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Cholera Toxin-induced Modulation Of Gene Expression: Elucidation Via CDNA Microarray For Rational Cell-based Sensor Design

H. J. Lin, P. Charles, J. Andreadis, A. Churilla, D. Stenger, J. Pancrazio
Published 2002 · Chemistry

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Abstract Cell-based biosensors utilize functional changes in cellular response to identify the biological threats in a physiological relevant manner. Cell-based sensors have been used for a wide array of applications including toxicological assessment and drug-screening. In this paper, we utilize DNA arrays to identify differential gene expression events induced by toxin exposure for the purpose of developing a reporter gene assay system compatible with insertion into a cell-based sensor platform. HT29, an intestine epithelial cell line, was used as a cell model to study the cholera toxin (CT)-induced host cell modulation using DNA array analysis. A false positive model was generated from analysis of housekeeping genes in untreated control experiments to characterize our system and to minimize the number of false positives in the data. Threshold probability scores (−3.72), which gives
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