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Effects Of Multiple Factors On Hyperhydricity Of Allium Sativum L.

Liu Min, Fangling Jiang, Kong Xiangyu, Jie Tian, Zexiu Wu, Zhanghua Wu
Published 2017 · Biology

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Abstract Hyperhydricity is a common physiological disorder during plant in vitro culture and seriously affected regeneration and micropropagation of plants. Garlic is very susceptible to hyperhydricity. However, effects of multiple factors on hyperhydricity of garlic remain unclear. To clear the regularity of occurrence of hyperhydricity and to obtain a high-efficiency regeneration system of garlic, we systematically investigated effects of explants, media components, culture conditions, and exogenous additives on hyperhydricity. Our results showed that shoots were more easily hyperhydric than plantlets. Shoots induced by inflorescences showed a higher hyperhydric rate and proliferation coefficient than those induced by bulbs. Genotype, physiological age, and explant size affected hyperhydricity of shoots in initial culture, not that of plantlets in subculture. Younger inflorescence and smaller explant were more easily hyperhydric. Dose-dependent manners of cytokinins and gelling agents involved in hyperhydricity were found. Hyperhydricity was aggravated at increased cytokinin concentrations and was alleviated by increased gelling agent and sucrose concentrations, ventilation, and illumination intensity. Media with pH higher than 6.0 and lower than 5.8 resulted in more hyperhydricity. Shoots and plantlets were much more likely to be hyperhydric in MS medium than that in B5 medium. Hyperhydricity was relieved by 50 μM salicylic acid, 250 μM ascorbic acid, 10 μM spermidine, and 50 μM hydrogen peroxide, but aggravated by high concentrations of hydrogen peroxide and spermidine. Mannitol had no effect on hyperhydricity, whereas polyethylene glycol 6000 induced it. Positive correlations of shoot proliferation and hyperhydricity were found under different treatments of cytokinins, gelling agents, and explants which included genotype, organ type, physiology age and size. A regeneration system of garlic with high proliferation coefficient and low hyperhydric rate was established based on the results above.
This paper references
10.1086/336237
Growth and Development of Carnation Shoot Tips In vitro
D. Phillips (1964)
10.1007/s00344-008-9072-5
Growth Conditions in In Vitro Culture Can Induce Oxidative Stress in Mammillaria gracilis Tissues
B. Balen (2008)
10.1007/s10535-008-0001-z
Effects of agar concentration and vessel closure on the organogenesis and hyperhydricity of adventitious carnation shoots
E. Casanova (2008)
10.1080/14620316.2000.11511194
Explant, medium and vessel aeration affect the incidence of hyperhydricity and recovery of normal plantlets in triploid watermelon
P. Thomas (2000)
10.1104/pp.112.202853
Proteome Analysis in Arabidopsis Reveals Shoot- and Root-Specific Targets of Cytokinin Action and Differential Regulation of Hormonal Homeostasis1[W][OA]
M. Žd'árská (2012)
10.1007/s11240-006-9102-6
Differential organ infection studies, potyvirus elimination, and field performance of virus-free garlic plants produced by tissue culture
R. Ramírez-Malagón (2006)
10.1007/s00709-016-0957-z
Induction of reactive oxygen species and the potential role of NADPH oxidase in hyperhydricity of garlic plantlets in vitro
J. Tian (2016)
10.1016/J.TPLANTS.2004.10.002
Biosynthesis of L-ascorbic acid in plants: new pathways for an old antioxidant.
V. Valpuesta (2004)
10.1023/A:1021216324757
Reduction of hyperhydricity in sunflower tissue culture
M. L. Mayor (2004)
10.1007/s10725-006-9139-x
Endogenous cytokinins in shoots of Aloe polyphylla cultured in vitro in relation to hyperhydricity, exogenous cytokinins and gelling agents
M. Ivanova (2006)
10.1007/s11627-015-9698-5
Reduced hyperhydricity in watermelon shoot cultures using silver ions
A. Vinoth (2015)
10.1016/J.JPLPH.2004.07.015
Hyperhydricity in shoot cultures of Scrophularia yoshimurae can be effectively reduced by ventilation of culture vessels.
Chien-chou Lai (2005)
10.1016/J.PLAPHY.2005.05.006
Reducing properties, energy efficiency and carbohydrate metabolism in hyperhydric and normal carnation shoots cultured in vitro: a hypoxia stress?
Shady Saher (2005)
10.1007/s11627-001-0044-8
Control of hyperhydricity in micropropagated apricot cultivars
O. Pérez-Tornero (2001)
10.1007/s11240-007-9311-7
Effect of ammonium ions and cytokinins on hyperhydricity and multiplication rate of in vitro regenerated shoots of Aloe polyphylla
M. Ivanova (2007)
10.1093/TREEPHYS/26.3.377
Hyperhydricity in apple: ultrastuctural and physiological aspects
D. Chakrabarty (2006)
10.1007/s11240-009-9589-8
Nitrogen source, concentration, and NH4+:NO3− ratio influence shoot regeneration and hyperhydricity in tissue cultured Aloe polyphylla
M. Ivanova (2009)
10.1007/s11627-015-9727-4
From leaf explants to rooted plantlets in a mist reactor
Liwen Fei (2015)
10.1105/tpc.105.033589
Redox Homeostasis and Antioxidant Signaling: A Metabolic Interface between Stress Perception and Physiological Responses
C. Foyer (2005)
10.1007/s11240-011-9964-0
Cytokinin and explant types influence in vitro plant regeneration of Leopard Orchid (Ansellia africana Lindl.)
R. Vasudevan (2011)
10.1016/S0168-9452(00)00463-5
Hyperhydricity in in vitro eggplant regenerated plants: structural characteristics and involvement of BiP (Binding Protein).
E. A. Picoli (2001)
10.1007/s11240-010-9794-5
Influence of gelling agent and cytokinins on the control of hyperhydricity in Aloe polyphylla
M. Ivanova (2010)
10.3389/fpls.2016.00029
Nitrogen Source and External Medium pH Interaction Differentially Affects Root and Shoot Metabolism in Arabidopsis
Asier Sarasketa (2016)
10.1139/CJPS10034
Influencing factors and structural characterization of hyperhydricity of in vitro regeneration in Brassica oleracea var. italica
Y. Yu (2011)
10.1111/J.1399-3054.1991.TB02904.X
Effects of agar concentration on water status and growth of rose plants cultured in vitro
Jaleh Ghashghaie (1991)
10.1007/s11627-008-9180-8
Analysis of ultrastructure and reactive oxygen species of hyperhydric garlic (Allium sativum L.) shoots
Z. Wu (2008)
10.1016/J.SCIENTA.2012.02.005
Increased light intensity during in vitro culture improves water loss control and photosynthetic performance of Castanea sativa grown in ventilated vessels.
P. Sáez (2012)
10.1007/s11240-014-0568-3
Effect of polyamines and polyamine precursors on hyperhydricity in micropropagated apple shoots
J. Tabart (2014)
10.1016/J.ENVEXPBOT.2007.08.004
Sub-cellular location of H2O2, peroxidases and pectin epitopes in control and hyperhydric shoots of carnation
N. Fernández-García (2008)
10.1016/j.plantsci.2016.10.008
Reversion of hyperhydricity in pink (Dianthus chinensis L.) plantlets by AgNO3 and its associated mechanism during in vitro culture.
Hongyang Gao (2017)
10.1016/0304-4238(94)90144-9
In vitro propagation of Paeonia suffruticosa Andr. cv. ‘Mme de Vatry’: developmental effects of exogenous hormones during the multiplication phase
Lydia Bouza (1994)
10.1016/J.PLAPHY.2004.05.003
Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response.
T. Franck (2004)
10.1023/B:TICU.0000016825.18930.e4
Hyperhydricity of Micropropagated Shoots: A Typically Stress-induced Change of Physiological State
C. Kevers (2004)
10.1023/A:1022236920605
Development of suitable protocol to overcome hyperhydricity in carnation during micropropagation
M. Yadav (2004)
10.1016/S0304-4238(03)00097-9
Effect of sealed and vented gaseous microenvironments on the hyperhydricity of potato shoots in vitro
S. W. Park (2004)
10.1016/j.plaphy.2011.10.006
SA improvement of hyperhydricity reversion in Thymus daenensis shoots culture may be associated with polyamines changes.
Sahar Hassannejad (2012)
10.3945/jn.114.202333
Garlic and Heart Disease.
Ravi Varshney (2016)
10.1007/s10725-009-9430-8
Natural ventilation effectively reduces hyperhydricity in shoot cultures of Aloe polyphylla Schönland ex Pillans
M. Ivanova (2009)
Introduction to plant tissue culture
M. K. Razdan (2003)
10.4141/CJPS10034
Influencing factors and structural characterization of hyperhydricity of in vitro regeneration in Brassica oleracea var. italica
Yuya (2011)
10.1023/A:1022378511659
Effects of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots
M. Kadota (2004)
10.1007/s11627-015-9700-2
The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines
F. Bernard (2015)
10.1007/BF00193716
In vitro culture of Doritaenopsis: comparison between formation of the hyperhydric protocorm-like-body (PLB) and the normal PLB
Tian-Su Zhou (2004)
10.1007/s00344-015-9569-7
Proteomic and Antioxidant Analysis Elucidates the Underlying Mechanism of Tolerance to Hyperhydricity Stress in In Vitro Shoot Cultures of Dianthus caryophyllus
S. Muneer (2015)
10.3945/jn.114.202192
Garlic Lowers Blood Pressure in Hypertensive Individuals, Regulates Serum Cholesterol, and Stimulates Immunity: An Updated Meta-analysis and Review.
K. Ried (2016)
10.1007/s11627-013-9511-2
Antioxidant enzyme activities, malondialdehyde, and total phenolic content of PEG-induced hyperhydric leaves in sugar beet tissue culture
A. Şen (2013)
10.1007/s11240-004-4043-4
Prevention of hyperhydricity in micropropagated carnation shoots by bottom cooling: implications of oxidative stress
Sadhy Saher (2004)
10.3835/plantgenome2015.09.0080
Global Transcriptome Analysis Reveals Differences in Gene Expression Patterns Between Nonhyperhydric and Hyperhydric Peach Leaves
Y. Bakır (2016)
10.1016/S0168-9452(02)00048-1
Effects of the culture medium pH and ion uptake in in vitro vegetative organogenesis in thin cell layers of tobacco
G. Pasqua (2002)
10.1007/s11240-015-0780-9
Selection and validation of garlic reference genes for quantitative real-time PCR normalization
M. Liu (2015)
10.1007/s11240-006-9148-5
Effects of explants and growth regulators in garlic callus formation and plant regeneration
G. Luciani (2006)
10.1079/IVP2006791
Influence of ventilation closure, gelling agent and explant type on shoot bud proliferation and hyperhydricity in Scrophularia yoshimurae—A medicinal plant
H. Tsay (2007)
10.1007/s11240-014-0623-0
The apoplastic oxidative burst as a key factor of hyperhydricity in garlic plantlet in vitro
J. Tian (2014)
10.1111/J.1745-4514.1999.TB00590.X
INFLUENCE OF ACETYL SALICYLIC ACID IN COMBINATION WITH FISH PROTEIN HYDROLYSATES ON HYPERHYDRICITY REDUCTION AND PHENOLIC SYNTHESIS IN OREGANO (ORIGANUM VULGARE) TISSUE CULTURES
N. Andarwulan (1999)
10.1007/s11627-013-9491-2
Phloroglucinol in plant tissue culture
Jaime A. Teixeira da Silva (2013)
10.1007/s004970000061
Floral development in bolting garlic
R. Kamenetsky (2001)
10.1038/srep22805
Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans
Wen-ru Li (2016)
10.1016/J.PLANTSCI.2003.12.039
Relationship between osmotic stress and the levels of free, conjugated and bound polyamines in leaves of wheat seedlings
H. Liu (2004)
10.1104/PP.116.4.1403
Root growth and oxygen relations at low water potentials. Impact Of oxygen availability in polyethylene glycol solutions
Verslues (1998)
10.1016/J.ENVEXPBOT.2005.06.019
Lipid peroxidation and antioxidant enzyme activities of Euphorbia millii hyperhydric shoots
Y. H. Dewir (2006)
10.1016/S0304-4238(00)00234-X
Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear
M. Kadota (2001)
10.1590/S1516-8913201402165
Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.
M. P. Machado (2014)
10.1073/pnas.171304098
Regulation of plant growth by cytokinin
T. Werner (2001)
10.1111/J.1365-3040.1995.TB00575.X
The different effects of PEG 6000 and NaCI on leaf development are associated with differential inhibition of root water transport
O. Chazen (1995)
10.1007/BF00716673
Vitrification and soluble carbohydrate levels inPetunia leaves as influenced by media gelrite and sucrose concentrations
T. W. Zimmerman (2004)
10.1016/J.PBI.2007.08.008
Salicylic acid in plant defence--the players and protagonists.
G. Loake (2007)
10.1023/A:1010650131732
Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation
J. Majada (2004)
10.1007/BF00048762
Transgenic carnation plants obtained by Agrobacterium tumefaciens-mediated transformation of petal explants
A. V. Altvorst (2004)
10.1093/jxb/ert315
Flooding of the apoplast is a key factor in the development of hyperhydricity
Niels van den Dries (2013)
10.1016/S0032-9592(98)00011-9
Reduced hyperhydricity and enhanced growth of tissue culture-generated raspberry (Rubus sp.) clonal lines by Pseudomonas sp. isolated from oregano
K. Ueno (1998)



This paper is referenced by
10.3389/fpls.2017.00738
Exogenous Supplementation of Silicon Improved the Recovery of Hyperhydric Shoots in Dianthus caryophyllus L. by Stabilizing the Physiology and Protein Expression
P. Soundararajan (2017)
10.1007/s11627-019-10038-y
Evaluating a DOE screen to reduce hyperhydricity in the threatened plant, Cycladenia humilis var. jonesii
V. Pence (2020)
10.24326/asphc.2020.4.4
EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS
Tour Jan (2020)
10.15159/AR.20.039
In vitro effects of regulators on growth and morphogenesis of Ocimum basilicum L. ‘Alfavaca Green’ stem apexes
H. M. Magalhães (2020)
10.15835/NBHA48311926
Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica
G. Vlachou (2020)
10.1515/fhort-2017-0022
A robust method for haploid sugar beet in vitro proliferation and hyperhydricity reduction
Arman Pazuki (2017)
10.5010/JPB.2018.45.4.382
In vitro micropropagation of M.26 ( Malus pumila Mill) apple rootstock and assessment of the genetic diversity of proliferated plantlets using simple sequence repeat markers
K. H. Cho (2018)
10.1016/J.SAJB.2020.08.019
Light-emitting diodes and gas exchange facilitation minimize hyperhydricity in Lippia grata: Physiological, biochemical and morpho anatomical aspects
Jhonatan Rafael Zárate-Salazar (2020)
10.1007/s11627-020-10075-y
Effects of culture medium composition and PEG on hyperhydricity in Dendrobium officinale
Hongyang Gao (2020)
10.1016/j.sajb.2019.11.022
Micropropagation and prevention of hyperhydricity in olive (Olea europaea L.) cultivar 'Gemlik'
M. Bayraktar (2020)
10.1007/s11627-020-10111-x
Optimization of efficient direct organogenesis protocol for Punica granatum L. cv. Kandhari Kabuli from mature leaf explants
Vipasha Verma (2020)
10.1016/J.SCIENTA.2017.07.042
System construction of virus-free and rapid-propagation technology of Baodi garlic (Allium sativum L.)
Fan Baoli (2017)
Use of meta-Topolin, an unconventional cytokinin in the in vitro multiplication of Opuntia stricta Haw
L. Souza (2019)
10.1007/S12298-020-00885-0
Influence of polyamines on hyperhydricity reversion and its associated mechanism during micropropagation of China pink (Dianthus chinensis L.).
R. Sreelekshmi (2020)
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