← Back to Search
High Protonic Potential Actuates A Mechanism Of Production Of Reactive Oxygen Species In Mitochondria
S. S. Korshunov, V. Skulachev, A. Starkov
Published 1997 · Chemistry, Medicine
Save to my Library
Download via 🐼 PaperPanda Download via oaDOI Download via OAB Download via LibKey Download via Google Google ScholarAnalyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Formation of H2O2 has been studied in rat heart mitochondria, pretreated with H2O2 and aminotriazole to lower their antioxidant capacity. It is shown that the rate of H2O2 formation by mitochondria oxidizing 6 mM succinate is inhibited by a protonophorous uncoupler, ADP and phosphate, malonate, rotenone and myxothiazol, and is stimulated by antimycin A. The effect of ADP is abolished by carboxyatractylate and oligomycin. Addition of uncoupler after rotenone induces further inhibition of H2O2 production. Inhibition of H2O2 formation by uncoupler, malonate and ADP+Pi is shown to be proportional to the ΔΨ decrease by these compounds. A threshold ΔΨ value is found, above which a very strong increase in H2O2 production takes place. This threshold slightly exceeds the state 3 ΔΨ level. The data obtained are in line with the concept [Skulachev, V.P., Q. Rev. Biophys. 29 (1996), 169–202] that a high proton motive force in state 4 is potentially dangerous for the cell due to an increase in the probability of superoxide formation.
This paper references
Respiratory chain linked H2O2 production in pigeon heart mitochondria
G. Loschen (1971)
The mitochondrial generation of hydrogen peroxide. General properties and effect of hyperbaric oxygen.
A. Boveris (1973)
Mitochondrial H2O2 formation: Relationship with energy conservation
G. Loschen (1973)
Safranine as a probe of the mitochondrial membrane potential
K. Åkerman (1976)
Evaluation of the horseradish peroxidase-scopoletin method for the measurement of hydrogen peroxide formation in biological systems.
A. Boveris (1977)
Biochemistry (Moscow) 59, 813-818
A. Boveris (1977)
Biochemistry (Moscow) 59, 813^818
A. Boveris (1977)
Do mitochondria produce oxygen radicals in vivo?
H. Nohl (1978)
Safranine as membrane potential probe in rat liver mitochondria.
A. Zanotti (1980)
The relationship between extramitochondrial Ca2+ concentration, respiratory rate, and membrane potential in mitochondria from brown adipose tissue of the rat.
J. Nedergaard (1983)
[Generation of superoxide radicals by the mitochondrial respiratory chain of isolated cardiomyocytes].
K. Kashkarov (1994)
6‐Ketocholestanol abolishes the effect of the most potent uncouplers of oxidative phosphorylation in mitochondria
A. Starkov (1994)
Role of uncoupled and non-coupled oxidations in maintenance of safely low levels of oxygen and its one-electron reductants.
V. Skulachev (1996)
Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c
X. Liu (1996)
Loss of Function of Cytochrome c in Jurkat Cells Undergoing Fas-mediated Apoptosis*
Anja Krippner (1996)
Dependence of H2O2 Formation by Rat Heart Mitochondria on Substrate Availability and Donor Age
R. Hansford (1997)
Prevention of Apoptosis by Bcl-2: Release of Cytochrome c from Mitochondria Blocked
Jie Yang (1997)
The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of Apoptosis
R. Kluck (1997)
6-Ketocholestanol is a recoupler for mitochondria, chromatophores and cytochrome oxidase proteoliposomes.
A. Starkov (1997)
Role for Bcl-xL as an inhibitor of cytosolic cytochrome C accumulation in DNA damage-induced apoptosis.
S. Kharbanda (1997)
This paper is referenced by
Interplay between mitochondria and reactive oxygen and nitrogen species in metabolic adaptation to hypoxia in facioscapulohumeral muscular dystrophy: potential therapeutic targets
P. Heher (2021)
Parenteral Nutrition and Cardiotoxicity.
Johan van Nispen (2021)
ATPIF1 maintains normal mitochondrial structure which is impaired by CCM3 deficiency in endothelial cells
Kang Wang (2021)
The Relationship between Mitochondrial Reactive Oxygen Species Production and Mitochondrial Energetics in Rat Tissues with Different Contents of Reduced Coenzyme Q
K. Dominiak (2021)
Molecular and Supramolecular Structure of the Mitochondrial Oxidative Phosphorylation System: Implications for Pathology
S. Nesci (2021)
Multiple Mechanisms Regulate Eukaryotic Cytochrome C Oxidase
R. Ramzan (2021)
Oxidative phosphorylation is required for powering motility and development of the sleeping sickness parasite Trypanosoma brucei within the tsetse fly vector
Caroline E. Dewar (2021)
Mitochondrial K+ Transport: Modulation and Functional Consequences
O. Pereira (2021)
Vanillic acid retains redox status in HepG2 cells during hyperinsulinemic shock using the mitochondrial pathway
Sreelekshmi Mohan (2021)
Integrating Mitochondrial Aerobic Metabolism into Ecology and Evolution.
Rebecca E Koch (2021)
Modulating the Heat Stress Response to Improve Hyperthermia-Based Anticancer Treatments
Enzo M. Scutigliani (2021)
Is mitochondrial reactive oxygen species production proportional to oxygen consumption? A theoretical consideration
C. Hou (2021)
Neuroprotective Potential of Mild Uncoupling in Mitochondria. Pros and Cons
D. Zorov (2021)
Unique metabolic phenotype and its transition during maturation of juvenile male germ cells
A. Voigt (2021)
The BH3 mimetic (±) gossypol induces ROS-independent apoptosis and mitochondrial dysfunction in human A375 melanoma cells in vitro
Lisa Haasler (2021)
Protective Effects of Shenfuyixin Granule on H2O2-Induced Apoptosis in Neonatal Rat Cardiomyocytes
Xinlu Wang (2021)
Elesclomol-induced increase of mitochondrial reactive oxygen species impairs glioblastoma stem-like cell survival and tumor growth
M. Buccarelli (2021)
Effects of Matrix pH on Spontaneous Transient Depolarization and Reactive Oxygen Species Production in Mitochondria
J. Aklima (2021)
Uridine treatment prevents myocardial injury in rat models of acute ischemia and ischemia/reperfusion by activating the mitochondrial ATP-dependent potassium channel
I. B. Krylova (2021)
Perfluorodecanoic acid induces meiotic defects and deterioration of mice oocytes in vitro.
Shu-Zi Deng (2021)
Cytostatic Effect of a Novel Mitochondria-Targeted Pyrroline Nitroxide in Human Breast Cancer Lines
Kitti Andreidesz (2021)
Endothelial response to glucose: dysfunction, metabolism, and transport.
A. Clyne (2021)
Saccharomyces boulardii modulates oxidative stress and renin angiotensin system attenuating diabetes-induced liver injury in mice
Leticia Barssotti (2021)
GDF11 alleviates secondary brain injury after intracerebral hemorrhage via attenuating mitochondrial dynamic abnormality and dysfunction
Anqi Xiao (2021)
The Potential Role of Creatine in Vascular Health
H. Clarke (2021)
The Uprising of Mitochondrial DNA Biomarker in Cancer
Siti Zulaikha Nashwa Mohd Khair (2021)
Cytoskeletal Arrest: An Anoxia Tolerance Mechanism
Alexander Myrka (2021)
The Small GTPase Cdc42 Negatively Regulates the Formation of Neutrophil Extracellular Traps by Engaging Mitochondria
H. Tackenberg (2021)
Antioxidant Synergy of Mitochondrial Phospholipase PNPLA8/iPLA2γ with Fatty Acid–Conducting SLC25 Gene Family Transporters
M. Jabůrek (2021)
The stress response protein REDD1 as a causal factor for oxidative stress in diabetic retinopathy.
W. P. Miller (2021)
An update on methods and approaches for interrogating mitochondrial reactive oxygen species production
R. Mailloux (2021)
Ammonium Glycyrrhizinate Prevents Apoptosis and Mitochondrial Dysfunction Induced by High Glucose in SH-SY5Y Cell Line and Counteracts Neuropathic Pain in Streptozotocin-Induced Diabetic Mice
L. Ciarlo (2021)See more