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A Two-photon Fluorescent Probe For Visualizing Endoplasmic Reticulum Peroxynitrite In Parkinson's Disease Models

Ming Yan, Haixiao Fang, Xiaoqing Wang, Jiajia Xu, C. Zhang, L. Xu, L. Lin
Published 2021 · Chemistry

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Abstract Endoplasmic reticulum (ER) stress is implicated in the pathogenesis of diseases such as Parkinson's disease (PD). Peroxynitrite is the pivotal causative factor of ER stress. However, fluorescent probes for imaging the peroxynitrite in the ER are still lacking. In the present paper, we reported the design and synthesis of a two-photon fluorescence probe (ER-PN) for visualizing the ER peroxynitrite in PD models of living cells and C. elegans. ER-PN showed a rapidly and highly selective “turn-on” response to peroxynitrite over other biological species in vitro. Moreover, ER-PN showed excellent ER targeting ability and worked well in living systems for the ER peroxynitrite imaging. Noteworthy, ER-PN has been successfully applied for imaging the peroxynitrite in PD models, indicating its potential application for revealing the fundamental roles of peroxynitrite in ER stress-related diseases.
This paper references
10.1021/acs.jafc.9b01298
Novel Dimethylhydrazine-Derived Spirolactam Fluorescent Chemodosimeter for Tracing Basal Peroxynitrite in Live Cells and Zebrafish.
Caiyun Liu (2019)
10.1021/acs.analchem.6b01391
Highly Selective and Sensitive One- and Two-Photon Ratiometric Fluorescent Probe for Intracellular Hydrogen Polysulfide Sensing.
Qingxin Han (2016)
10.1021/acs.analchem.9b01511
High Spatiotemporal Resolution Observation of Glutathione Hydropersulfides in Living Cells and Tissue via a Two-Photon Ratiometric Fluorescent Probe.
Y. Wang (2019)
10.1021/acs.accounts.9b00302
Reaction-Based Fluorescent Probes for the Detection and Imaging of Reactive Oxygen, Nitrogen, and Sulfur Species
Luling Wu (2019)
10.1016/j.brainres.2016.04.042
ER stress and Parkinson's disease: Pathological inputs that converge into the secretory pathway
Gabriela Mercado (2016)
10.1021/jacs.5b06865
Visualizing peroxynitrite fluxes in endothelial cells reveals the dynamic progression of brain vascular injury.
X. Li (2015)
10.1016/j.snb.2020.128211
A two-photon fluorescent probe for HNO rapid visualization in endoplasmic reticulum
Peng Shuxin (2020)
10.1021/acs.analchem.8b03207
Mitochondrial Peroxynitrite Mediation of Anthracycline-Induced Cardiotoxicity as Visualized by a Two-Photon Near-Infrared Fluorescent Probe.
Xilei Xie (2018)
10.1016/J.SNB.2018.01.203
A simple highly selective and sensitive hydroquinone-based two-photon fluorescent probe for imaging peroxynitrite in live cells
B. Zhu (2018)
10.1021/jacs.6b06398
FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5.
Xiaotong Jia (2016)
10.1021/acs.analchem.9b03024
Mito-Specific Ratiometric Terbium(III) Complex-Based Luminescence Probe for Accurate Detection of Endogenous Peroxynitrite by Time-Resolved Luminescence Assay.
Yingzhe Wang (2019)
10.1021/acs.analchem.9b00317
Peroxynitrite Activatable NIR-II Fluorescent Molecular Probe for Drug-Induced Hepatotoxicity Monitoring.
D. Li (2019)
10.1021/acs.analchem.8b01960
A Two-Photon Fluorescent Probe for Imaging Endogenous ONOO- near NMDA Receptors in Neuronal Cells and Hippocampal Tissues.
D. Lee (2018)
10.1039/c9cc02483b
In situ visualization of peroxisomal peroxynitrite in the livers of mice with acute liver injury induced by carbon tetrachloride using a new two-photon fluorescent probe.
Yongqing Zhou (2019)
10.1039/c9tb02919b
Revealing the redox status in endoplasmic reticulum by a selenium fluorescence probe.
Shunping Zang (2020)
10.1016/J.SNB.2018.08.045
Imaging viscosity and peroxynitrite by a mitochondria-targeting two-photon ratiometric fluorescent probe
W. Sun (2018)
10.1039/c6sc00012f
A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues† †Electronic supplementary information (ESI) available: Detailed experimental procedures for the organic synthesis, photophysical characterization and supplementary imaging figure
T. Peng (2016)
10.1016/J.CEB.2004.09.012
Misfolded proteins, endoplasmic reticulum stress and neurodegeneration.
R. Rao (2004)
10.3390/ijms14010434
An Involvement of Oxidative Stress in Endoplasmic Reticulum Stress and Its Associated Diseases
Bidur Bhandary (2012)
10.1089/ARS.2009.2625
Mechanisms and implications of reactive oxygen species generation during the unfolded protein response: roles of endoplasmic reticulum oxidoreductases, mitochondrial electron transport, and NADPH oxidase.
C. Santos (2009)
10.1021/jacs.6b10508
Selective Visualization of the Endogenous Peroxynitrite in an Inflamed Mouse Model by a Mitochondria-Targetable Two-Photon Ratiometric Fluorescent Probe.
Dan Cheng (2017)
10.1021/acschemneuro.7b00176
Gut Microbiota, Nitric Oxide, and Microglia as Prerequisites for Neurodegenerative Disorders.
Joyce K Y Tse (2017)
10.1039/c9sc03798e
Physicochemical-property guided design of a highly sensitive probe to image nitrosative stress in the pathology of stroke
Juan Cheng (2020)
10.1021/acs.analchem.7b02059
Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues.
J. Li (2017)
10.1021/acs.analchem.7b00503
Observation of the Generation of ONOO- in Mitochondria under Various Stimuli with a Sensitive Fluorescence Probe.
H. Li (2017)
10.1039/c8cc04919j
ESIPT-based ratiometric fluorescence probe for the intracellular imaging of peroxynitrite.
Luling Wu (2018)
10.1089/ars.2017.7144
Neuroprotection in Oxidative Stress-Related Neurodegenerative Diseases: Role of Endocannabinoid System Modulation.
J. Pálóczi (2018)
10.1089/ars.2014.5851
Endoplasmic reticulum stress and oxidative stress in cell fate decision and human disease.
Stewart Siyan Cao (2014)
10.1016/j.toxlet.2020.01.019
Endoplasmic reticulum stress, an important factor in the development of Parkinson's disease.
Zheng Mou (2020)
10.1021/acs.analchem.7b01659
Efficient Two-Photon Fluorescent Probe for Glutathione S-Transferase Detection and Imaging in Drug-Induced Liver Injury Sample.
J. Zhang (2017)
10.1146/ANNUREV.BI.56.070187.004145
Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi.
S. Pfeffer (1987)
10.1016/J.SNB.2017.12.135
A highly selective and ultrasensitive ratiometric far-red fluorescent probe for imaging endogenous peroxynitrite in living cells
B. Zhu (2018)
10.1039/C7TB02615C
A lysosome targetable versatile fluorescent probe for imaging viscosity and peroxynitrite with different fluorescence signals in living cells.
Bingpeng Guo (2018)
10.1002/advs.201900341
Imaging Dynamic Peroxynitrite Fluxes in Epileptic Brains with a Near‐Infrared Fluorescent Probe
Jiong‐sheng Hu (2019)
10.1021/acs.iecr.9b03854
Dicyanoisophorone-Derived Near-Infrared Fluorescent Probe for Ultrasensitive Detection of Peroxynitrite in Living Cells and Zebrafish
P. Jia (2019)
10.1021/ol102182j
HKGreen-3: a rhodol-based fluorescent probe for peroxynitrite.
T. Peng (2010)
10.1007/s10072-016-2663-1
Oxidative and nitrosative stress in serum of patients with Parkinson’s disease
H. Çubukçu (2016)
10.1021/acs.analchem.9b04381
Glutathione peroxidase-activatable two-photon ratiometric fluorescent probe for redox mechanism research in aging and mercury exposure mice models.
Yue Wang (2019)
10.1039/c8cc08061e
A ruthenium(ii) complex-cyanine energy transfer scaffold based luminescence probe for ratiometric detection and imaging of mitochondrial peroxynitrite.
Wenzhu Zhang (2018)
10.1021/acschembio.8b00535
Targeting Fluorescent Sensors to Endoplasmic Reticulum Membranes Enables Detection of Peroxynitrite During Cellular Phagocytosis.
Kelsey E. Knewtson (2018)
10.1083/jcb.200311055
Oxidative protein folding in eukaryotes
B. Tu (2004)
10.1016/J.CCLET.2019.08.001
Recent progress in two-photon small molecule fluorescent probes for enzymes
D. Chen (2019)
10.1073/pnas.1804932115
Oxygen radicals, nitric oxide, and peroxynitrite: Redox pathways in molecular medicine
R. Radi (2018)
10.1016/J.DYEPIG.2019.04.054
Tuning the photo-physical properties of BODIPY dyes: Effects of 1, 3, 5, 7- substitution on their optical and electrochemical behaviours
Jiayu Tao (2019)
10.1016/J.SNB.2017.12.205
Two-photon fluorescence probe for precisely detecting endogenous H2S in lysosome by employing a dual lock system
Juan Ouyang (2018)
10.1039/c8cc90499e
Correction: A ratiometric fluorescent probe for peroxynitrite prepared by de novo synthesis and its application in assessing the mitochondrial oxidative stress status in cells and in vivo.
Dong-Ye Zhou (2018)
10.1016/J.CCLET.2018.04.007
Recent progress in small molecule fluorescent probes for nitroreductase
Wenjing Qin (2018)
10.1021/JA0603756
A highly selective fluorescent probe for the detection and imaging of peroxynitrite in living cells.
D. Yang (2006)
10.1161/HH1501.094365
Methods of detection of vascular reactive species: nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite.
M. Tarpey (2001)
10.1021/acs.analchem.0c00259
A Lysosome-Targeting Iridium(III) Probe with Near-Infrared Emission for The Visualization of NO/O2•- Crosstalk via in vivo Peroxynitrite Imaging.
W. Wu (2020)
10.1016/j.saa.2020.118680
A near-infrared fluorescent probe for imaging of nitroxyl in living cells.
Z. Liu (2020)
10.1016/J.SNB.2017.10.170
A highly specific far-red fluorescent probe for imaging endogenous peroxynitrite in the mitochondria of living cells
B. Zhu (2018)
10.1080/15321819.2019.1583109
Peroxynitrite: cellular pathology and implications in autoimmunity
Rizwan Ahmad (2019)
10.3389/fncel.2014.00213
A Molecular Web: Endoplasmic Reticulum Stress, Inflammation, and Oxidative Stress
N. Chaudhari (2014)
10.1021/acs.analchem.9b05599
Ultrafast Detection of Peroxynitrite in Parkinson's Disease Models Using a Near-Infrared Fluorescent Probe.
Qian Sun (2020)
10.1021/acs.chemrev.7b00568
Biochemistry of Peroxynitrite and Protein Tyrosine Nitration.
G. Ferrer-Sueta (2018)
10.1021/ja504624q
Molecular imaging of peroxynitrite with HKGreen-4 in live cells and tissues.
T. Peng (2014)
10.1016/J.DYEPIG.2019.02.054
A reversible “turn-off-on” fluorescent probe for real-time visualization of mercury(II) in environmental samples and its biological applications
F. Song (2019)
10.1039/c4cc09122a
A mitochondria-targetable fluorescent probe for peroxynitrite: fast response and high selectivity.
H. Zhang (2015)
Nitric oxide and peroxynitrite serum levels in Parkinson's disease: correlation of oxidative stress and the severity of the disease.
L. Kouti (2013)
10.1161/01.ATV.0000189159.96900.d9
Peroxynitrite Causes Endoplasmic Reticulum Stress and Apoptosis in Human Vascular Endothelium: Implications in Atherogenesis
J. Dickhout (2005)
10.1021/acs.analchem.8b03565
Endoplasmic Reticulum-Targeted Ratiometric N-Heterocyclic Carbene Borane Probe for Two-Photon Microscopic Imaging of Hypochlorous Acid.
Yen Leng Pak (2018)



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