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Identification Of A Silencing Element In The Chicken Lipoprotein Lipase Gene Promoter: Characterization Of The Silencer-binding Protein And Delineation Of Its Target Nucleotide Sequence.

W. Zhang, A. Bensadoun
Published 1999 · Biology, Medicine

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Lipoprotein lipase (LPL) hydrolyzes triglycerides in chylomicrons and very low density lipoproteins (VLDL) and plays a central role in lipid metabolism. It is regulated tissue-specifically. By deletion analysis, a negative regulatory element was identified in the chicken LPL gene promoter at base pairs (bp) -263 to -241. This sequence contained two palindromic halves with a three nucleotide spacer. Either half was sufficient for full inhibitory function. A protein complex bound specifically to this element and a high correlation was found between binding of the complex and inhibition of transcription. Its molecular mass, evaluated by native gel electrophoresis and Ferguson plot analysis, was 120 kDa. UV cross-linking followed by SDS-PAGE revealed two protein subunits of 48 kDa and 44 kDa, respectively. This inhibitory protein complex may contribute to the tissue-specific regulation of LPL gene transcription. It was much more abundant in liver than in adipose tissue and heart. Our data showed that this negative element inhibited transcription even when placed at an upstream location (-666), but failed to function in the herpes simplex virus thymidine kinase promoter, indicating that it acted in conjunction with other element(s) in the chicken LPL gene to inhibit transcription.
This paper references
Characterization of drosophila transcription factors that activate the tandem promoters of the alcohol dehydrogenase gene
U. Heberlein (1985)
Transcriptional regulation of the human lipoprotein lipase gene in 3T3-L1 adipocytes.
L. Previato (1991)
Characterization of the human lipoprotein lipase (LPL) promoter: evidence of two cis-regulatory regions, LP-alpha and LP-beta, of importance for the differentiation-linked induction of the LPL gene during adipogenesis.
S. Enerbäck (1992)
Structure of the human lipoprotein lipase gene.
S. Deeb (1989)
SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis
X. Wang (1994)
Lipoprotein lipase and hepatic lipase mRNA tissue specific expression, developmental regulation, and evolution.
C. Semenkovich (1989)
Glucocorticoid responsiveness of the transcriptional enhancer of Moloney Murine Sarcoma Virus
R. Miksicek (1986)
Opposite regulation of gene transcription and cell proliferation by c-Myc and Max.
W. Gu (1993)
Synthesis of lipoprotein lipase in the liver of newborn rats and localization of the enzyme by immunofluorescence.
S. Vilaró (1988)
Identification of the 5' regulatory elements of avian lipoprotein lipase gene: synergistic effect of multiple factors.
S. Lu (1993)
Transcriptional repression of the c-fos gene by YY1 is mediated by a direct interaction with ATF/CREB.
Q. Zhou (1995)
Tissue-specific in vitro transcription from the mouse albumin promoter
K. Górski (1986)
An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter
R. Carthew (1985)
PPARalpha and PPARgamma activators direct a distinct tissue‐specific transcriptional response via a PPRE in the lipoprotein lipase gene.
K. Schoonjans (1996)
Sp1‐mediated transcriptional activation is repressed by Sp3.
G. Hagen (1994)
Interaction of Oct-1 with TFIIB
H. Nakshatri (1995)
The protein Id: A negative regulator of helix-loop-helix DNA binding proteins
R. Benezra (1990)
Cloning and characterization of the promoter of the murine lipoprotein lipase-encoding gene: structural and functional analysis.
X. Hua (1991)
Developmental extinction of liver lipoprotein lipase mRNA expression might be regulated by an NF‐1‐like site
K. Schoonjans (1993)
DNA bending is induced by binding of the peroxisome proliferator-activated receptor gamma 2 heterodimer to its response element in the murine lipoprotein lipase promoter.
C. E. Robinson (1998)
Heparin decreases the degradation rate of lipoprotein lipase in adipocytes.
M. Cupp (1987)
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
J. Dignam (1983)
Drosophila Polycomb‐group regulated chromatin inhibits the accessibility of a trans‐activator to its target DNA.
D. Zink (1995)
A silencer element for the lipoprotein lipase gene promoter and cognate double- and single-stranded DNA-binding proteins.
Y. Tanuma (1995)
Developmentally regulated mRNAs in 3T3-adipocytes: analysis of transcriptional control
K. Cook (1985)
I kappa B interacts with the nuclear localization sequences of the subunits of NF-kappa B: a mechanism for cytoplasmic retention.
A. Beg (1992)
A simple phase-extraction assay for chloramphenicol acyltransferase activity.
B. Seed (1988)
Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases.
R. Eckel (1989)
The response of lipoprotein lipase to feeding and fasting. Evidence for posttranslational regulation.
M. Doolittle (1990)
A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.
P. Gunning (1987)
A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.
L. Chodosh (1986)
The structure and complete nucleotide sequence of the avian lipoprotein lipase gene.
D. A. Cooper (1992)
Organization of the human lipoprotein lipase gene and evolution of the lipase gene family.
T. Kirchgessner (1989)
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications
A. T. Andrews (1981)
DNA bending and orientation-dependent function of YY1 in the c-fos promoter.
S. Natesan (1993)

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