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Cholera Toxin-induced Modulation Of Gene Expression: Elucidation Via CDNA Microarray For Rational Cell-based Sensor Design
H. J. Lin, P. Charles, J. Andreadis, A. Churilla, D. Stenger, J. Pancrazio
Published 2002 · Chemistry
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Abstract Cell-based biosensors utilize functional changes in cellular response to identify the biological threats in a physiological relevant manner. Cell-based sensors have been used for a wide array of applications including toxicological assessment and drug-screening. In this paper, we utilize DNA arrays to identify differential gene expression events induced by toxin exposure for the purpose of developing a reporter gene assay system compatible with insertion into a cell-based sensor platform. HT29, an intestine epithelial cell line, was used as a cell model to study the cholera toxin (CT)-induced host cell modulation using DNA array analysis. A false positive model was generated from analysis of housekeeping genes in untreated control experiments to characterize our system and to minimize the number of false positives in the data. Threshold probability scores (−3.72), which gives
This paper references
Particulate matter initiates inflammatory cytokine release by activation of capsaicin and acid receptors in a human bronchial epithelial cell line.
B. Veronesi (1999)
Expression of stably transfected murine glutathione S-transferase A3-3 protects against nucleic acid alkylation and cytotoxicity by aflatoxin B1 in hamster V79 cells expressing rat cytochrome P450-2B1.
W. Fields (1999)
Heat shock and cytokines modulate the expression of adhesion molecules on different human gastric‐cancer cell lines
M. Hsieh (1996)
Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor.
L. Cao (1995)
Discovery of differentially expressed genes associated with paclitaxel resistance using cDNA array technology: analysis of interleukin (IL) 6, IL-8, and monocyte chemotactic protein 1 in the paclitaxel-resistant phenotype.
Z. Duan (1999)
Enhancing effect of cholera toxin on interleukin-6 secretion by IEC-6 intestinal epithelial cells: mode of action and augmenting effect of inflammatory cytokines.
D. McGee (1993)
Portable cell-based biosensor system for toxin detection
J. Pancrazio (1998)
Analysis of p53-regulated gene expression patterns using oligonucleotide arrays.
R. Zhao (2000)
The 5'-flanking region of the human P-450(SCC) gene shows responsiveness to cAMP-dependent regulation in a transient gene-expression system of Y-1 adrenal tumor cells.
H. Inoue (1988)
Cholera Toxin Enhances Alloantigen Presentation by Cultured Intestinal Epithelial Cells
A. Bromander (1993)
p53-dependent G2 arrest associated with a decrease in cyclins A2 and B1 levels in a human carcinoma cell line
C. Badie (2000)
Odor, drug and toxin analysis with neuronal networks in vitro: extracellular array recording of network responses.
G. Gross (1997)
Molecular Cloning of a Gene Encoding a New Type of Metalloproteinase-disintegrin Family Protein with Thrombospondin Motifs as an Inflammation Associated Gene*
K. Kuno (1997)
Sensitivity issues in DNA array-based expression measurements and performance of nylon microarrays for small samples.
F. Bertucci (1999)
A Frequency Domain Approach to Nonlinear and Structure Identification for Long Memory Systems: Application to Lung Mechanics
Qin Zhang (2004)
Detection of peroxisome proliferators using a reporter construct derived from the rat acyl-CoA oxidase promoter in the rat liver cell line H-4-II-E.
M. Lee (1997)
Regulation of apolipoprotein E biosynthesis by cAMP and phorbol ester in rat ovarian granulosa cells.
K. Wyne (1989)
Cholera toxin and Salmonella typhimurium induce different cytokine profiles in the gastrointestinal tract.
G. Klimpel (1995)
Identification of new members of the Gas2 and Ras families in the 22q12 chromosome region.
J. Zucman-Rossi (1996)
The use of neuronal networks on multielectrode arrays as biosensors.
G. Gross (1995)
Structure–function analysis of an evolutionary conserved protein, DAP3, which mediates TNF‐α‐ and Fas‐induced cell death
J. Kissil (1999)
Cholera Toxin B-Mediated Targeting of Lipid Vesicles Containing Ganglioside GM1 to Mucosal Epithelial Cells
T. Lian (2004)
Cloning and Characterization of the Murine Nek3 Protein Kinase, a Novel Member of the NIMA Family of Putative Cell Cycle Regulators*
K. Tanaka (1999)
Large-scale monitoring of host cell gene expression during HIV-1 infection using cDNA microarrays.
G. Geiss (2000)
Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas.
A. Wellmann (2000)
Comparing functional genomic datasets: lessons from DNA microarray analyses of host-pathogen interactions.
M. Diehn (2001)
cDNA cloning of a novel human gene NAKAP95, neighbor of A-kinase anchoring protein 95 (AKAP95) on chromosome 19p13.11–p13.12 region
N. Seki (2000)
The Immunophilin-like Protein XAP2 Regulates Ubiquitination and Subcellular Localization of the Dioxin Receptor*
A. Kazlauskas (2000)
Using the metabolism of PAHs in a human cell line to characterize environmental samples.
Discovery of New Inducible Genes in in vitroDecidualized Human Endometrial Stromal Cells Using Microarray Technology.
Rozana M Popovici (2000)
beta1,6 N-acetylglucosaminyltransferase (core 2 GlcNAc-T) expression in normal rat tissues and different cell lines: evidence for complex mechanisms of regulation.
I. Vanderelst (1998)
Cloning and characterization of two mouse genes with homology to the yeast Sir2 gene.
Y. Yang (2000)
The interaction of intestinal epithelial cells and intraepithelial lymphocytes in host defense
Y. Yoshikai (1999)
DNA chips: An array of possibilities
A. Marshall (1998)
Analysis by High Density cDNA Arrays of Altered Gene Expression in Human Intestinal Epithelial Cells in Response to Infection with the Invasive Enteric BacteriaSalmonella *
L. Eckmann (2000)
Transcriptional regulation of the human UDP-GlcNAc: alpha-6-D-mannoside beta-1-2-N-acetylglucosaminyltransferase II gene (MGAT2) which controls complex N-glycan synthesis
S. Chen (2004)
Estrogenic effects of organochlorine pesticides on uterine leiomyoma cells in vitro.
L. C. Hodges (2000)
Oxidative injury mediated by the hepatic cytochrome P-450 system in conjunction with cellular iron. Effects on the pathway of haem biosynthesis.
A. Smith (1990)
Chromatin assembly during DNA replication in somatic cells.
T. Krude (1999)
Regulation of the mannose 6‐phosphate/IGF II receptor expression at the cell surface by mannose 6‐phosphate, insulin like growth factors and epidermal growth factor.
T. Braulke (1989)
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
M. Schena (1995)
The Ran‐GTPase and cell‐cycle control
J. Moore (2001)
Expression scanning of an array of growth control genes in human tumor cell lines
F. Bertucci (1999)
Fluorescent cDNA microarray hybridization reveals complexity and heterogeneity of cellular genotoxic stress responses
S. Amundson (1999)
Use of a cDNA microarray to analyse gene expression patterns in human cancer
J. DeRisi (1996)
A bioassay for cholera-like toxins using HT29 cells
Zeta Charania (1991)
Detection of Clostridium botulinum toxin A using a fiber optic-based biosensor.
R. A. Ogert (1992)
cDNA expression array reveals heterogeneous gene expression profiles in three glioblastoma cell lines
C. H. Rhee (1999)
Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.
E. Fairey (1999)
Maturation of human monocyte-derived dendritic cells studied by microarray hybridization.
A. Dietz (2000)
Apoptotic signalling cascade in photosensitized human epidermal carcinoma A431 cells: involvement of singlet oxygen, c-Jun N-terminal kinase, caspase-3 and p21-activated kinase 2.
W. H. Chan (2000)
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Angiogenic tube formation of bovine aortic endothelial cells grown on patterns formed by H2/He plasma treatment of the plasma polymerized hexamethyldisiloxane film.
J. Park (2015)
Cellular impedance biosensors for drug screening and toxin detection.
Fareid Asphahani (2007)
Selective adhesion of intestinal epithelial cells on patterned films with amine functionalities formed by plasma enhanced chemical vapor deposition
K. S. Kim (2010)
Biological threat detection via host gene expression profiling.
B. Lin (2003)
Novel Cell-based Biosensors Based on Measurement of Fluorescence: Novel Cell-based Biosensors Based on Measurement of Fluorescence
Wen-Wen Xin (2011)
Identification of target genes responsive to JP-8 exposure in the rat central nervous system
B. Lin (2001)
Development of Biosensors for the Detection of Biological Warfare Agents: Its Issues and Challenges
H. Kumar (2013)
Comparison of Cell-Based Biosensors with Traditional Analytical Techniques for Cytotoxicity Monitoring and Screening of Polycyclic Aromatic Hydrocarbons in the Environment
A. Ahmad (2009)
Recent advances in analytical chemistry--a material approach.
Lin He (2006)
Biosensor technology for detecting biological warfare agents : Recent progress and future trends
J. Gooding (2006)
Portable array biosensors
C. R. Taitt (2003)
Current Awareness on Comparative and Functional Genomics
B. Shlomo (2002)
Comparative and Functional Genomics Vol 3 No 6
Guided cell patterning on gold-silicon dioxide substrates by surface molecular engineering.
Mandana Veiseh (2004)