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Design And Evaluation Of New Ligands For Lysozyme Recovery By Affinity Thermoprecipitation

A. Vaidya, B. Lele, M. V. Deshmukh, M. Kulkarni
Published 2001 · Chemistry

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Abstract Ligands containing acetamido group and a spacer were conjugated with an acrylic monomer and copolymerized with N-isopropylacrylamide (NIPAM) to yield a thermoprecipitating polymer. The ability of the ligand to bind to lysozyme, which is the first step in the separation of lysozyme, is quantified in terms of I 50 , the ligand concentration required to achieve 50% of the maximum attainable inhibition of lysozyme. The copolymers containing acetamido groups inhibit lysozyme far more efficiently than the corresponding polymers containing N-acetylglucosamine, the natural inhibitor for lysozyme. The amount and activity of lysozyme recovered from the aqueous solution as well as lysozyme–ovalbumin mixture increased with the length and the hydrophilicity of the spacer. These polymers also exhibited better recyclability.
This paper references
10.1271/BBB1961.55.1683
N-Lower-fatty-acyl Derivatives of Chitosan as Adsorbents for Lysozyme and Chitinase
S. Hirano (1991)
10.1021/BC00019A006
Temperature-responsive bioconjugates. 1. Synthesis of temperature-responsive oligomers with reactive end groups and their coupling to biomolecules.
Y. Takei (1993)
10.1073/PNAS.57.4.1088
Studies on the enzymic activity of lysozyme, 3. The binding of saccharides.
J. Rupley (1967)
10.1016/0003-2697(85)90397-5
Chitin-coated Celite as an affinity adsorbent for high-performance liquid chromatography of lysozyme.
H. Yamada (1985)
Binding of hyaluronan to lysozyme at various pHs and salt concentrations.
M. van Damme (1991)
10.1002/BIT.260361103
Investigation of binding site density: Effects on the interaction between cibacron blue–dextran conjugates and lysozyme
A. G. Mayes (1990)
STUDIES ON THE ACTIVE CENTER OF TRYPSIN. THE BINDING OF AMIDINES AND GUANIDINES AS MODELS OF THE SUBSTRATE SIDE CHAIN.
M. Mares-Guia (1965)
10.1021/MA9816315
Controlled Synthesis of Amphiphilic Block Copolymers with Pendant N-Acetyl-d-glucosamine Residues by Living Cationic Polymerization and Their Interaction with WGA Lectin
Kenji Yamada (1999)
10.1016/0165-022X(93)90013-E
Batch isolation of hen egg white lysozyme with magnetic chitin.
I. Safarik (1993)
10.1016/S0032-3861(97)01024-0
Characterization of the LCST behaviour of aqueous poly(N-isopropylacrylamide) solutions by thermal and cloud point techniques
Costas J. Boutris (1997)
10.1073/PNAS.56.1.26
On the binding of chitin oligosaccharides to lysozyme.
F. Dahlquist (1966)
10.1002/(SICI)1521-3773(19981102)37:20<2754::AID-ANIE2754>3.0.CO;2-3
Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors.
M. Mammen (1998)
10.1098/rspb.1967.0035
Crystallographic studies of the activity of hen egg-white lysozyme
C. Blake (1967)
10.1002/BIT.260411113
Affinity thermoprecipitatin: Contribution of the efficiency of ligand–protein interaction and access of the ligand
I. Galaev (1993)
10.1002/BIT.260340406
Biospecific adsorption of lysozyme onto monoclonal antibody ligand immobilized on nonporous silica particles
A. Liapis (1989)
10.1093/OXFORDJOURNALS.JBCHEM.A127803
THE ENZYME-SUBSTRATE COMPLEX IN A MURAMIDASE CATALYZED REACTION. I. DIFFERENCE SPECTRUM OF COMPLEX.
Keiko Hayashi (1963)
10.1016/0005-2795(67)90007-4
Inhibition of lysozyme by derivatives of D-glucosamine. I.
A. Neuberger (1967)
10.1007/BF00255084
Thermal stability of high-molecular-weight polyacrylamide aqueous solutions
G. Muller (1981)
Design synthesis and evaluation of polymers for affinity based enzyme separations
A. Vaidya (2000)
10.1016/0005-2795(74)90121-4
Separation of proteins with polyacrylic acids.
M. Sternberg (1974)
10.1098/rspb.1967.0038
The binding and cleavage by lysozyme of N-acetylglucosamine oligosaccharides
J. Rupley (1967)
10.1016/S0376-7388(96)00207-4
Fractionation of natural and model egg-white protein solutions with modified and unmodified polysulfone UF membranes
N. Ehsani (1997)
10.1016/0927-7765(96)01281-7
Biotin-modified submicron latex particles for affinity precipitation of avidin
C. Chern (1996)
10.1006/ABBI.1994.1134
Binding properties of glycosaminoglycans to lysozyme--effect of salt and molecular weight.
M. van Damme (1994)
10.1016/0141-0229(93)90122-I
Thermoreactive water-soluble polymers, nonionic surfactants, and hydrogels as reagents in biotechnology.
Galaev IYu (1993)
10.1002/(SICI)1097-0290(19971220)56:6<610::AID-BIT3>3.0.CO;2-Q
Macroporous chitin affinity membranes for lysozyme separation.
E. Ruckenstein (1997)
10.1002/(SICI)1097-0290(19990820)64:4<418::AID-BIT4>3.0.CO;2-Y
Enhancing ligand-protein binding in affinity thermoprecipitation: elucidation of spacer effects
Vaidya (1999)
10.1038/NBT0990-854
Cell Culture on a Thermo-Responsive Polymer Surface
T. Takezawa (1990)
10.1016/0005-2744(69)90397-0
The dependence of lysozyme activity on pH and ionic strength.
R. Davies (1969)
10.1177/1045389X9300400207
Interactions between Thermosensitive Hydrogel Microspheres and Proteins
Keiji Fujimoto (1993)
Ph
李幼升 (1989)
10.1016/S0021-9673(98)00656-6
Affinity precipitation – an alternative to fluidized bed adsorption?
M. Eggert (1998)
10.1063/1.476663
Molecular tailoring of thermoreversible copolymer gels: Some new mechanistic insights
M. V. Badiger (1998)
10.1016/S1389-1723(99)89013-6
Kinetic study on thermal denaturation of hen egg-white lysozyme involving precipitation.
D. Nohara (1999)
10.1016/S0376-7388(00)82334-0
Separation of proteins from polyelectrolytes by ultrafiltration
Andrea G. Bozzano (1991)
10.1016/S0015-1882(97)86674-8
Separation of binary protein mixtures by ultrafiltration
E. Iritani (1997)
10.1295/POLYMJ.19.297
Intrinsic Viscosity-Molecular Weight Relationships for Poly(N-isopropylacrylamide) Solutions
S. Fujishige (1987)
10.1016/0014-5793(75)80720-4
Purification of lysozyme by affinity chromatography
E. Junowicz (1975)
10.1016/0927-7765(95)01235-4
Characterization of pH-sensitive polymeric supports for selective precipitation of proteins
C. Chern (1996)
10.1016/s0021-9258(18)43040-2
Direct crystallization of lysozyme from egg white and some crystalline salts of lysozyme.
G. Alderton (1946)



This paper is referenced by
10.1205/FBP.05133
Integration of Affinity Precipitation with Partitioning Methods for Bioseparation of Chitin Binding Lectins
S. Teotia (2006)
10.1016/J.PROCBIO.2012.01.019
Protein precipitation using an anionic surfactant
Shu Ian Cheng (2012)
10.1002/9781118181249.CH2
Smart Surfaces for Point‐of‐Care Diagnostics
M. A. Nash (2012)
10.1007/s12010-015-1742-8
Microbial Transglutaminase Separation by pH-Responsive Affinity Precipitation with Crocein Orange G as the Ligand
Zhaoyang Ding (2015)
NMR Investigations on Structure, Dynamics and Function of VAT-N and DOTATOC
M. V. Deshmukh (2004)
10.3762/bjoc.6.66
RAFT polymers for protein recognition
A. Tominey (2010)
10.1134/S0965545X12060090
Interpolyelectrolyte complexes of soybean peroxidase with thermoresponsive copolymers
T. Burova (2012)
10.1002/btpr.671
Protein recovery from surfactant precipitation
Shu Ian Cheng (2011)
10.1021/bp025789r
Reverse Micellar Extraction and Precipitation of Lysozyme Using Sodium Di(2‐ethylhexyl) Sulfosuccinate
Youn-Ok Shin (2003)
Adsorptive recovery of nanoparticulate protein products : physical and biochemical characterisation of candidate solid phases
S. L. Williams (2002)
10.1111/J.1365-2621.2003.TB05716.X
Selective Precipitation of Lysozyme from Egg White Using AOT
Y. Shin (2003)
10.1080/07388551.2017.1312266
Downstream protein separation by surfactant precipitation: a review
F. Wong (2018)
10.1016/j.jchromb.2010.03.001
Synthesis of thermo-sensitive copolymer with affinity butyl ligand and its application in lipase purification.
L. Zhou (2010)
10.1002/APP.27211
Preparation of novel amphiphilic copolymer microspheres and their drug-release and glucose-sensitive properties
Y. Tang (2008)
10.1142/S0217984913410285
PURIFICATION OF LYSOZYME BY INTRINSICALLY SHIELDED HYDROGEL BEADS
C. Li (2013)
10.1039/c5an00201j
Magnetic multiwall carbon nanotubes modified with dual hydroxy functional ionic liquid for the solid-phase extraction of protein.
J. Chen (2015)
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