← Back to Search
Cytochrome C Release And Caspase Activation During Menadione‐induced Apoptosis In Plants
Y. Sun, Y. Zhao, X. Hong, Zhong He Zhai
Published 1999 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
We report here the detection of the release of cytochrome c from mitochondria into the cytosol during menadione‐induced apoptosis in tobacco protoplasts. Western blot analysis indicated that the caspase specific inhibitors AC‐DEVD‐CHO (Ac‐Asp‐Glu‐Val‐Asp‐aldehyde) and AC‐YVAD‐CHO (N‐acetyl‐Try‐Val‐Ala‐aspartinal) inhibited the degradation of a caspase 3 specific substrate PARP (poly(ADP‐ribose) polymerase), and they had no effect on the release of cytochrome c. Further study showed that menadione could not induce apoptosis of mouse liver nuclei in tobacco cytosol extract containing no mitochondria. However, when cytochrome c or mitochondria was added into the cytosol extract, apoptosis of mouse liver nuclei and the degradation of PARP could both be detected. The results provide strong evidence that menadione can induce apoptosis in tobacco protoplasts via the release of cytochrome c from mitochondria into the cytosol.
This paper references
Characterization of anArabidopsis thaliana cDNA homologue to animal poly(ADP‐ribose) polymerase
L. Lepiniec (1995)
Menadione-induced apoptosis and the degradation of lamin-like proteins in tobacco protoplasts
Y.-L. Sun (1999)
Cytochrome c in the apoptotic and antioxidant cascades
V. Skulachev (1998)
Purification and Characterization of an Interleukin-1β-converting Enzyme Family Protease That Activates Cysteine Protease P32 (CPP32)*
X. Liu (1996)
Apoptosis: A Functional Paradigm for Programmed Plant Cell Death Induced by a Host-Selective Phytotoxin and Invoked during Development.
H. Wang (1996)
Programmed Cell Death in Plants.
R. Pennell (1997)
Induction of Apoptotic Program in Cell-Free Extracts: Requirement for dATP and Cytochrome c
X. Liu (1996)
Apoptosis by Death Factor
S. Nagata (1997)
Poly(ADP-ribose) polymerase in plant nuclei.
Y. Chen (1994)
Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade
Peng Li (1997)
Prevention of Apoptosis by Bcl-2: Release of Cytochrome c from Mitochondria Blocked
J. Yang (1997)
The NB-ARC domain: a novel signalling motif shared by plant resistance gene products and regulators of cell death in animals
E. A. Biezen (1998)
The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of Apoptosis
R. Kluck (1997)
Specific cleavage of the retinoblastoma protein by an ICE‐like protease in apoptosis.
R. Jänicke (1996)
Role of CED-4 in the activation of CED-3
A. Chinnaiyan (1997)
Advances in apoptosis research.
M. Peter (1997)
Bcl-2 functions in an antioxidant pathway to prevent apoptosis
D. Hockenbery (1993)
Activation of Caspase-2 in Apoptosis*
H. Li (1997)
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
A. Wyllie (1980)
Apoptosis of mouse liver nuclei induced in the cytosol of carrot cells
Y Zhao (1999)
Nuclei from Rat Liver: Isolation Method That Combines Purity with High Yield
G. Blobel (1966)
This paper is referenced by
HYPERSENSITIVE CELL DEATH IN PLANTS – ITS MECHNISMS AND ROLE IN PLANT DEFENCE AGAINST PATHOGENS
D. Tsankov (2006)
Molecular Mechanisms Underlying Vascular Development
Jae-Hoon Jung (2008)
The characterisation of Late Embryogenesis Abundant 5 and Ethylene Responsive Factor 109 functions in plants growth and stress tolerance
D. Shaw (2016)
La muerte celular programada en las plantas: ¿Es semejante a la "apoptosis" en animales?
Yornayser Pérez Delgado (2007)
Hybrid Necrosis in \Vheat-A Genetic System Showing Reduced Capacity to Detoxify Reactive Oxygen Species Leading to Programmed Cell Death
G. Sharma (2003)
Heat Shock Induces Programmed Cell Death in Wheat Leaves
T. Fan (2004)
Anoxia pretreatment protects soybean cells against H2O2‐induced cell death: possible involvement of peroxidases and of alternative oxidase
Y. Amor (2000)
Monitoring programmed cell death triggered by mild heat shock in soybean-cultured cells.
Anna Zuppini (2006)
Analysis of the function of cathepsin B as a regulator of plant programmed cell death
Yao Min Cai (2013)
OsVPE3 Mediates GA-induced Programmed Cell Death in Rice Aleurone Layers via Interacting with Actin Microfilaments
Heting Zhang (2020)
Multiple mediators of plant programmed cell death: Interplay of conserved cell death mechanisms and plant‐specific regulators
F. Hoeberichts (2003)
Plant Programmed Cell Death, Ethylene and Flower Senescence
E. Woltering (2005)
KCl induces a caspase-independent programmed cell death in the unicellular green chlorophyte Chlamydomonas reinhardtii (Chlorophyceae)
Sirisha L. Vavilala (2016)
Cell physiology of mortality and immortality in a Nicotiana interspecific F1 hybrid complies with the quantitative balance between reactive oxygen and nitric oxide.
Takumi Yamamoto (2017)
Programmed cell death and tissue remodeling in plants
A. Gunawardena (2007)
Perturbation of NgTRF1 Expression Induces Apoptosis-Like Cell Death in Tobacco BY-2 Cells and Implicates NgTRF1 in the Control of Telomere Length and Stability
S. Yang (2004)
Programmed cell death of the nucellus during Sechium edule Sw. seed development is associated with activation of caspase-like proteases.
L. Lombardi (2007)
Menadione sodium bisulphite mediated growth, secondary metabolism, nutrient uptake and oxidative defense in okra (Abelmoschus esculentus Moench) under cadmium stress.
Rizwan Rasheed (2018)
Programmed cell death or apoptosis: Do animals and plants share anything in common
N. Jan (2013)
Plant programmed cell death: a common way to die.
A. Danon (2000)
Authors for correspondence
A. Stace (2017)
NaSIPP mediates self-incompatibility in Nicotiana 1 2 3 4 5
Liliana E. Garcia-Valencia (2017)
The presence and subcellular localization of caspase 3‐like proteinases in plant cells
H. Korthout (2000)
The cellular and molecular biology of conifer embryogenesis.
J. Cairney (2007)
Molecular analysis of menadione-induced resistance against biotic stress in Arabidopsis.
Andrés Borges (2009)
The LCB2 subunit of the sphingolip biosynthesis enzyme serine palmitoyltransferase can function as an attenuator of the hypersensitive response and Bax-induced cell death.
Yun-zhe Gan (2009)
A possible involvement of plasma membrane NAD(P)H oxidase in the switch mechanism of the cell death mode from apoptosis to necrosis in menadione-induced cell injury.
E. Niemczyk (2004)
The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c Release
J. Balk (2001)
Mitochondria-Nucleus Interactions: Evidence for Mitochondrial Retrograde Communication in Plant Cells
D. Rhoads (2004)
Involvement of Chloroplasts in the Programmed Death of Plant Cells
V. Samuilov (2004)
Protective role of nitric oxide during hydrogen peroxide-induced oxidative stress in tobacco plants
L. V. Dubovskaya (2007)
Mitochondrial Regulation of Plant Programmed Cell Death
Mark Diamond (2011)See more