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Novel Colorimetric Immunoassay For The Detection Of Aflatoxin B1

S. Garden, N. Strachan
Published 2001 · Chemistry

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Abstract A rapid colorimetric sequential injection immunoassay (SIIA) system utilising a jet ring flow cell packed with a solid phase of polymethylmethacrylate beads was used for the detection of aflatoxin B 1 (AFB 1 ). This method is proven in its ability to detect AFB 1 down to a level of 0.2 ng ml −1 in artificially contaminated food materials, which is comparable to the sensitivity of microtitre plate ELISA. The automated SIIA system takes under 10 min per sample and uses a methanol–water (80:20) and hexane extraction procedure that takes approximately 15 min to perform.
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