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Inhibition Of Apoptosis By Recombinant 30K Protein Originating From Silkworm Hemolymph.

E. H. Kim, H. J. Park, T. Park
Published 2003 · Biology, Medicine

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In a previous study, we reported that silkworm hemolymph inhibits apoptosis and that the anti-apoptotic component in silkworm hemolymph is a 30K protein. In this study, the 30K protein encoded by 30Kc6 was expressed in Escherichia coli. The recombinant 30K protein was expressed as an inclusion body, and the inclusion body was separated and refolded by affinity column chromatography using a 6xHis tag. We demonstrated that apoptosis is inhibited by supplementing the culture medium with this purified recombinant 30K protein. The recombinant 30K protein inhibited the virus- or chemical-induced apoptosis in human cells as well as insect cells. Apoptosis-inhibitory activity of recombinant 30K protein was comparable to that of whole silkworm hemolymph. The recombinant 30K protein can be effectively used to minimize cell death and consequently increase the productivity by extending the production time of host cells in commercial animal cell culture.
This paper references
10.1002/(SICI)1097-0290(19970720)55:2<439::AID-BIT21>3.0.CO;2-A
Extension of Sp2/0 hybridoma cell viability through interleukin-6 supplementation.
J. D. Chung (1997)
10.1084/JEM.184.6.2445
Different Interleukin-1β Converting Enzyme (ICE) Family Protease Requirements for the Apoptotic Death of T Lymphocytes Triggered by Diverse Stimuli
A. Sarin (1996)
10.1074/jbc.271.49.31075
Protease Involvement in Fodrin Cleavage and Phosphatidylserine Exposure in Apoptosis*
D. Vanags (1996)
10.1023/A:1018484309194
Efficient production of recombinant protein in Spodoptera frugiperda/AcNPV system utilizing silkworm hemolymph
S. Ha (2004)
10.1073/PNAS.88.17.7580
Transformed mammalian cells are deficient in kinase-mediated control of progression through the G1 phase of the cell cycle.
H. Crissman (1991)
10.1016/0167-4781(88)90086-3
Structures and expression of mRNAs coding for major plasma proteins of Bombyx mori.
N. Sakai (1988)
10.1083/JCB.135.5.1341
Genetic and metabolic status of NGF-deprived sympathetic neurons saved by an inhibitor of ICE family proteases
M. Deshmukh (1996)
10.1007/BF02931888
Quantitative measurement of general odorant using electroantennogram of male silkworm moth,Bombyx mori
Eung Sik Yun (2000)
10.1002/BIT.10191
Inhibition of sodium butyrate-induced apoptosis in recombinant Chinese hamster ovary cells by constitutively expressing antisense RNA of caspase-3.
N. S. Kim (2002)
10.1021/bp020001q
Inhibition of Human Cell Apoptosis by Silkworm Hemolymph
S. Choi (2002)
10.1006/BBRC.2001.5148
Isolation and characterization of an apoptosis-inhibiting component from the hemolymph of Bombyx mori.
E. Kim (2001)
10.1002/(SICI)1097-0290(19970405)54:1<1::AID-BIT1>3.0.CO;2-K
Prevention of hybridoma cell death by bcl-2 during suboptimal culture conditions.
N. Simpson (1997)
10.1016/S0006-291X(02)00746-5
Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells.
W. J. Rhee (2002)
10.1016/0014-4827(91)90166-R
Highly synchronous culture of fibroblasts from G2 block caused by staurosporine, a potent inhibitor of protein kinases.
K. Abe (1991)
10.1021/bp990093s
Kinetic Effect of Silkworm Hemolymph on the Delayed Host Cell Death in an Insect Cell‐Baculovirus System
Won Jong Rhee (1999)
10.1128/JVI.67.4.2168-2174.1993
An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif.
N. Crook (1993)
Flow cytometric analysis of the effect of silkworm hemolymph on the baculovirus-induced insect cell apoptosis
Rhee Won Jong (2001)
10.1006/EXCR.1994.1093
Induction of a common pathway of apoptosis by staurosporine.
R. Bertrand (1994)
10.1006/BBRC.2000.2592
Silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.
W. J. Rhee (2000)
10.1016/0005-2795(81)90013-1
Molecular properties and biosynthesis of major plasma proteins in Bombyx mori
Susumu Izumi (1981)
10.1016/S0167-7799(97)01159-1
Overcoming apoptosis: new methods for improving protein-expression systems.
A. Mastrangelo (1998)
10.1007/BF02940260
Anti-apoptosis engineering
Eun Jeong Kim (2003)
10.1007/BF02931887
High-efficient expression of porcine IL-2 with recombinant baculovirus infected silkworm,Bombyx mori
S. Inumaru (2000)
10.1006/EXCR.1999.4455
Escaping cell death: survival proteins in cancer.
M. Jäättelä (1999)



This paper is referenced by
10.1016/J.PROCBIO.2015.03.008
Enhancement of human erythropoietin production in Chinese hamster ovary cells through supplementation of 30Kc19-30Kc6 fusion protein
Hee Ho Park (2015)
dentification and characterization of a novel cell-penetrating peptide f 30 Kc 19 protein derived from Bombyx mori ee
H. Parka (2014)
Silkworm (Bombyx mori) and its constituents: A fascinating insect in science and research
M. Soumya (2017)
10.1007/s12257-015-0152-7
Inhibition of apoptosis in HeLa cell by silkworm storage protein 1, SP1
J. Lee (2015)
10.1002/BIT.20550
Beneficial effect of silkworm hemolymph on a CHO cell system: Inhibition of apoptosis and increase of EPO production.
S. Choi (2005)
10.1007/s12257-017-0424-5
Inhibition of Endoplasmic Reticulum Stress-induced Apoptosis by Silkworm Storage Protein 1
Yeon Ju Cha (2018)
10.1016/J.ENZMICTEC.2004.08.024
Increase of host cell longevity by the expression of 30K protein originating from silkworm hemolymph in an insect cell–baculovirus system
E. Kim (2004)
Two Novel 30 K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
W. Yu (2015)
Identification and Characterization of Cell-penetrating Peptide from 30Kc19 Protein and Its Application to Protein and Gene Delivery
박희호 (2014)
10.18388/ABP.2010_2436
Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction.
H. Chen (2010)
10.1016/J.IBMB.2012.07.006
The synthesis, transportation and degradation of BmLP3 and BmLP7, two highly homologous Bombyx mori 30K proteins.
Y. Zhang (2012)
10.1016/j.jinsphys.2016.06.008
Lysine acetylation stabilizes SP2 protein in the silkworm Bombyx mori.
Y. Zhou (2016)
10.1155/2013/145450
Expression and Functional Analysis of Storage Protein 2 in the Silkworm, Bombyx mori
W. Yu (2013)
Avaliação do perfil lipídico e proteico e caracterização dos hemócitos da hemolinfa de aranha marrom (Loxosceles intermedia)
A. V. Bednaski (2013)
10.1021/bp049841q
Establishment of N‐Acetylmannosamine (ManNAc) Analogue‐Resistant Cell Lines as Improved Hosts for Sialic Acid Engineering Applications
E. H. Kim (2004)
10.1007/s00726-009-0342-8
Shotgun proteomic analysis of the fat body during metamorphosis of domesticated silkworm (Bombyx mori)
H. Yang (2009)
10.1099/jgv.0.001087
Purification and characterization of immunogenic recombinant virus-like particles of porcine circovirus type 2 expressed in silkworm pupae.
Akitsu Masuda (2018)
10.1007/s12257-017-0283-0
Anti-apoptotic effects of the alpha-helix domain of silkworm storage protein 1
Ji Eun Baik (2017)
10.1002/(issn)1860-7314
Biotechnology Journal
Mathew M. Jessop (2019)
10.7852/IJIE.2013.26.1.031
Paralytic Peptide Binding Protein (PP-BP) Gene Expression During Egg Diapause and Its Multi-Gene Organization in the Silkworm Bombyx mori.
Sasibhushan Sirigineedi (2013)
10.1007/s12257-016-0588-4
Protective effects of silkworm hemolymph extract and its fractions on UV-induced photoaging
J. Park (2017)
10.1159/000320067
New Insight into the Mechanism of Lonomia obliqua Envenoming: Toxin Involvement and Molecular Approach
M. P. Alvarez Flores (2010)
10.1002/arch.21649
The stability and antiapoptotic activity of Bm30K-3 can be improved by lysine acetylation in the silkworm, Bombyx mori.
Ya-fei Ma (2019)
10.1002/arch.20313
The effect of calorie restriction on growth and development in silkworm, Bombyx mori.
Y. Li (2009)
10.1007/s13770-014-0405-0
Serum-free Media Supplement from Silkworm Gland for the Expansion of Mesenchymal Stem Cells
차현명 (2015)
10.1016/j.pestbp.2012.11.005
Transcriptional characteristics of gene expression in the midgut of domestic silkworms (Bombyx mori) exposed to phoxim.
Z. Gu (2013)
10.1016/j.jip.2019.02.008
Silkworm storage protein Bm30K-19G1 has a certain antifungal effects on Beauveria bassiana.
Ruilin Li (2019)
10.1016/J.PROCBIO.2017.01.017
Anti-apoptotic and Anti-oxidative Effects of Silkworm Storage Protein 1 in HeLa Cell
J. Lee (2015)
10.1021/bp049831p
Purification and Characterization of an Anti‐Apoptotic Protein Isolated from Lonomia obliqua Hemolymph
Alvaro P. B. Souza (2005)
10.1016/j.pestbp.2013.10.008
Characteristics of phoxim-exposed gene transcription in the silk gland of silkworms.
L. Ma (2013)
10.1016/J.CBPB.2006.10.101
Molecular properties and tissue distribution of 30K proteins as ommin-binding proteins from diapause eggs of the silkworm, Bombyx mori.
H. Sawada (2007)
10.1002/biot.201400253
Dimerization of 30Kc19 protein in the presence of amphiphilic moiety and importance of Cys-57 during cell penetration
Hee Ho Park (2014)
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