel, Pamela D. Roberts
Published 2011 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
Abstract Sweet orange (Citrus sinensis L. Osb.) ‘Hamlin’ is a canker (Xanthomonas axonopodis pv. citri: Xac) susceptible citrus genotype grown commercially worldwide. Canker causes severe economic losses and restricts the marketability of crop for export. Little is known about the role of oxidative stress in canker development. In the present investigation, sweet orange ‘Hamlin’ leaves were artificially inoculated with Xac to determine the impact of Xac infection on hydrogen peroxide (H2O2) metabolism. Characteristic symptoms following artificial inoculation were water soaking of the infiltrated zone between 2 and 8 days after inoculation (dai); raised epidermis accompanying tiny yellow colored bacterial colonies at 8 dai; and yellowing and necrosis of the infected zone by 12–16 dai. In planta Xac population increased 1000 fold by 14 dai from an initial population of 7.3 × 106 cfu cm−2 (0 dai). Peak concentrations of H2O2 were observed at 24 h and between 8 and 10 dai and coincided with higher activity of total superoxide dismutase (SOD). Lower levels of H2O2 in infected leaves were maintained by Xac induced higher activities of catalase (CAT), ascorbate peroxidase (APOD), and guaiacol peroxidase (POD). It appears Xac altered H2O2 metabolism in C. sinensis L. Osb. ‘Hamlin’ to enhance survival and growth.
This paper references
10.1046/j.1364-3703.2004.00197.x
Xanthomonas axonopodis pv. citri: factors affecting successful eradication of citrus canker.
J. Graham (2004)
10.1139/m95-145
Growth phase dependent resistance to oxidative stress in a phytopathogen Xanthomonas oryzae pv. oryzae
P. Vattanaviboon (1995)
10.1104/pp.59.2.309
Superoxide dismutases: I. Occurrence in higher plants.
C. N. Giannopolitis (1977)
10.1007/s11738-000-0076-4
The involvement of hydrogen peroxide in plant responses to stresses
E. Kuzniak (2000)
10.1046/j.1364-3703.2003.00163.x
Xanthomonas citri: breaking the surface.
A. Brunings (2003)
10.1016/0024-3205(74)90321-X
Programmed cell death.
R. Lockshin (1974)
10.1023/B:TRAG.0000034610.35724.04
Superoxide Dismutase Transgenes in Sugarbeets Confer Resistance to Oxidative Agents and the Fungus C. beticola
K. Tertivanidis (2004)
10.1094/PDIS.2001.85.4.340
Meeting the challenge of Eradicating Citrus Canker in Florida-Again.
T. Schubert (2001)
10.1046/J.1365-3059.2002.00733.X
Differential induction of superoxide dismutase in downy mildew-resistant and -susceptible genotypes of pearl millet
M. Babitha (2002)
10.1093/emboj/16.16.4806
Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants
H. Willekens (1997)
10.1016/j.micron.2009.12.004
Localisation of hydrogen peroxide accumulation during Solanum tuberosum cv. Rywal hypersensitive response to Potato virus Y.
Katarzyna Otulak (2010)
10.1042/bj3220681
Oxidative burst: an early plant response to pathogen infection.
P. Wojtaszek (1997)
10.1111/j.1364-3703.2008.00486.x
Transcriptional analysis of the sweet orange interaction with the citrus canker pathogens Xanthomonas axonopodis pv. citri and Xanthomonas axonopodis pv. aurantifolii.
R. A. Cernadas (2008)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1371/journal.pone.0010803
The Monofunctional Catalase KatE of Xanthomonas axonopodis pv. citri Is Required for Full Virulence in Citrus Plants
M. Tondo (2010)
10.1093/JEXBOT/53.372.1331
Role of superoxide dismutases (SODs) in controlling oxidative stress in plants.
R. Alscher (2002)
10.1093/jxb/32.1.93
Leaf Senescence: Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation, and Decreased Levels of Superoxide Dismutase and Catalase
R. Dhindsa (1981)
10.1046/J.1365-313X.1997.11061187.X
Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley—powdery mildew interaction
H. Thordal-Christensen (1997)
10.1093/oxfordjournals.pcp.a076232
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Y. Nakano (1981)
10.1073/pnas.93.22.12094
Programmed cell death: a way of life for plants.
J. Greenberg (1996)
10.1016/J.TPLANTS.2004.09.002
Performing the paradoxical: how plant peroxidases modify the cell wall.
F. Passardi (2004)
10.1094/Phyto-82-1319
Penetration through leaf stomata and growth of strains of Xanthomonas campestris in citrus cultivars varying in susceptibility to bacterial diseases
J. Graham (1992)
10.1094/MPMI.1998.11.11.1102
Tobacco class I and II catalases are differentially expressed during elicitor-induced hypersensitive cell death and localized acquired resistance
S. Dorey (1998)
10.1016/J.PMPP.2006.09.001
Molecular cloning and characterization of Gossypium hirsutum superoxide dismutase genes during cotton-Xanthomonas campestris pv. malvacearum interaction
A. E. Voloudakis (2006)
10.1007/s00425-003-1028-1
Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth
A. Liszkay (2003)
10.1038/227680a0
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
10.1093/PCP/PCH165
Ten rice peroxidases redundantly respond to multiple stresses including infection with rice blast fungus.
K. Sasaki (2004)
10.1104/pp.109.150656
Reactive Oxygen Species Are Involved in Plant Defense against a Gall Midge[C][W][OA]
X. Liu (2009)
10.1016/S0168-9452(99)00197-1
Oxidative stress and some antioxidant systems in acid rain-treated bean plants Protective role of exogenous polyamines
V. Velikova (2000)
10.1094/Phyto-82-696
Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks
M. Peng (1992)
10.1104/pp.105.2.467
Active Oxygen Species in Plant Defense against Pathogens
M. Mehdy (1994)
10.1016/0048-4059(83)90019-X
Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components
N. Doke (1983)
10.1016/J.PMPP.2008.03.001
New insights into the resistance of Nagami kumquat to canker disease
Abeer Khalaf (2007)
10.1094/PDIS.2004.88.10.1056
Screening Triploid Hybrids of 'Lakeland' Limequat for Resistance to Citrus Canker.
Z. Viloria (2004)
10.1104/pp.109.4.1247
Changes in Isozyme Profiles of Catalase, Peroxidase, and Glutathione Reductase during Acclimation to Chilling in Mesocotyls of Maize Seedlings
M. D. Anderson (1995)
10.1146/annurev.biochem.77.061606.161055
Cellular defenses against superoxide and hydrogen peroxide.
J. Imlay (2008)
10.1002/PS.706
Role of reactive oxygen species and antioxidants in plant disease resistance.
B. Barna (2003)
10.1105/TPC.10.3.461
Post-Transcriptional Suppression of Cytosolic Ascorbate Peroxidase Expression during Pathogen-Induced Programmed Cell Death in Tobacco
R. Mittler (1998)
10.1023/B:PHYT.0000047795.82713.99
Class III peroxidases and ascorbate metabolism in plants
L. Gara (2004)
10.1094/MPMI.1998.11.11.1038
Apoplastic Peroxidase Generates Superoxide Anions in Cells of Cotton Cotyledons Undergoing the Hypersensitive Reaction to Xanthomonas campestris pv. malvacearum Race 18
Christelle Martinez (1998)
10.1080/14620316.2011.11512726
Superoxide dismutase activity in kumquat leaves infected with Xanthomonas axonopodis pv. citri
N. Kumar (2011)
10.1006/PMPP.1997.0129
Mechanisms for the generation of reactive oxygen species in plant defence – a broad perspective
G. Bolwell (1997)
10.1042/bj2690233
Two temporally synthesized charge subunits interact to form the five isoforms of cottonseed (Gossypium hirsutum) catalase.
W. Ni (1990)
10.1073/PNAS.96.24.14165
Transgenic tobacco plants with reduced capability to detoxify reactive oxygen intermediates are hyperresponsive to pathogen infection.
R. Mittler (1999)
10.1016/S0378-1119(96)00427-1
Regulation of the oxidative stress protective enzymes, catalase and superoxide dismutase in Xanthomonas--a review.
S. Loprasert (1996)
10.1104/pp.98.4.1222
Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves.
I. Cakmak (1992)
10.1139/m95-072
Atypical oxidative stress regulation of a Xanthomonas oryzae pv. oryzae monofunctional catalase
S. Chamnongpol (1995)
10.1104/pp.112.1.433
Correlation of Rapid Cell Death with Metabolic Changes in Fungus-Infected, Cultured Parsley Cells
B. Naton (1996)
10.1105/tpc.10.3.371
Ethylene Regulates the Susceptible Response to Pathogen Infection in Tomato
S. Lund (1998)



This paper is referenced by
10.1111/j.1364-3703.2012.00800.x
The novel virulence-related gene nlxA in the lipopolysaccharide cluster of Xanthomonas citri ssp. citri is involved in the production of lipopolysaccharide and extracellular polysaccharide, motility, biofilm formation and stress resistance.
Q. Yan (2012)
10.1094/MPMI-04-13-0122-R
Increased resistance against citrus canker mediated by a citrus mitogen-activated protein kinase.
M. L. D. de Oliveira (2013)
10.1007/s42161-020-00493-7
New insights into the resistance of four citrus genotypes to Xanthomonas citri subsp. citri
Peng Mei (2020)
10.1038/s41438-020-0278-4
Citron C-05 inhibits both the penetration and colonization of Xanthomonas citri subsp. citri to achieve resistance to citrus canker disease
Hongyan Fu (2020)
10.5772/33853
Interference of Oxidative Metabolism in Citrus by Xanthomonas citri pv citri
Robert C. Ebel (2012)
10.1016/J.SCIENTA.2011.07.005
H2O2 degradation is suppressed in kumquat leaves infected with Xanthomonas axonopodis pv. citri
Nuthan Kumar (2011)
10.1094/MPMI-10-14-0309-R
A Novel Periplasmic Protein, VrpA, Contributes to Efficient Protein Secretion by the Type III Secretion System in Xanthomonas spp.
Xiaofeng Zhou (2015)
10.1007/s11306-017-1163-5
NMR-based metabolomics of transgenic and non-transgenic sweet orange reveals different responses in primary metabolism during citrus canker development
Rafael do Prado Apparecido (2017)
10.1007/s40858-019-00291-2
The effect of drought conditions on sweet orange (Citrus sinensis) plants infected with citrus tristeza virus (CTV)
Camila Cássia Silva (2019)
10.1071/FP18243
Functional study of Csrbohs in defence response against Xanthomonas citri ssp. citri.
Pengying Mei (2019)
Temporal expression of three conserved putative microRNAs in response of Citrus × Limon to Xanthomonas citri subsp . citri and Xanthomonas fuscans subsp .
Aurantifolii (2017)
Semantic Scholar Logo Some data provided by SemanticScholar